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PD-L1 (CD274) (NM_014143) Human Untagged Clone
CAT＃: SC115168
PD-L1 / CD274 (untagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274)
CNY 3,600.00
| Cited in 5 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115168 sequence for NM_014143 edited (data generated by NextGen Sequencing)
ATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACT GTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGC AAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAG GATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGC TACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAG ATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGT GCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGA ATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTAC CCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACC ACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAAC ACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCAT ACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCAC TTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGT TTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAG AAGCAAAGTGATACACATTTGGAGGAGACGTAA Clone variation with respect to NM_014143.3 >OriGene 5' read for NM_014143 unedited
CGGGCCCCNNNNCCCCAACCNCCCCCGGTTCAAATTTGTATACGACTCACTATAGGCGGC CGCGAATTCGCACGAGGCACCAGCCGCGCTTCTGTCCGCCTGCAGGGCATTCCAGAAAGA TGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTG TCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCA AATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGG ATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCT ACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGNGAAATGCTGCACTTCAGA TCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTG CCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAA TTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCANGCTGANGGCTACC CCAAGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCAC CACCAATTCCAAGAGAGAGGAGAAGCTTTTCATGTGACCACACACTGAGAAATCACACAN NCACTATGAGATTTTCTACTGCACTTTTANGAGAATAGATCCCTGAGAAAACATACAGCT GAATTGGTCATCCCAGAACTACCTCTGGCACATTCTTCAATGAAAGGACTCACTTGGTAA TNCTGGGAGCCATCCTATTATGCCCTTGGTGTAACACTGACATTCATTTTTNCGTTTAAG AAAGGNAGAATGATGNATGTGAAAAAATGTGCATNCCAGATACAANACTCAAGAAGCAAG TGATACACATTGGNAGGANACAGTATCCAGCATTGGAACTTCTGATCTTCAAGCGGGATN CTCAACCTGGG >OriGene 3' genomic read for NM_014143 unedited
TCGACCATGGAATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATG CCACCCGGGTTCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAAGTAACAAAATAAATAAAAATATT TTATTAAATTAATGCAGGTACAAAAAGTGACAATCAAATGCAAAATTAGGTATCATCTCT GCCTATGCCATTTACGATGAAACATGAGACAAAAGGGATAAAGTGCCTTACAAATCCAAC ACCACAGGGAGGAGTTAGGACTTAGGAATAGACTGAGTAGACTATGTGCCTTGCTCAGCC ACAATTCTTGCCTGTAATTCACACAAAGAACACTGTCACACCAATTACTGTACAAATGCA CATGTACAAACAGATAACACAAGGAGCTCTGTTGGAGACACTGTTTCTTCAGCCTTGACA TGTGGCAAAGCCAAGGTACTCCTTGTCATTGACACCAGAATATGGCCAAGAGGGAAAGGA AACTCTGGGATCTCCCAGGGCATCTGAATCTCGAAACCTCCAGGAAGCCTCTTAAGAGGG CTGTGTAGTGATGACAGCTGGTGGCATTCAAGGGTTCAAGCACAACGAATGAGGCTTTTC TGGCACAGGGTAGCTAGCAGTCAAGGTACACTGCCGGAATTTCCAGAAAGAAAATGTGAT TTTGCAAGTACAGCATCAAAGTGAAGTTTATACTTGACAGAAAGCAGAAAACAAAAGTTC CAGACATGATTCTGTCATACAGAGTTAATAATAAGATTGCTTTTTAGGACTAGATTGACT CAGTGCACCCTGGAGAGCCCATGGGTCTCCTTTTGAGCAACATAACAAATCCAACTCTGT CCTAGACAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014143 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014143.2, NP_054862.1 |
| RefSeq Size | 1553 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 29126 |
| UniProt ID | Q9NZQ7 |
| Domains | ig, IG |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| Gene Summary | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Additive Effect of CD73 Inhibitor in Colorectal Cancer Treatment With CDK4/6 Inhibitor Through Regulation of PD-L1
,null,
Cellular and Molecular Gastroenterology and Hepatology
,PubMed ID 35843546
[PD-L1]
|
|
PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade
,Trefny, M;Kaiser, M;Stanczak, M;Herzig, P;Savic, S;Wiese, M;Lardinois, D;Läubli, H;Uhlenbrock, F;Zippelius, A;,
Cancer Immunol Immunother
,PubMed ID 32296919
[PD-L1]
|
|
BRAFV600E-induced, tumor intrinsic PD-L1 can regulate chemotherapy-induced apoptosis in human colon cancer cells and in tumor xenografts
,Feng, D;Qin, B;Pal, K;Sun, L;Dutta, S;Dong, H;Liu, X;Mukhopadhyay, D;Huang, S;Sinicrope, FA;,
Oncogene
,PubMed ID 31406255
[PD-L1]
|
|
Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
,Cherkassky, L;Morello, A;Villena-Vargas, J;Feng, Y;Dimitrov, DS;Jones, DR;Sadelain, M;Adusumilli, PS;,
J. Clin. Invest.
,PubMed ID 27454297
[PD-L1]
|
|
MiR-570 inhibited the cell proliferation and invasion through directly targeting B7-H1 in hepatocellular carcinoma
,Guo, W;Tan, W;Liu, S;Huang, X;Lin, J;Liang, R;Su, L;Su, Q;Wang, C;,
Tumour Biol.
,PubMed ID 26084609
[PD-L1]
|
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Resources
Other Versions
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| RC213071 | PD-L1 / CD274 (Myc-DDK-tagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274) |
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| RC213071L2 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged |
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| RC213071L3 | Lenti ORF clone of Human CD274 molecule (CD274), Myc-DDK-tagged |
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| RC213071L4 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged |
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| RG213071 | PD-L1 / CD274 (tGFP-tagged) - Human PD-L1 / CD274 molecule (PD-L1 / CD274) |
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