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SAPS2 (PPP6R2) (NM_014678) Human Untagged Clone
CAT＃: SC114906
PPP6R2 (untagged)-Human protein phosphatase 6, regulatory subunit 2 (PPP6R2)
CN¥ 10,248.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0685; PP6R2; SAP190; SAPS2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114906 sequence for NM_014678 edited (data generated by NextGen Sequencing)
ATGTTCTGGAAGTTTGACTTGAACACCACGTCCCATGTTGACAAGCTGCTGGACAAGGAG CATGTGACGCTGCAGGAGTTAATGGATGAAGATGACATCTTGCAGGAGTGTAAGGCTCAG AACCAGAAGCTGCTGGACTTCCTGTGCAGGCAGCAGTGCATGGAGGAGCTGGTGAGCCTC ATCACACAGGATCCGCCCCTGGACATGGAGGAGAAGGTCCGCTTCAAATATCCAAACACA GCCTGTGAGCTTCTGACTTGTGATGTGCCGCAGATCAGCGACCGCCTCGGTGGGGACGAG AGCCTGCTGAGCCTCCTGTACGACTTCTTGGACCATGAGCCGCCTCTCAATCCTCTGCTC GCCAGTTTTTTCAGCAAGACCATTGGCAATCTCATTGCAAGAAAAACCGAACAGGTGATT ACGTTTTTGAAGAAGAAGGACAAGTTCATCAGCCTGGTGTTGAAGCACATCGGCACCTCA GCGCTTATGGACCTGCTGCTGCGCCTGGTCAGCTGTGTGGAGCCAGCCGGGCTCCGGCAG GACGTCCTGCACTGGCTGAATGAAGAGAAGGTCATCCAGAGGCTTGTGGAGTTGATCCAC CCGAGCCAGGATGAAGATAGGCAGTCAAATGCTTCTCAGACTCTCTGTGACATAGTTAGG CTGGGCAGAGACCAGGGCAGTCAGCTGCAAGAGGCTCTGGAGCCAGACCCGCTCCTCACA GCGCTGGAGTCGCAGGACTGTGTGGAGCAGCTTCTGAAGAACATGTTTGATGGAGACCGG ACGGAGAGCTGCCTCGTCAGTGGGACTCAGGTGTTACTCACCTTGCTGGAAACCAGGCGG GTTGGGACAGAGGGCTTGGTGGACTCCTTTTCTCAGGGACTGGAAAGGTCATACGCTGTC AGCAGCAGCGTACTACACGGCATCGAGCCTCGGCTGAAGGACTTCCACCAGCTCCTGCTC AACCCGCCCAAGNNNNNAGCGATCCTGACCACCATTGGTGTGCTGGAGGAGCCCCTGGGG AATGCCCGTCTGCATGGCGCCCGCCTCATGGCAGCACTGCTGCACACAAACACACCCAGC ATCAACCAGGAGCTCTGCCGGCTCAACACGATGGACTTACTGCTGGACTTGTTCTTTAAG TACACCTGGAATAACTTTTTGCACTTCCAAGTGGAACTATGCATAGCCGCTATTCTCTCC CACGCTGCCCGTGAGGAGAGGACAGAAGCCAGCGGATCCGAGAGCAGGGTGGAGCCTCCG CATGAGAACGGGAACCGGAGCCTGGAGACTCCCCAGCCGGCCGCCAGCCTCCCTGACAAC ACAATGGTGACCCACCTGTTCCAGAAGTGCTGCCTGGTGCAGAGGATCCTGGAGGCCTGG GAAGCCAACGACCACACGCAGGCAGCGGGTGGCATGAGACGTGGGAACATGGGCCACCTC ACACGGATCGCCAACGCGGTGGTGCAGAACCTGGAGCGGGGCCCTGTGCAGACGCACATC AGCGAGGTCATCCGAGGGCTCCCTGCGGACTGCCGTGGCCGCTGGGAGAGCTTCGTGGAG GAGACGCTGACGGAGACGAACCGCAGGAACACTGTGGACCTGGCCTTCTCTGACTACCAG ATCCAGCAGATGACAGCCAACTTCGTGGATCAGTTTGGCTTCAATGATGAGGAGTTTGCC GACCAGGACGACAACATCAATGCCCCGTTTGACAGGATCGCAGAGATCAACTTCAACATC