ARHGAP25 (NM_014882) Human Untagged Clone

CAT#: SC114733

ARHGAP25 (untagged)-Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2



  "NM_014882" in other vectors (6)

CNY 4,760.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ARHGAP25 mouse monoclonal antibody, clone OTI12G4 (formerly 12G4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-308; KAIA0053
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_014882 edited
ATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATG
ACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCC
ATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTAC
TTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAG
GGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCT
GGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCATGGGACAGGAC
TCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGG
AGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCC
TATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTC
ATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTG
GTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACA
GATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTG
GTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAG
GCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGT
CTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAG
ATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAA
GACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATC
AGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAG
AAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAA
GACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACC
AGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGG
GAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGG
AAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAA
AATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATG
TCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCT
TCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCC
AAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGA
GAAGAGGAAATTGATTCTTTGCAGAGGACGGTCCAAGAGCTACGAAAGGAAATAGAAACA
CAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGG
GCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTA
GAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCC
TTGGAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCTTAA
>OriGene 5' read for NM_014882 unedited
GTTCCGATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCCAACCTTT
CGGTTTGCAGAATGACGGGGCCCGCCGCGGTGCCGGACTGCCTGTGCACGGGGCCACCGA
CTGCAGCCTGGGTTTTATTCTTGGCCTGGCCCTGACCGGGAGCTGGCCCCTCGGCTGCTT
CTCTGGCTCGGGGGGGACTTTCTCTGGCTCAGATCCGGACCCCTGAACTGGACCTGGTTG
TCGTCCCCCCGCTTCTCAGCCCCCTCTGGGGTTCCTCTGTCCTCTCCGCCCACTCTTTGC
TCACTGCCCCATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAG
GAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCT
GGAGAGGCCCATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCA
GCAGAGGTACTTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACAC
GAAGCCCCAGGGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCC
AGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCAT
GGGACAGGACTCCTATGTCCTCATGGCCAGCTCTCNAGCGGAGATGGAGGAGTGGGTTAA
ATTCCTCAGGAGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATGA
GACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATG
TGCAGAGTTCATCCTGNAGCACGGCCGGATGAAAAGGCATCTTTCGTCTGCCTGGGCAGN
ACAACCTGNTGAAGCAGCTGAC
>OriGene 3' read for NM_014882 unedited
GTACCGCGGCACGACTTCTATGTCGAGTTTTTTTTTTTTTTTTTTGCTTTATTTGAATAT
AATTTTCTCATCAATTTTGCTTGCATAAATACAGTTTGTTGCTTGTTCTGGTTTAGTCTA
ATAGTAAGTTGGAGAAGTCCTGGAGAATGGGCATCCTGGTGGGGTGGCAGAATGGGGTTC
TCGCTTCAGCAGATGCAGCCCCCCAAACACAGCACTCCATGGATGAGGACTCAAAAGATG
AATGTCATTTTCTGCCCCTGTGTCTCATAGATGCCCTGAGGCTGTCTGACAAGGTGAGCT
GTTCCACACAAAAAACAAAGAATTAATCACACCTTATTCCAGCCTGAGTTGGGGCCAGTC
TATTGCCTGGGTCCTGAGATGTGCTGCCTCAAATGTCTTCACTGGACGTGGTCATGCCTG
AGACCAGAATGGAATAGAACAATCCATTTGAATACAGCGATGGATGGTCAGAGATGTCCA
CACACATCTCTGCTCACCCTCACCTTCCTGCAAACTCCATTCATTTATTTATGGGGAAAT
TTGATGTTTCCCTCAAATGTCCTTTCCCTCTCAGAGAATAATTTTGTTTCCCCGTCATCA
GATAGCACTGAGAAAGGCGCCAGAGTTGGGCCTCTCTGGAGCTGTCCCTGCAGTACTCCT
GGGACCCTTAAGCCTCGGTCTTGGGTTCCTTCATGAATTTGACAAATTCCTTGACTTTCT
CTTTCAAGGCCTTAGTCCTCTTCTCAACATCCTNCCGGGAGCGCTCATGTTGCAGGAGCT
GATCTCTAGGGCTGGAGACTTCTTNCTTTCCTTCTTCAGTTTTATTGAGCCTCACACCTT
AGCCCAAACGTATTATTTTCCTTTCAAGGTTTTAAATCGGTCCCTAAACATTTGCCTCCG
AGTTTCTATTCCTTCCGAAGCTCTTGGACCGNCCTTGCCAAGAACCAATTCCCCTNTCCA
CAATTTTTTTTTCAGCCCCGTTTTAAAGTTGGACTGCCAAAGTACTCCTTGAAGTC
Restriction Sites NotI-NotI     
ACCN NM_014882
Insert Size 2890 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014882.2, NP_055697.1
RefSeq Size 2957 bp
RefSeq ORF 1917 bp
Locus ID 9938
UniProt ID P42331
Domains RhoGAP, PH
Gene Summary ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus, compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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