EIF4A3 (NM_014740) Human Untagged Clone
CAT#: SC114843
EIF4A3 (untagged)-Human eukaryotic translation initiation factor 4A3 (EIF4A3)
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DDX48; eIF-4A-III; eIF4A-III; eIF4AIII; Fal1; MUK34; NMP265; NUK34; RCPS |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC114843 sequence for NM_014740 edited (data generated by NextGen Sequencing)
ATGGCGACCACGGCCACGATGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTCAAAGAG GAAGACATGACTAAAGTGGAATTCGAGACCAGCGAGGAGGTGGATGTGACCCCCACGTTC GACACCATGGGCCTGCGGGAGGACCTGCTGCGGGGCATCTACGCTTACGGTTTTGAAAAA CCATCAGCAATCCAGCAACGAGCAATCAAGCAGATCATCAAAGGGAGAGATGTCATCGCA CAGTCTCAGTCCGGCACAGGAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAGTGTTTG GATATTCAGGTTCGTGAAACTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTGGCTGTG CAGATCCAGAAGGGGCTGCTTGCTCTCGGTGACTACATGAATGTCCAGTGCCATGCCTGC ATTGGAGGCACCAATGTTGGCGAGGACATCAGGAAGCTGGATTACGGACAGCATGTTGTC GCGGGCACTCCAGGGCGTGTTTTTGATATGATTCGTCGCAGAAGCCTAAGGACACGTGCT ATCAAAATGTTGGTTTTGGATGAAGCTGATGAAATGTTGAATAAAGGTTTCAAAGAGCAG ATTTACGATGTATACAGGTACCTGCCTCCAGCCACACAGGTGGTTCTCATCAGTGCCACG CTGCCACACGAGATTCTGGAGATGACCAACAAGTTCATGACCGACCCAATCCGCATCTTG GTGAAACGTGATGAATTGACTCTGGAAGGCATCAAGCAATTTTTCGTGGCAGTGGAGAGG GAAGAGTGGAAATTTGACACTCTGTGTGACCTCTACGACACACTGACCATCACTCAGGCG GTCATCTTCTGCAACACCAAAAGAAAGGTGGACTGGCTGACGGAGAAAATGAGGGAAGCC AACTTCACTGTATCCTCAATGCATGGAGACATGCCCCAGAAAGAGCGGGAGTCCATCATG AAGGAGTTCCGGTCGGGCGCCAGCCGAGTGCTTATTTCTACAGATGTCTGGGCCAGGGGG TTGGATGTCCCTCAGGTGTCCCTCATCATTAACTATGATCTCCCTAATAACAGAGAATTG TACATACACAGAATTGGGAGATCAGGTCGATACGGCCGGAAGGGTGTGGCCATTAACTTT GTAAAGAATGACGACATCCGCATCCTCAGAGATATCGAGCAGTACTATTCCACTCAGATT GATGAGATGCCGATGAACGTTGCTGATCTTATCTGA Clone variation with respect to NM_014740.3 >OriGene 5' read for NM_014740 unedited
GTTACCATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCACAGCGAG GTCGGCAGCGGCACAGCGAGGTCGGCAGCGGCACAGCGAGGTCGGCAGCGGCAGCGAGGT CGGCAGCGGCGCGCGCTGTGCTCTTCCGCGGACTCTGAATCATGGCGACCACGGCCACGA TGGCGACCTCGGGCTCGGCGCGAAAGCGGCTGCTCAAAGAGGAAGACATGACTAAAGTGG GATTCGAGACCAGCGAGGAGGTGGATGTGACCCCCACGTTCGACACCATGGGCCTGCGGG AGGACCTGCTGCGGGGCATCTACGCTTACGGTTTTGAAAAACCATCAGCAATCCAGCAAC GAGCAATCAAGCAGATCATCAAAGGGAGAGATGTCATCGCACAGTCTCAGTCCGGCACAG GAAAAACAGCCACCTTCAGTATCTCAGTCCTCCAGTGTTTGGATATTCAGGTTCGTGAAA CTCAAGCTTTGATCTTGGCTCCCACAAGAGAGTTGGCTGTGCAGATCCAGAAGGGGCTGC TTGCTCTCGGTGACTACATGAATGTCCAGTGCCATGCCTGCATTGGAGGCACCAATGTTG GCGAGGACATCAGGAAGCTGGATTACGGACAGCATGTTGTCGCGGGCACTCCAGGGCGTG TTTTTGATATGATTCGTCGCAGAAGCCTAAGGACACGTGCTATCAAAATGTTGGTTTTGG ATGAAGCTGATGAAACGCTGAATAAAGGTTTCAAAGAGCAGATTTACGATGTATACAGGT ACCTGCCTTCAGCCACACAGGTGGTTCTCATCAGTGGCACGCTGGCACACCAGATTCTGG AGATGACCAACAAGTTCATGACCGACCCCATCCGCATTTTGGTGAAAACGGGATGAATTG ACCTCTGGAAGTCTCCAGCCAATTTTTCG >OriGene 3' read for NM_014740 unedited
GACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGGAAAAGTTTTAAA TTTTTAATGAAAAAGACTTAGAACAATGTATTATTTACATGTAAATAAGAAAAGAGAGCA GAATCCCCATATCCTCTTCAAAGGAAGGGAGACGGCAGGCCATTTATGAGAAGAAAGTCC ATATAAACCCCATTAAGTAGAATCTGGATCTAAATACTTCCAAACAGGAGTACACAGCAG GTGAACAGTCTCCCTCATCCCACTGATCTGCTGCTTCAGATAAGATCAGCAACGTTCATC GGCATCTCATCAATCTGAGTGGAATAGTACTGCTCGATATCTCTGAGGATGCGGATGTCG TCATTCTTTACAAAGTTAATGGCCACACCCTTCCGGCCGTATCGACCTGATCTCCCAATT CTGTGTATGTACAATTCTCTGTTATTAGGGAGATCATAGTTAATGATGAGGGACACCTGA GGGACATCCAACCCCCTGGCCCAGACATCTGTAGAAATAAGCACTCGGCTGGCGCCCGAC CGGAACTCCTTCATGATGGACTCCCGCTCTTTCTGGGGCATGTCTCCATGCATTGAGGAT ACAGTGAAGTTGGGCTTCCCTCATTTTCTCCGTCAGCCAGTCCACCTTTCTTTTGGTGTT GCANAAGATGACCGCCTGAGTGATGGGCAGTGTGTCGTACAGGGCACACAGAGTGTCAAA TTTTCACCTCTTCCTTTTCACTGCCACGAAAAATTGCTTGATGCCTCCAGAGTCATTCAT CACGTTTCACCCAGAGCCGGATTGGGTCGGCATGACCTTGTTGGTATCTCCAGAATTCGG GGGGACCGTGCCCTGAGAAAACCCCTGGGGGGTGGAGCAGGCCTGATACATGGAAACGGT TTTGAACCTTTTCAATTTATCACT |
Restriction Sites | NotI-NotI |
ACCN | NM_014740 |
Insert Size | 1740 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014740.2, NP_055555.1 |
RefSeq Size | 1702 bp |
RefSeq ORF | 1236 bp |
Locus ID | 9775 |
UniProt ID | P38919 |
Domains | DEAD, helicase_C |
Protein Families | Druggable Genome |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202567 | EIF4A3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4A3 (EIF4A3) |
CNY 3,656.00 |
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RC202567L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A3 (EIF4A3), Myc-DDK-tagged |
CNY 6,056.00 |
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RC202567L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A3 (EIF4A3), mGFP tagged |
CNY 5,890.00 |
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RC202567L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A3 (EIF4A3), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202567L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4A3 (EIF4A3), mGFP tagged |
CNY 5,890.00 |
|
RG202567 | EIF4A3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4A3 (EIF4A3) |
CNY 5,256.00 |