GACGCTGACGAGGACAGTCCCAGCGCAGCTCTGTTTGAGGCCTGCTGCAGTGACCGCATC CAGCCCTTTGATGATGATGAGGACGAGGACATCTGGGAGGACAGTGACACTCGCTGTGCT GCCCGGGTGATGGCCAGACCCAGGTTTGGAGCCCCCCATGCTTCAGAGAGTTGCTCAAAG AATGGCCCAGAGCGTGGAGGCCAGGATGGGAAGGCGAGCTTGGAAGCACACAGAGATGCA CCTGGGGCAGGTGCCCCACCGGCCCCCGGGAAGAAGGAAGCCCCCCCTGTGGAGGGTGAC TCAGAAGGCGCCATGTGGACGGCAGTGTTTGATGAGCCAGCGAACTCAACGCCCACAGCC CCAGGAGTGGTGAAGGACGTGGGTTCCAGTGTGTGGGCAGCTGGCACCTCAGCTCCAGAG GAGAAAGGCTGGGCCAAGTTCACTGACTTCCAACCTTTCTGCTGCTCCGAGTCAGGGCCC AGGTGCAGCTCTCCGGTGGACACAGAATGCAGCCATGCTGAGGGCAGCCGGAGCCAAGGC CCTGAGAAAGCCTTCAGCCCGGCTTCTCCATGTGCCTGGAACGTGTGTGTCACCAGGAAG GCCCCCCTGCTGGCCTCTGACAGTAGCTCCTCTGGGGGCTCCCACAGCGAGGATGGCGAC CAGAAGGCAGCGAGTGCCATGGATGCGGTGAGCAGGGGTCCCGGCCGGGAGGCCCCCCCG CTGCCCACAGTGGCCAGGACAGAGGAGGCTGTCGGCAGGGTCGGGTGTGCTGACAGCCGG CTGTTAAGCCCTGCCTGCCCCGCGCCAAAGGAAGTGACTGCTGCCCCAGCCGTGGCTGTG CCCCCCGAGGCTACTGTGGCCATCACCACAGCACTGAGCAAGGCTGGCCCCGCCATACCC ACCCCAGCAGTCTCTTCTGCACTGGCCGTGGCGGTCCCCCTAGGGCCCATCATGGCAGTC ACAGCAGCCCCAGCCATGGTGGCCACCCTGGGGACAGTGACAAAGGACGGGAAGACAGAT GCCCCGCCAGAAGGAGCTGCCTTAAATGGCCCAGTGTGA Clone variation with respect to NM_014678.4 973 a=>n;974 a=>n;975 g=>n;976 a=>n;977 a=>n;2022 g=>c;2114 g=>a >OriGene 5' read for NM_014678 unedited
GTGTCANGATATGNATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGAGCGATC GGAGTGCCGCCCGCGGCCCCGAGTCGGTCTCGAGCCGCCGGCCGGCCGTGCCGGTGTCCG TAGGCGCTGCGCCCTCGGCCGGGCCCATGTGTGTGCGGCCCGCCCGAGGCCGCCCGGGCT TTGCCTCCACCAGCGCCCTGGCCTCCGCTCGGGCCTCCACACGGGCCTCCGAAGAGCTGC CGCGACGCCCGGCCCGCAGGGCAGGTAAAGAGATTATAAATCTTCCACTGAATGAAAAAA ATTTTCTTAAAGCTGCATATACTCCAAGAAAAAAACCACAAATGTTTTTCTGTTTTGCCT GAATACATGATTTAAACAAGAGATTTCCACAGAAGCTCTGCGGCCGTCACGATGTTCTGG AAGTTTGACTTGAACACCACGTCCCATGTTGACAAGCTGCTGGACAAGGAGCATGTGACG CTGCAGGAGTTAATGGATGAAGATGACATCTTGCAGGAGTGTAAGGCTCAGAACCAGAAG CTGCTGGACTTCCTGTGCAGGCAGCAGTGCATGGAGGAGCTGGTGAGCCTCATCACACAG GATCCGCCCCTGGACATGGAGGAGAAGGTCCGCTTCAAATATCCAAACACAGCCTGTGAG CTTCTGACTTGTGATGTGCCGCAGATCAGCGACCGCCTCGGTGGGGACGAGAGCCTGCTG AGCCTCCTGTACGACTTCTTGGACCATGAGCCGCCTCTCAATCCTCTGCTCGCCAGTTTT TTCAGCAAGACCATTGGCAATCTCATTGCAAGAAACACCGAACAGGTGATTACGTTTTTG AAGAAGAGGACAAGTTCATCAGCCTGGTGTTGAAGCACATCGGCACCTCAGCGCTTATGG ACCTGCTGCTGCGCCTGGTCAGCTGTGG >OriGene 3' read for NM_014678 unedited
