DHPS (NM_013406) Human Untagged Clone

CAT#: SC115255

DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 2



  "NM_013406" in other vectors (4)

CNY 2,400.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-DHPS mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DHS; DS; MIG13; NEDSSWI
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_013406, the custom clone sequence may differ by one or more nucleotides


ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAGCTCGACGT
TGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGA
GGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATC
GAGAAGAAGCTGGAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCAC
TTACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCG
CTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC
AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAGAACGGGATCA
ATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAGGACTGGCTGATGCCCATTCT
GGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTG
GGCAAGGAGATCAACAACCCAGAGTCCGTGTATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTC
CCGCACTTACAGACGGCTCGCTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCT
GGACATCGTTGAGGCCCAGGAGTTTGATGGCTCTGACTCAGGTGCCCGACCAGACGAGGCTGTCTCCTGG
GGCAAGATCCGGGTGGATGCACAGCCCGTCAAGGTCTATGCTGACGCCTCCCTGGTCTTCCCCCTGCTTG
TGGCTGAAACCTTTGCCCAGAAGATGGATGCCTTCATGCATGAGAAGAACGAGGACTGA


>OriGene 5' read for NM_013406 unedited
GTGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCGGGGTT
TAACGCGTTTCTGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCGAGAGCCGGC
TCTAGGCGCATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTG
CTAAAGCACAGCTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAAC
CGCGGTGTGAATTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACC
AACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTG
TCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGC
ACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGC
TACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAA
GACCTCATCAAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAG
CTCCGGGAGAACGGGATCAATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGC
AAGTTTGAGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGACACAGAGG
GTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTGGCCAGGAGATCACACCCAGAGT
CCGTGTATTACTGGCCCAGAGACCAATTCCTGTGGTTTAGTCCGCCTTACGACGGCTCGC
TGGCGACATGATCTTCTTCATTCTCAGGACCGGGGCCTGGTCTGGAATTATN
>OriGene 3' read for NM_013406 unedited
GGTACTCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTGACTTCAGATACCATCTTATTCTAGAGACGTAGCTGACCAAAAATAGGGGAGG
GGCTGGGTCTGCAAATTAATAAATAAAAGAGGGGGTAAGACCTTCCTGGGACCGCAGCCG
CTCAGTCCTCGTTCTTCTCATGCATGAAGGCATCCATCTTCTGGGCAAAGGTTTCAGCCA
CAAGCAGGGGGAAGACCAGGGAGGCGTCAGCATAGACCTTGACGGGCTGTGCATCCACCC
GGATCTTGCCCCAGGAGACAGCCTCGTCTGGTCGGGCACCTGAGTCAGAGCCATCAAACT
CCTGGGCTGTGTTGATGTAAACAGCGTAGTCGGCCCCGTTCCGCATGAGGTTGGCATTGG
CAATGTGGTGCTTGACCACGCCCCCGCCCAGAATGATCATCCCAGTGCACTTGGCAAAGA
TGGCCTGTGTGTTGATGAGCCTCAGGTCCTCAACGATGTCCAGGACCAGGCCCGGGTTCT
TGTAGGAATGGAAGAAGATCATGTCGCCCAGCGAGCCGTCTGTAAGTGCGGGACTAAACA
CAGGGATGTGGTTCTTCTGGGCCCAGTAATACACGGACTCTGGGTTGTTGATCTCCTTGC
CCAGCCGGGCGATCATCTTAGAAGGCGTCCACTTTACACCCTCTGTGTTCTGCTCCATCA
CCATCTGGTCCAGAATGGGCATCAGCCAGTCCTCAAACTTGCAGTAATTCTCATTGGGCA
CCAGCAGGTTTCCGATCCTATTGATCCCGTTCTCCCGGACTCCTTCCCCCTGAGCTAAAC
TCGCCAAGTTGTGGGCGCCAGGCCTTGATGAGGTCTTCCTNCACGCGCCAGCTGTGGTCA
CAATACGTCACCATGTTG
Restriction Sites NotI-NotI     
ACCN NM_013406
Insert Size 1430 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013406.1, NP_037538.1
RefSeq Size 1210 bp
RefSeq ORF 969 bp
Locus ID 1725
UniProt ID P49366
Domains DS
Gene Summary This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]
Transcript Variant: This variant (2) lacks an exon and uses an alternate splice site in the 3' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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