EHD3 (NM_014600) Human Untagged Clone

CAT#: SC114949

EHD3 (untagged)-Human EH-domain containing 3 (EHD3)



  "NM_014600" in other vectors (6)

CNY 3,992.00

CNY 8,550.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


EHD3 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PAST3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC114949 sequence for NM_014600 edited (data generated by NextGen Sequencing)
ATGTTCAGCTGGCTGGGTACGGACGACCGCCGGAGGAAGGACCCCGAGGTTTTCCAGACG
GTGAGTGAGGGGCTCAAGAAACTCTACAAGAGCAAGCTGCTGCCCTTGGAAGAGCATTAC
CGCTTCCACGAGTTCCACTCGCCCGCCCTGGAGGATGCCGACTTCGACAACAAGCCCATG
GTTCTGCTGGTGGGCCAGTACTCCACTGGGAAGACCACCTTCATCAGGTACCTGCTGGAA
CAGGACTTCCCAGGCATGAGGATTGGGCCTGAGCCCACCACAGACTCCTTCATTGCGGTG
ATGCAGGGAGACATGGAGGGGATCATCCCTGGGAACGCCCTGGTGGTGGATCCCAAGAAA
CCCTTCAGGAAACTCAACGCCTTTGGCAACGCCTTCTTGAACAGGTTCGTGTGTGCCCAG
CTACCTAACCCTGTGCTGGAGAGCATCAGCGTCATCGACACACCAGGGATCCTCTCTGGG
GAGAAGCAGAGGATCAGCCGGGGGTATGACTTTGCAGCTGTCCTTGAGTGGTTTGCCGAG
CGGGTTGACCGCATCATTCTGCTCTTCGATGCCCACAAACTGGACATCTCTGATGAGTTC
TCAGAAGTCATCAAAGCCCTCAAGAACCACGAGGACAAGATGCGAGTGGTGCTGAACAAA
GCTGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACGGGGCCCTCATGTGGTCCTTG
GGGAAGATCGTGAACACCCCAGAGGTGATCCGGGTCTACATCGGCTCCTTCTGGTCCCAC
CCCCTCCTCATCCCTGACAACCGGAAGCTCTTTGAGGCTGAGGAACAGGACCTATTCAGG
GACATCCAGAGTCTGCCCCGAAATGCTGCCCTGCGCAAGCTCAACGACCTCATCAAAAGG
GCCAGGCTGGCCAAGGTCCACGCCTACATCATCAGCTCTCTGAAGAAGGAGATGCCCTCG
GTGTTCGGGAAGGACAACAAGAAGAAGGAGCTGGTCAACAACCTGGCCGAGATCTATGGC
CGGATCGAGCGGGAGCACCAGATCTCACCTGGGGACTTCCCCAATCTGAAGAGGATGCAG
GACCAGCTGCAGGCCCAGGACTTTAGCAAGTTCCAGCCGCTGAAGAGCAAGCTGCTGGAG
GTAGTGGACGACATGCTGGCCCATGACATTGCCCAGCTCATGGTGCTAGTGCGCCAGGAG
GAGTCACAGCGGCCCATCCAGATGGTGAAGGGCGGAGCGTTCGAGGGCACCCTGCACGGC
CCCTTTGGGCATGGCTATGGGGAGGGGGCTGGAGAAGGTATCGATGATGCTGAGTGGGTG
GTGGCCAGGGACAAGCCCATGTACGACGAGATCTTCTACACCCTGTCACCGGTGGATGGC
AAGATCACAGGCGCTAATGCCAAGAAGGAGATGGTGCGCTCCAAGCTGCCCAACAGTGTG
CTGGGCAAGATCTGGAAGCTGGCCGACATTGACAAGGATGGCATGCTGGACGACGACGAG
TTTGCACTGGCCAACCACCTCATCAAAGTCAAGCTGGAGGGGCACGAGCTGCCCAACGAG
CTGCCTGCCCACCTCCTGCCCCCGTCCAAGAGGAAAGTTGCCGAGTGA

