EIF4A3 (NM_014740) Human Untagged Clone

CAT#: SC114843

EIF4A3 (untagged)-Human eukaryotic translation initiation factor 4A3 (EIF4A3)



  "NM_014740" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


EIF4A3 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DDX48; eIF-4A-III; eIF4A-III; eIF4AIII; Fal1; MUK34; NMP265; NUK34; RCPS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC114843 sequence for NM_014740 edited (data generated by NextGen Sequencing)
ATGGCGACCACGGCCACGATGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTCAAAGAG
GAAGACATGACTAAAGTGGAATTCGAGACCAGCGAGGAGGTGGATGTGACCCCCACGTTC
GACACCATGGGCCTGCGGGAGGACCTGCTGCGGGGCATCTACGCTTACGGTTTTGAAAAA
CCATCAGCAATCCAGCAACGAGCAATCAAGCAGATCATCAAAGGGAGAGATGTCATCGCA
CAGTCTCAGTCCGGCACAGGAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAGTGTTTG
GATATTCAGGTTCGTGAAACTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTGGCTGTG
CAGATCCAGAAGGGGCTGCTTGCTCTCGGTGACTACATGAATGTCCAGTGCCATGCCTGC
ATTGGAGGCACCAATGTTGGCGAGGACATCAGGAAGCTGGATTACGGACAGCATGTTGTC
GCGGGCACTCCAGGGCGTGTTTTTGATATGATTCGTCGCAGAAGCCTAAGGACACGTGCT
ATCAAAATGTTGGTTTTGGATGAAGCTGATGAAATGTTGAATAAAGGTTTCAAAGAGCAG
ATTTACGATGTATACAGGTACCTGCCTCCAGCCACACAGGTGGTTCTCATCAGTGCCACG
CTGCCACACGAGATTCTGGAGATGACCAACAAGTTCATGACCGACCCAATCCGCATCTTG
GTGAAACGTGATGAATTGACTCTGGAAGGCATCAAGCAATTTTTCGTGGCAGTGGAGAGG
GAAGAGTGGAAATTTGACACTCTGTGTGACCTCTACGACACACTGACCATCACTCAGGCG
GTCATCTTCTGCAACACCAAAAGAAAGGTGGACTGGCTGACGGAGAAAATGAGGGAAGCC
AACTTCACTGTATCCTCAATGCATGGAGACATGCCCCAGAAAGAGCGGGAGTCCATCATG
AAGGAGTTCCGGTCGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCAGGGGG
TTGGATGTCCCTCAGGTGTCCCTCATCATTAACTATGATCTCCCTAATAACAGAGAATTG
TACATACACAGAATTGGGAGATCAGGTCGATACGGCCGGAAGGGTGTGGCCATTAACTTT
GTAAAGAATGACGACATCCGCATCCTCAGAGATATCGAGCAGTACTATTCCACTCAGATT
GATGAGATGCCGATGAACGTTGCTGATCTTATCTGA

Clone variation with respect to NM_014740.3
>OriGene 5' read for NM_014740 unedited
GTTACCATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCACAGCGAG
GTCGGCAGCGGCACAGCGAGGTCGGCAGCGGCACAGCGAGGTCGGCAGCGGCAGCGAGGT
CGGCAGCGGCGCGCGCTGTGCTCTTCCGCGGACTCTGAATCATGGCGACCACGGCCACGA
TGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTCAAAGAGGAAGACATGACTAAAGTGG
GATTCGAGACCAGCGAGGAGGTGGATGTGACCCCCACGTTCGACACCATGGGCCTGCGGG
AGGACCTGCTGCGGGGCATCTACGCTTACGGTTTTGAAAAACCATCAGCAATCCAGCAAC
GAGCAATCAAGCAGATCATCAAAGGGAGAGATGTCATCGCACAGTCTCAGTCCGGCACAG
GAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAGTGTTTGGATATTCAGGTTCGTGAAA
CTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTGGCTGTGCAGATCCAGAAGGGGCTGC
TTGCTCTCGGTGACTACATGAATGTCCAGTGCCATGCCTGCATTGGAGGCACCAATGTTG
GCGAGGACATCAGGAAGCTGGATTACGGACAGCATGTTGTCGCGGGCACTCCAGGGCGTG
TTTTTGATATGATTCGTCGCAGAAGCCTAAGGACACGTGCTATCAAAATGTTGGTTTTGG
ATGAAGCTGATGAAACGCTGAATAAAGGTTTCAAAGAGCAGATTTACGATGTATACAGGT
ACCTGCCTTCAGCCACACAGGTGGTTCTCATCAGTGGCACGCTGGCACACCAGATTCTGG
AGATGACCAACAAGTTCATGACCGACCCCATCCGCATTTTGGTGAAAACGGGATGAATTG
ACCTCTGGAAGTCTCCAGCCAATTTTTCG
>OriGene 3' read for NM_014740 unedited
GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGAAAAGTTTTAAA
TTTTTAATGAAAAAGACTTAGAACAATGTATTATTTACATGTAAATAAGAAAAGAGAGCA
GAATCCCCATATCCTCTTCAAAGGAAGGGAGACGGCAGGCCATTTATGAGAAGAAAGTCC
ATATAAACCCCATTAAGTAGAATCTGGATCTAAATACTTCCAAACAGGAGTACACAGCAG
GTGAACAGTCTCCCTCATCCCACTGATCTGCTGCTTCAGATAAGATCAGCAACGTTCATC
GGCATCTCATCAATCTGAGTGGAATAGTACTGCTCGATATCTCTGAGGATGCGGATGTCG
TCATTCTTTACAAAGTTAATGGCCACACCCTTCCGGCCGTATCGACCTGATCTCCCAATT
CTGTGTATGTACAATTCTCTGTTATTAGGGAGATCATAGTTAATGATGAGGGACACCTGA
GGGACATCCAACCCCCTGGCCCAGACATCTGTAGAAATAAGCACTCGGCTGGCGCCCGAC
CGGAACTCCTTCATGATGGACTCCCGCTCTTTCTGGGGCATGTCTCCATGCATTGAGGAT
ACAGTGAAGTTGGGCTTCCCTCATTTTCTCCGTCAGCCAGTCCACCTTTCTTTTGGTGTT
GCANAAGATGACCGCCTGAGTGATGGGCAGTGTGTCGTACAGGGCACACAGAGTGTCAAA
TTTTCACCTCTTCCTTTTCACTGCCACGAAAAATTGCTTGATGCCTCCAGAGTCATTCAT
CACGTTTCACCCAGAGCCGGATTGGGTCGGCATGACCTTGTTGGTATCTCCAGAATTCGG
GGGGACCGTGCCCTGAGAAAACCCCTGGGGGGTGGAGCAGGCCTGATACATGGAAACGGT
TTTGAACCTTTTCAATTTATCACT
Restriction Sites NotI-NotI     
ACCN NM_014740
Insert Size 1740 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014740.2, NP_055555.1
RefSeq Size 1702 bp
RefSeq ORF 1236 bp
Locus ID 9775
UniProt ID P38919
Domains DEAD, helicase_C
Protein Families Druggable Genome
Protein Pathways Spliceosome
Gene Summary This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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