MAN1 (LEMD3) (NM_014319) Human Untagged Clone

CAT#: SC115036

LEMD3 (untagged)-Human LEM domain containing 3 (LEMD3), transcript variant 1



  "NM_014319" in other vectors (6)

CNY 6,680.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
Anti-LEMD3 (MAN1) mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MAN1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_014319, the custom clone sequence may differ by one or more nucleotides


ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCCGTT
ACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAGAAGCTGAAGAAGCTTCG
AGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAACAAGACGCGGAACAGTAATAACAATAAC
ACGGCAGCCGCCACGGTCGCAGCCGCGGGACCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGG
TCTCGGGCGACCTCTCCTACTTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAG
CCTCCTGGGAGGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGC
TCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGACCGGGCTT
CGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAGGTGACTAACAGCAACTC
TGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGGCCGGCGGGCCCCGAGCTGCAGACCCCG
CCGGGGAAAGATGGAGCAGTGGAGGACGAGGAAGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGAGA
CCGAGGAGCCGCTCTGGGCGAGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGGAAAA
CTATTCGGACTCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTAAAGGACGACTCC
CTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCTAAATCGGCCGGCG
GCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGACAGGGCGGCGGCTGCCGGGAG
TCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCGGAGCAGGGAGGAGGGTGTGATCAAGTGGAC
TCCAGCCCCGTTCCTAGATACCGTGTTAACGCTAAGAAACTGACCCCTCTCCTGCCCCCGCCACTTACTG
ACATGGACTCAACCTTGGATTCGTCAACAGGCTCCCTTCTGAAAACCAATAATCATATTGGCGGTGGGGC
CTTCAGTGTGGACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCGGCGGTGGCCGCCTCTAGT
TCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTGAAAAACACATACAACAAAC
CGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAGGAGGTGTCCCCAACAGGGAG
TTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCTGCCTGCTTATTTTTCCTAATACTGGGACTG
ACTTACCTAGGAATGAGAGGGACAGGAGTATCTGAGGATGGAGAACTCAGCATAGAAAACCCCTTTGGTG
AAACATTTGGAAAAATACAAGAAAGTGAAAAAACTCTTATGATGAACACATTATATAAGCTTCATGATCG
ATTGGCACAGCTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGAACGCTTTCTGTTCAAGAGGCA
GCTGCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAACACTTCATTGCAGTGGATCTTAG
AAAATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAGGAAGAATTGACAAATATAACTGA
TGTGCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTTTGGTGTCGTTTTCGACGTGCTTTTGTTACT
GTAACTCACAGATTATTGTTGTTATGCTTAGGTGTAGTGATGGTTTGTGTCGTTCTGCGTTACATGAAAT
ATCGATGGACAAAAGAAGAGGAGGAAACAAGGCAGATGTATGATATGGTGGTAAAGATTATAGATGTTTT
ACGAAGTCATAATGAAGCCTGCCAGGAAAACAAAGATTTACAACCTTACATGCCTATTCCACATGTACGC
GATTCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAAGTCTGGGATAGAGCTGTTGACTTCCTTG
CTGCTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATAGGTGGTGCAGATTTTCTGGTTTGGCGGTG
GATCCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATACCTTCTAAAGTATGGCAAGGTCAAGCATTT
CATTTAGATAGAAGAAATTCACCACCAAATAGTTTGACACCGTGTCTAAAGATTCGGAATATGTTTGATC
CCGTTATGGAAATAGGGGATCAGTGGCATTTGGCAATTCAAGAAGCAATTTTAGAAAAATGCAGTGATAA
TGATGGCATTGTTCACATTGCAGTAGACAAAAATTCACGTGAGGGTTGTGTATATGTTAAATGTCTGTCT
CCAGAATATGCTGGAAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTGATGGGAAATTGGTTACAGTAA
AATATTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCTCTCACTTCCAACACTCCATTGAAGCC
ATCAAATAAACATATGAACTCCATGTCTCATCTTCGTCTTCGGACTGGCCTAACCAATTCTCAAGGAAGT
TCCTGA


>OriGene 5' read for NM_014319 unedited
NGGGTCGGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGAGAAAAG
GCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTC
CGCCGTTACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAG
AAGCTGAAGAAGCTTCGAGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAAC
AAGACGCGGAACAGTAATAACAATAACACGGCAGCCGCCACGGTCGCAGCCGCGGGACCA
GCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGGTCTCGGGCGACCTCTCCTACTTA
CGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAGCCTCCTGGGAGGG
CCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGCTCG
GACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGAC
CGGGCTTCGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAG
GTGACTAACAGCAACTCTGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGG
CCGGCGGGCCCCGAGCTGCAGACCCCGCCGGNGAAAGATGGAGCAGTGGAGGACGAAGGA
AGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGNAGACCGAGAGCCGCTCTGGGCGAG
CCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGNGAAACTATTCGNACTC
AGAGGAAAGAGACGACGACGACGTGGCCTTCAGCAGACAGGTATTAAAGGACGACTNCCT
TTTCCGGNCATCGNCCAGACGAN
>OriGene 3' read for NM_014319 unedited
ATTTGGCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAAAACTA
ATAAATTTTTAATTAGAATTTTTTCCTTTTTTTAACAGGACAAGTAACAGATTACATCAA
ACTTCAGAACTTCTCAAATACCTAGTTATTATACACATTCCCATCTGTCTTGCAAGGAGG
GATCTTGGTCGGCTTAACAGTTTCAGGTAAAATAAGCTGCATAATAGTATATATTACAAT
AATAAATGTACAATGTTTACATGAAATATGTTTTAGAAGCAAGAAAAAGATGCATCTGCA
TCAAAGGCGACCTTTTAAAATGCCACAACTATTTTTTACATTCACTCTTGCAACAGGTTA
CAAGATGGGGGTCACTAAGCTCAGATAAATCACTATGATGCACATAAAATACAGGAAGAC
TGGTTACTTTATATATTATATATATTTATATTTTCACACGTTAGCATCTGAGACATAAAC
TGAGAATTTAAAATTTATTCTCTTCAGATAACAAATCATTTTTTATTCTTCATAAACAAG
GTGCCCACAAAAGCACACAGGTAAGGCACAATAGGGCTTTCTGTTACAGAATAGTGTGCA
TCATGTTTACTTAACCAGAAAACGTGCATTATCTGTGCAAGTTCATGCTGCAAACCCAAC
GCATACAATAAAACAAAAAGGAAACTGAGCTTTAAATACAACCAAAATTTGTACAATTCC
AGCTGTTTCACATATTCATTTGGACTGANCATTATATATCAACAGGTTATTTTTAAAAGC
CAATTGTAGATTACAAGTAAAATTTACTCAGCAACTATTTTTATCCTGAATCATAAACAG
TNNATAAGTAAGTTTACTGAAACAAATTAATACTTGAGCACANAGCACAGTCCATCGTGC
ATTTACTATATTGCAAACTTTATGACCCCAAATGCAAGTTAATTTGTCAAAACACATTTG
CTGAA
Restriction Sites NotI-NotI     
ACCN NM_014319
Insert Size 4140 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014319.3, NP_055134.2
RefSeq Size 4785 bp
RefSeq ORF 2736 bp
Locus ID 23592
UniProt ID Q9Y2U8
Domains LEM
Protein Families Transmembrane
Gene Summary This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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