Clone variation with respect to NM_014600.2
>OriGene 5' read for NM_014600 unedited
NNNNCCGTTCAAAATATTTGTNATAACGAACCCACTTTAGTNGGCGGCCGCGNACTTCGG
CACGAGTCCCGGCAGGAAGCGGAACTCTAAGACATGCCCTCTGCTGCACGCCAGGCAGAA
GCAAGGCGAAGGGCCTGGGGGCCGACCGGGGACCCCGGCATCTTGGCAGTTTCCCTTTGC
AGGTTCAAACTTTTAATTTGCCAAGCATTCACCCCTCCTCCTCAAGCCCAAATTACATTT
ACTCCTCCCTTCCGGCCTTGGGCTTGCTTGATGCAGCAGCGGCTGGGCTTTGGTCCCAGG
AAGCAGGGAGAGTGCGCTCCCGGCCCTTCTAAGCCCGTGCCCGGGCCATGGTGCNGNCTG
AGCCCCGCGCTTGGGTGAGGCGGCGGCGCGNCTCGGACCCCGCGGACCGGTCCTACGGGA
CATCTTCCCCTGAGGAGGAGTCTTCCCCTGGGGCTTGCGTGCCGGGGCCGAGCGGCGGCC
GCGATGTTCAGCTGGCTGGGTACGGACGAACCGCCGAAGAAGGAACCCNNAGNTTTCCAG
ACNGTGAGTGAGGGGCTTAAGAAACTCTACCAAACAAGCTGCTTCCCTTGGAAGAGCATT
ACCGCTTCACGAATTCCACTCGCCCGCCCTTGAAGATGCCCACTTCCACAACAGCCCATG
GGTCTTCTTGTGGGCCAGTACTCCACTTGNAAGACCCCTTTATCAAGTACCTGCTGGAAA
CAGACTTTCCAGCATAAAGATTTGGCCTGAGCCCACCACAGACTCCTTATTTTGCGTAAT
GCAGGGAACATTGNAGGGGATCATCCCTTGGAAGCTCCTGTGGGTGGATCCCAAAAAACC
CTTCAAGAAACTCAACGCCTTTTGCACGCCTTTTGAAACAAGTTCGTGTGTGCCCCACTA
CCTAAACTTGTTCTGGAAAACATTAACNNNTTCAACCAACAAGGATA
>OriGene 3' read for NM_014600 unedited
ACGTGGAAAACGAGGCANCGCTGCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTACCAAA
NAATAAATGTACTGTATTAACATGAAGACTAATGACAAATGCACTGCAGTAGTAAGCACG
TCATAGATGCATAGAATATTCTCTATATAGTCTGAATATGGATATAAAATAAGTTATACT
CATTTTGTTTTCCATCACAGTAGGAGCATATCTCCCTCAGTGATTGGNTCAGTGGCCATG
AAGCAAGCCAGGGGAGAGACCACAGAAGAGAATGTAGGGCATTGAGTACAGTGGGGATTT
GCCAAGGACACTGCAGAGTCCCTGGGGACCCTCTGGGGAACAAGGCCCCAAACCTCTCAA
GTTAGCCTTCTGAGAATAGTATGAGGTCCCACTTGACAAGAGACAGTGGCTGCATCTGAG
CTGGGATGATTCAGGCAACTGGGTGGGAGGCAGAGCAGGGACAGGCACGAACAGCATCCA
GCAGGCTAGCCCACTCTCCTGCTGGTGCTGTTGGATGTCTATGATGCTGGGAATATGGGG
CGCATAGCCAGAAGGCACGGTTTGCTTCTGTGACGTGACAGATGGCCAGACTGATGGCCC
CCCAAATGCTGGCCGGCCCTCCTCCCTTGCNCTCCTGTCCTGGGAGGCTGCAGGGGGCCT
GCCTACTTTGCTTGGTCTCCCTAGTTTGGCTTGGGTTTTAATGCTGGGATCCTGCCTTCT
TCTTGCGCAGACAAGTGGGTGAGGGTCTGGGGCCCTGGCTTTGAATCCTTTCTCCTCCGT
GACTTCTGGGAGGTTTGTAGGGTGTTCTTACCCTTCGGGGGTCCTCGNTCCCCCTTCTGC
CTTTGGCGAGCTCATGCCTTCCTCCCGGGGGCTTGGGGGTGCCCCTTTGTCTTTGGTCTT
CCCTGCCTACCCGCTTCTTGTTGCCCGGAGGGCTGGATAAATTGAAAGAAAAAAAAAT
Restriction Sites NotI-NotI     
ACCN NM_014600
Insert Size 3680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014600.1, NP_055415.1
RefSeq Size 3583 bp
RefSeq ORF 1608 bp
Locus ID 30845
UniProt ID Q9NZN3
Domains EH, EFh
Protein Pathways Endocytosis
Gene Summary ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (PubMed:25686250). In vitro causes tubulation of endocytic membranes (PubMed:24019528). Binding to phosphatidic acid induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology (PubMed:16251358, PubMed:17233914, PubMed:19139087, PubMed:23781025). Involved in the recycling of internalized D1 dopamine receptor (PubMed:21791287). Plays a role in cardiac protein trafficking probably implicating ANK2 (PubMed:20489164). Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (By similarity). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (By similarity). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance (PubMed:23781025). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1 (PubMed:25686250).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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