NGCATCCATAGGGTTTAAAACCAAATTCATNAATTAAAAAAAACCCATGCATCACCAGGT AGTTTTCTCGATTAAGGAGGCAGCCTGACCAGGGTGGGCCGTGGCCGGGCGGCAGCAGCA TCACACTGGGCCATTTAAGGCAGCTCCTTCTGGCGGGGCATCTGTCTTCCCGTCCTTTGT CACTGTCCCCAGGGTGGCCACCATGGCTGGGGCTGCTGTGACTGCCATGATGGGCCCTAG GGGGACCGCCACGGCCAGTGCACAAGAGACTGCTGGGGTGGGTATGGCGGGGCCAGCCTT GCTCAGTGCTGTGGTGATGGCCACAGTAGCCTCGGGGGGCACAGCCACGGCTGGGGCAGC AGTCACTTCCTTTGGCGCGGGGCAGGCAGGGCTTAACAGCCGGCTGTCAGCACACCCGAC CCTGCCGACAGCCTCCTCTGTCCTGGCCACTGTGGGCAGCGGGGGGGCCTCCCGGCCGGG ACCCCTGCTCACCGCATCCATGGCACTCGCTGCCTTCTGGTCGCCATCCTCGCTGTGGGA GCCCCCACAGGAGCTCCTGTCCGAGGCCCGCACGGGGGCCTTCCTGGTGACACACACGTT CCAGGCCCATGGTGTAGCCGGGCCTGAAGCTTTCTCATGGCCTTGGCTCCCGGCTGCCCC TCAGCTTGGCCGCCTTCTGTGGCCCCCGGGGAGCTGCCCCTGGGCCCTGTCTCGGGGCCG CTGCAAGGTTGGAAGTTACTGAACTTTGGCCCCACCTTTTCCCTCTGGCGCTGGGGGGGC CCCTGNCCACACCCGGGAACGCCCGTTCTTCCCCCTCCTTGGGGCTGTGGGCCGTTCTGT CCCTCGTGCTTTTTAACATTGTCGCCCCCCTGGCGCCTTTTTACTCCTCCCCTCCGGCGG GGGCTTCTTTCATCCAGAGACAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014678 |
| Insert Size | 3850 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014678.3, NP_055493.2 |
| RefSeq Size | 4004 bp |
| RefSeq ORF | 2799 bp |
| Locus ID | 9701 |
| UniProt ID | O75170 |
| Domains | SAPS |
| Gene Summary | The protein encoded by this gene is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment. [provided by RefSeq, May 2017] Transcript Variant: This variant (3) lacks an exon in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207527 | PPP6R2 (Myc-DDK-tagged)-Human protein phosphatase 6, regulatory subunit 2 (PPP6R2) |
CN¥ 10,248.00 |
|
| RC207527L3 | Lenti ORF clone of Human protein phosphatase 6, regulatory subunit 2 (PPP6R2), Myc-DDK-tagged |
CN¥ 9,030.00 |
|
| RC207527L4 | Lenti ORF clone of Human protein phosphatase 6, regulatory subunit 2 (PPP6R2), mGFP tagged |
CN¥ 9,030.00 |
|
| RG207527 | PPP6R2 (tGFP-tagged) - Human protein phosphatase 6, regulatory subunit 2 (PPP6R2) |
CN¥ 11,848.00 |
|
| SC324353 | PPP6R2 (untagged)-Human protein phosphatase 6, regulatory subunit 2 (PPP6R2) |
CN¥ 10,248.00 |
