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Glutaminase (GLS) (NM_014905) Human Untagged Clone
CAT＃: SC114750
GLS (untagged)-Human glutaminase (GLS), nuclear gene encoding mitochondrial protein
CNY 7,360.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAD20; CASGID; DEE71; EIEE71; GAC; GAM; GDPAG; GLS1; KGA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114750 sequence for NM_014905 edited (data generated by NextGen Sequencing)
ATGATGCGGCTGCGAGGCTCGGGGATGCTGCGGGACCTGCTCCTGCGGTCGCCCGCCGGC GTGAGCGCGACTCTGCGGCGGGCACAGCCCTTGGTCACCCTGTGCCGGCGTCCCCGAGGC GGGGGNNNNNNNNNNGCGGGCCCGGCTGCCGCCGCGCGACTCCACCCGTGGTGGGGCGGG GGCGGCTGGCCGGCGGAGCCCCTCGCGCGGGGCCTGNCCAGCTCTCCTTCGGAGATCTTG CAGGAGCTGGGCAAGGGGAGCACGCATCCGCAGCCCGGGGTGTCGCCACCCGCTGCCCCG GCGGCGCCCGGCCCCAAGGACGGCCCCGGGGAGACGGACGCGTTTGGCAACAGCGAGGGC AAAGAGCTGGTGGCCTCAGGTGAAAATAAAATAAAACAGGGTCTGTTACCTAGCTTGGAA GATTTGCTGTTCTATACAATTGCTGAAGGACAAGAGAAAATACCTGTTCATAAATTTATT ACAGCACTCAAATCTACAGGATTGCGAACGTCTGATCCCAGGTTGAAAGAGTGTATGGAT ATGTTAAGATTAACTCTTCAAACAACATCAGATGGTGTCATGCTAGACAAAGATCTTTTT AAAAAATGTGTTCAGAGCAACATTGTTTTGTTGACACAAGCATTTAGAAGAAAGTTTGTG ATTCCTGACTTTATGTCTTTTACCTCACACATTGATGAGTTATATGAAAGTGCTAAAAAG CAGTCTGGAGGAAAGGTTGCAGATTATATTCCTCAACTGGCCAAATTCAGTCCCGATTTG TGGGGTGTGTCTGTTTGTACAGTAGATGGACAGAGGCATTCTACTGGAGATACCAAAGTT CCCTTCTGTCTTCAGTCCTGTGTAAAACCTTTGAAATATGCCATTGCTGTTAATGATCTT GGAACTGAATATGTGCATCGATATGTTGGAAAAGAGCCGAGTGGACTAAGATTCAACAAA CTATTTTTGAATGAAGATGATAAACCACATAATCCTATGGTAAATGCTGGAGCAATTGTT GTGACTTCACTAATAAAGCAAGGAGTAAATAATGCTGAAAAATTTGACTATGTCATGCAG TTTTTGAATAAGATGGCTGGTAATGAATATGTTGGATTCAGTAATGCAACGTTTCAGTCT GAAAGAGAAAGTGGAGATCGAAATTTTGCAATAGGATATTACTTAAAAGAAAAGAAGTGT TTTCCAGAAGGCACAGACATGGTTGGTATATTAGACTTCTACTTCCAGCTGTGCTCCATT GAAGTGACTTGTGAATCAGCCAGTGTGATGGCTGCGACACTGGCTAATGGTGGTTTCTGC CCAATTACTGGTGAAAGAGTACTGAGCCCTGAAGCAGTTCGAAATACATTGAGTTTGATG CATTCCTGTGGCATGTATGACTTCTCAGGGCAGTTTGCTTTCCATGTTGGTCTTCCTGCA AAATCTGGAGTTGCTGGGGGCATTCTTTTAGTTGTCCCCAATGTTATGGGTATGATGTGC TGGTCTCCTCCTCTGGATAAGATGGGCAACAGTGTTAAGGGAATTCACTTTTGTCACGAT CTTGTTTCTCTGTGTAATTTCCATAACTATGATAATTTGAGACACTTTGCAAAAAAACTT GATCCTCGAAGAGAAGGTGGTGATCAAAGGGTAAAGTCAGTGATAAATCTTTTGTTTGCT GCATATACTGGAGATGTGTCTGCACTTCGAAGATTTGCTTTGTCAGCTATGGACATGGAA CAGCGGGACTATGATTCTAGAACAGCACTCCATGTAGCTGCTGCAGAGGGTCATGTTGAA GTTGTTAAATTTTTGCTGGAAGCCTGCAAAGTAAACCCTTTCCCCAAGGACAGGTGGAAT AACACTCCCATGGATGAAGCACTGCACTTTGGACACCATGATGTATTTAAAATTCTCCAA GAATACCAAGTCCAGTACACACCTCAAGGAGATTCTGACAACGGGAAGGAAAATCAAACC GTCCATAAGAATCTTGATGGATTGTTGTAA Clone variation with respect to NM_014905.3 126 a=>n;127 c=>n;128 g=>n;129 g=>n;130 c=>n;131 c=>n;132 g=>n;133 g=>n;134 c=>n;135 c=>n;217 t=>n >OriGene 5' read for NM_014905 unedited
GCACCAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGCTTAGGC GGAGCGAAGAGAACCGGTCGCGGCAATCCTAGCGCGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCAGCACCCGCATCCGCTGCGGGAGTCCGAGCCGGAACCACACCCAAGT AGCTGCCCTTTCCTCTTCTGTCATCTCACCGCCCCACCACAGACCGCGTTCCCCGAGGAA ACCGGCCGCCCACGCCCGGAGCATCCTCCCCTGTTGAGCGGGCGCTGACGGACCCGGCGG CATGATGCGGCTGCGAGGCTCGGGGATGCTGCGGGACCTGCTCCTGCGGTCGCCCGCCGG CGTGAGCGCGACTCTGCGGCGGGCACAGCCCTTGGTCACCCTGTGCCGGCGTCCCCGAGG CGGGGGACGGCCGGCCGCGGGCCCGGCTGCCGCCGCGCGACTCCACCCGTGGTGGGGCGG GGGCGGCTGGCCGGCGGAGCCCCTCGCGCGGGGCCTGTCCAGCTCTCCTTCGGAGATCTT GCAGGAGCTGGGCAAGGGGAGCACGCATCCGCAGCCCGGGGTGTCGCCACCCGCTGCCCC GGCGGCGCCCGGCCCCAAGGACGGCCCCGGNGAGACGGACGCGTTTGGCAACAGCGAGGG CAAAGAGCTGGTGGCCTCAGGTGAAAATAAAATAAAACAGGGTCTGTTACCTAGCTTGGA AGATTTGCTGTTCTATACAATTGCTGAAGGACAAGAGAAAATACCTGTTCATAAANTTAT TACAGCACTCAAATCTACAGGAATGCGAACGTCTGATCCCAGTTGAAAGAGTGTATGGAT ATGNTAAGATTAACTCTTCANACACATCAGATGGTGTCATGCTAGACAAAGACTTTNTAA AAATGGTGTCAGAGCACATNNGTTTGTGACACAGCAT >OriGene 3' read for NM_014905 unedited
CCCTTTATCTCTGCCCCGGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTTT GGAACATATATTTATTATGGCTGGTAAAAAGCAACACTACCTGATTGCATTTAAAATAAA TGTTTCCCAATTTCAGAATACTTACAACTTGTAGTTTTAAGATTAGATTCACTTTGGGAG GTTTTAGAAGCAAATACATTCATAGCTGTGTAATCCCCAGGAAGAATCTAAATCTGACAT CAGGTCATTCAGTCCCTGCCAGACAGACAACAGCATCAAATGGTCAACAGCTAATCCAGC TCTGCAGCTAAAGGGCAGTGTCGGGCAGCAGTGGGGTATAGCATATTACCAAAGATGAGA CCAGCAAAAACAACAATGTGTACTAAGCTTTAAATTAACATGATCATATAGACGGCTCAG GATACAGAAAAAGCACATATGATATTAAAGCCAAAACAAAAAGATCTTTGGAGTTTACTG ACGGCAGCAGTTAATAGCACAGTCAACAGCATTTAAATCCTATATATTATTACCAGCCAA CAGCAAACAGCCCGAGCAGGAATCGGCACATAGTCATAAATAACATCAGGGGTAAATAAC GGCACATTTAATTAAACAACAAAGATTTTACAGAATGGTTTTGATCTGAAAAAAGATGAA AACTCTCTTTGTTCCCAAATAATGGTTAGAACCTCNTTTTATTATAGAAAAGCCTCAAAA TCCTTGGTAATCTTCAAGCCACACAATTCAACTGTCTTGCTTCATTTTAGTCACACAAAG CCGGCCTGCTCTTTGGATAAAGGTATTCTGATTGATTCCAGCTAAAACGTGCCTATCCTG TCATATCCTTCGGGGAAATGCCGCTTCCTGTGCAAATATTTACAAAGCCTACTTGCTTCC ATGCTTTGGTACATCCACGGGGACGGAAACTGTTACTTCACATCCTTCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014905 |
| Insert Size | 5700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014905.2, NP_055720.2 |
| RefSeq Size | 4799 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 2744 |
| UniProt ID | O94925 |
| Domains | ANK, Glutaminase |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Systemic elevation of PTEN induces a tumor suppressive metabolic state
,null,
Cell
,PubMed ID 22401813
[GLS]
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