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E3 SUMO protein ligase PIAS4 (PIAS4) (NM_015897) Human Untagged Clone
CAT＃: SC114580
PIAS4 (untagged)-Human protein inhibitor of activated STAT, 4 (PIAS4)
CNY 3,808.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PIAS-gamma; Piasg; PIASY; ZMIZ6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015897, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGCTCC TGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCA GTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGCCAAGAAGAAC TCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCG GCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAA CGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAAT ATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGT GCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGCCCGGAGTTAA AGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCC AACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGG TGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCG CATCACTGTCACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACC TCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAGGCACTGGTCA AGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCC GCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCC GTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCT ACGACCAGCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGA GTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCC ATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCC TGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGA CCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGAC GAAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGA >OriGene 5' read for NM_015897 unedited
GGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGCCAAGAGGCGG CGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGC TCCTGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCC TCCAGCTGGTGCAGTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACG AGACCCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACC CCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCCAGGACTCCGCTGGCAG GCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAACGGACTGGGACGGTTGC CCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGG ATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCC CGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGC AGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCC CTCAGGAGGACCAGTACCCGCCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTNCG TCCCGGGCTACTACCCCTCCAATAAGCCCGGGGGTGGAGCCCAAAGAGCCGTGCCGNCCC ATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCACCGCATCACTGTCACCTGGG GAACTACNGCAGAGCTACTNCGTGGCCCTGTACCCTGTGCGCAGCTGACCTCATCGNAGC TGCTGCANAGCTGAAGACCATTGGGTAAAGCACCCGNACTGTGCAGGCACTGGTCAAGAG AGCTGCCCTTGATCTGACACGAGTCGCCN >OriGene 3' read for NM_015897 unedited
AAAACCTTTTGCTTTTTTTTTCTATTCCCCCGCTTGGAAGTAGTCCAGTAGGTACAGGAT GCCAGGNAGCCAGTGCAAAAGGGGTGGGAAAAACAAAACGAACGACAATAAAAAGAAAAA GAAGGTCACTCCTCCCTCTGCGCCCGAGGCTGGCCCGTGCCCCTCTGCGTGGTGAGCACC AGGAAAGTCGAGTGTGCGGCCGGGGTCAGCAGGCCGGCACCAGGCCCTTCTGGAAGGGGC AGCGGCGCTTGGGCCGGGGCCCCTCTTCGTCCTCGTCTTCCTCCTCCTCTTCCTCCTCCT CCTCATCCTCCGAGGACGATGAGCTGTCCAGCGTGAGGTCCACCACATCGGCGCCCGGCT TCCCATTCTCCATGCTGCCCACCGGGCCGCCGCCACCCGTGCTGCCCAGGGCACCGCTCC CGTTGACGCTGGGGGCGGGCAGGAGCCCATTGGCGTCCGAGGGGCCCAGCACGAGGATGG CGCCCTGCGGGCTGCAGCTGCGCTCCTTTTCGGCGCGGATCGGGCACCACGAGCCGTCCA CCAGGTACTCGATCTCGTCGGCGTCCTCACACTCGCTCATGATCTTCGAGAGGAGCCCGT CGATGATGAGCTGGTCGTAGGGGGCTGGCTTGTCGCACACGGGGCACATCCAGGTGGGCT TCTTCTCGTTCATCTGCAGGTAGAAGACGGCGTCGAAGCACTGCAGGTGGGCGCAGGTCT CTGCCCGGCAGGGCACGGAGAGCCGCATCTTCACCAGCGGACAGATGATGGACACCCGCA CACCGGTGGTGGCGATCTCGCTGTCAGGATCAAGGCGCAGCTTCTCCTTGACCAGTGCCT TGCACAGCTCCGGGTGCTTTTACCCCATGGTCTTCAGCCTCTGCAGCAGCTCCGATGATG TCAGCTGCCGCACCAGGTACAGGGGCACCGAGTAGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015897 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015897.2, NP_056981.2 |
| RefSeq Size | 1835 bp |
| RefSeq ORF | 1533 bp |
| Locus ID | 51588 |
| UniProt ID | Q8N2W9 |
| Domains | SAP, zf-MIZ |
| Protein Families | Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Caveolin-3 Undergoes SUMOylation by the SUMO E3 Ligase PIASy: SUMOYLATION AFFECTS G-PROTEIN-COUPLED RECEPTOR DESENSITIZATION
,Stephen R. Fuhs and Paul A. Insel,
J. Biol. Chem., Apr 2011; 286: 14830 - 14841
[PIAS4]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206748 | PIAS4 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 3,800.00 |
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| RC206748L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 4 (PIAS4), Myc-DDK-tagged |
CNY 5,890.00 |
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| RC206748L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 4 (PIAS4), mGFP tagged |
CNY 5,890.00 |
|
| RG206748 | PIAS4 (tGFP-tagged) - Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 5,400.00 |
|
| SC324286 | PIAS4 (untagged)-Human protein inhibitor of activated STAT, 4 (PIAS4) |
CNY 3,808.00 |
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