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PPM1F (NM_014634) Human Untagged Clone
CAT＃: SC114924
PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114924 sequence for NM_014634 edited (data generated by NextGen Sequencing)
ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC GGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAGCCTTCCGGCGCACCGACCAG ATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCAGAGCGGCACCACAGGTGTGTGTGCG CTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTA CAGCAGGGACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAGAAG GCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCACATGGACTGCTGGAGAGTCAACGGG ACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTTCCAGAAGCCCTACGTGTCTGGGGAG GCCGATGCAGCTTCCCGGGCGCTGACGGGCTCCGAGGACTACCTGCTGCTTGCCTGTGAT GGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGTTGGCCTGGTCCAGAGCCACCTGACC AGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCGGGAGCGG GGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCCCCAAGAGCTGCTG GAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAGAAGGGAGGAGGCAGGACTTGCCC TCCAGCCTTCCAGAACCTGAGACCCAGGCTCCACCAAGAAGCTAG Clone variation with respect to NM_014634.3 >OriGene 5' read for NM_014634 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCGCGGAGACCGGG GGTGAAGCCTGGAGACCCTCTTGCCCTGGCCTAGCTGCAGGCCCCCGGGATGCTTTGGGC ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC GGNAGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAACCTTCCGGCGCACCGACCAG ATGNNTCTCAGGGAAGCCAAGCGAGAGCGGCTGCAGAGCGCACCACAGTGGTGTGTGCGC TCATTGGCAGAGCGACCNTGCACGTCGCCTGGCTCGGGGATTCCCAGTCC >OriGene 3' read for NM_014634 unedited
CCGCGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCTTGAAAAAAGCTTTAA AAAAGTAAATACTTAAGTTTTCTAAAGAATAAAATAAATGCTAAGCTCTGCTTAAATTAT ATGACACAACAGGTCATCTTGGCACTGATAAAACAAAGAGAAAACCTGGAATACTGCTTC GGAATTAGACCTCCCTGGAAAAAAACAAACACACCAACAAAAGACACATGTGCGTCGCCG GTTCCGGGCTCAGGGAAGAGAGCAGCCCGGAAGGCCCTGGTGGGCCCAAGCACATCATGG CTGCCCTGGAGTGGGGGCAGGGGGGTGGCTGCTACAGAACAGACCCCTGGCTCTGCTGCG CTCTCCTCTTTGGGACACTGGTGTGGCCGACCAGGACTGGCTGGGCATATCTGTAAATAT GAACCCACCTGGCCCTGGCCGCAGAGAGGAGCAATGGGAAACTGGGGCTGGCCACTCCGC TGCTGGGCCTGACCCAGGGGGTGCTTCAAGAGTTTGGCTCCTGGGTCCGCCCCAGGGTTC CACGTGCACAACCATGCTGCCCCATGCACACCAGCGTCTTTGGTTTGGCTGCCGTTGCGC ACCTATGACCTCTGGACCCACCCCGGGCCTACTAGGACCTGGGAGCAAGAGCCCCTCCAT CTTTTCTTTGGCGAGGCCTCCTAACCTGCCTTCCCCCTCCTGCCGCCACCAGTGCAGTTC ACAGCCACAAGAAGGCCTGTGGGGGGTGCTCCAACCGCCTTGGGGCGCTTGGGAAACCCT GCGGGGCGGGCCCCTGTCCACTGCCTGCACCTGTGGCCCCGAGGCCTCTGAGGAACACGC CACCGATGCAGCATGTCAGCCCCCCCCCTTCACCTTTTACCCTTGTTTTACCCCCCTTCA CTATTCAAATCCCTTCCTCCCCTCTTCTATCTCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014634 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014634.2, NP_055449.1 |
| RefSeq Size | 5169 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 9647 |
| UniProt ID | P49593 |
| Domains | PP2C |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210043 | PPM1F (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) |
CNY 8,200.00 |
|
| RC210043L1 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC210043L2 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged |
CNY 13,680.00 |
|
| RC210043L3 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), Myc-DDK-tagged |
CNY 13,680.00 |
|
| RC210043L4 | Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F), mGFP tagged |
CNY 13,680.00 |
|
| RG210043 | PPM1F (tGFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) |
CNY 9,800.00 |
|
| SC320526 | PPM1F (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1F (PPM1F) |
CNY 3,656.00 |
