CHST15 (NM_015892) Human Untagged Clone
CAT#: SC114577
CHST15 (untagged)-Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1
CNY 4,184.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BRAG; GALNAC4S-6ST |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC114577 sequence for NM_015892 edited (data generated by NextGen Sequencing)
ATGAGGCACTGCATTAATTGCTGCATACAGCTGTTACCCGACGGCGCACACAAGCAGCAG GTCAACTGCCAAGGGGGCCCCCATCACGGTCACCAGGCGTGCCCCACGTGCAAAGGAGAA AACAAAATTCTGTTTCGTGTGGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTG AGGACTGAAGGGAACGAAAACTGGGGTGGGTTTTTGCGCTTCAAAAAGGGGAAGCGATGT AGCCTCGTTTTTGGACTGATAATAATGACCTTGGTAATGGCTTCTTACATCCTTTCTGGG GCCCACCAAGAGCTTCTGATCTCATCACCTTTCCATTACGGAGGCTTCCCCAGCAACCCC AGCTTGATGGACAGCGAAAACCCAAGTGACACAAAGGAGCATCACCACCAATCCTCTGTA AATAATATTTCATACATGAAGGACTATCCAAGCATTAAATTAATTATCAACAGCATCACA ACTAGGATTGAGTTCACGACCAGACAGCTCCCAGACTTAGAAGACCTTAAGAAGCAGGAG TTGCATATGTTTTCAGTCATCCCCAACAAATTCCTTCCAAACAGTAAGAGCCCCTGTTGG TACGAGGAGTTCTCGGGGCAGAACACCACCGACCCCTACCTCACCAACTCCTACGTGCTC TACTCCAAGCGCTTCCGCTCCACCTTCGACGCCCTGCGCAAGGCCTTCTGGGGCCACCTG GCGCACGCGCACGGGAAGCACTTCCGCCTGCGCTGCCTGCCGCACTTCTACATCATAGGG CAGCCCAAGTGCGGGACCACAGACCTCTATGACCGCCTGCGGCTGCACCCTGAGGTCAAG TTCTCCGCCATCAAGGAGCCACACTGGTGGACCCGGAAGCGCTTTGGAATCGTCCGCCTA AGAGATGGGCTGCGAGACCGCTATCCCGTGGAAGATTATCTGGACCTCTTTGACCTGGCC GCACACCAGATCCATCAAGGACTGCAGGCCAGCTCTGCAAAGGAGCAGAGCAAGATGAAT ACAATCATTATCGGGGAGGCCAGTGCCTCCACGATGTGGGATAATAATGCCTGGACGTTC TTCTACGACAACAGCACGGATGGCGAGCCACCGTTTCTGACGCAGGACTTCATCCACGCC TTTCAGCCAAATGCCAGACTGATTGTCATGCTCAGGGACCCTGTGGAGAGGTTGTACTCA GACTATCTCTACTTTGCAAGTTCGAATAAATCCGCGGACGACTTCCATGAGAAAGTGACA GAAGCACTGCAGCTGTTTGAAAATTGCATGCTTGATTATTCACTGCGCGCCTGCGTCTAC AACAACACCCTCAACAACGCCATGCCTGTGAGGCTCCAGGTTGGGCTCTATGCTGTGTAC CTTCTGGACTGGCTCAGCGTTTTTGACAAGCAACAGTTTCTCATTCTTCGCCTGGAAGAT CATGCATCCAACGTCAAGTACACCATGCACAAGGTCTTCCAGTTTCTGAACCTAGGGCCC TTAAGTGAGAAGCAGGAGGCTTTGATGACCAAGAGCCCCGCATCCAATGCACGGCGTCCC GAGGACCGGAACCTGGGGCCCATGTGGCCCATCACACAGAAGATTCTGCGGGATTTCTAC AGGCCCTTCAACGCTAGGCTGGCGCAGGTCCTCGCGGATGAGGCGTTTGCGTGGAAGACG ACGTGA Clone variation with respect to NM_015892.3 >OriGene 5' read for NM_015892 unedited
NNNNGGTTTANNATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTC TTGTAGAACATGACTCTGGGACATCACTTCCTTTTGTTTTCTTTCGGAGCTGAACCAAAG AATGTGCACCCTCTTTCTCTAGTGCTGTGGTGTCTGCTTATTTTTGTATTTGTGCTTTCC ATCCATCTTCTGTGATCACAAGGCATTCTTAAGGTTTTCTAGCACGACTTGCGGACATCC AGACTCGTGGGGGGCCCACCCATGGCTCGGTAAGCCAGCAGCCCAGGGCACTGGCACTAC CATGAGGCACTGCATTAATTGCTGCATACAGCTGTTACCCGACGGCGCACACAAGCAGCA GGTCAACTGCCAAGGGGGCCCCCATCACGGTCACCAGGCGTGCCCCACGTGCAAAGGAGA AAACAAAATTCTGTTTCGTGTGGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGT GAGGACTGAAGGGAACGAAAACTGGGGTGGGTTTTTGCGCTTCAAAAAGGGGAAGCGATG TAGCCTCGTTTTTGGACTGATAATAATGACCTTGGTAATGGCTTCTTACATCCTTTCTGG GGCCCACCAAGAGCTTCTGATCTCATCACCTTTCCATTACGGAGGCTTCCCCAGCAACCC CAGCTTGATGGACAGCGAAAACCCAAGTGACACAAAGGAGCATCACCACCAATCCTCTGT AAATAATATTTCATACATGAAGGACTATCCAAGCATTAAATTAATTATCAACAGCATCAC AACTANGATTGANGTCACGACCAGACAGCTCCCAGACTTANAAGACCTTAAGAAGCANGA GTTGCATATGTTTTCAGTCATTCCCCACAAATNCCTTNCAACAGTAGAGCCCCTGTTGTA CCAAGATTCTCGG >OriGene 3' read for NM_015892 unedited
TCCTCTTTATTTGTTAAATTTAATAAAGTACAGCGTAACTGCGATTCACTTAACATACCT TACAGGACTAAGGGAGTACAATTTAACACGGTCACAACTTTTGAGAACAAAAAGGAACTT CAATACCATGTTGTGAGAAATGCTCCAATTTGCTTTGGTTTTGAATGGCAGGCTCGAGAA CCACCGCCCATAACGGAACCTATTCTGTCAGCAAGAGCCGGGCCTCGAATGAGAGGAAGC AGAGGCAGCCGAAGTGCCCTCTGGAGAGAAAGGCCCCTGGAGGGAAAAACCATGTCTGGA TGGCATTGAGTGGCACAGAAGGGATGGGACTGCATATATAAAAAAGATCCGCATAATAAA CCAAATAATATTGGAAATAATACCTTCAGTAATACTTCTGTAAGAAGCAGAATTACACCA CATGTTATTCACATGCATAGGAGTGCATAAGAAAAATCCCTTGTTGTAATTATGGTTGGT GTGGAATAGTAAAATATGTACTGAAAATGACAAGTTAATTGGCAGAGAAATTAATCTATA AAAGGGTTGTGTCCAGAGCGGAGGAGCCTCATTAGCAACTGAACAGAACCCACTCAGAAG ACGCACTCACCCACACGTGCGCGTACACACACACACACGCGCGCACTGTACTGCAAATAA ATATACACACACACTGAAGTGATCTCTCCCTAAGCATCCTTGTGCTCATGCCAGATTTCC CTGCTGGCAGCTGCTCCCAGTGCCGGCTATAGAGAATGTGGGGACTGTCCCCTGCAGTGG GATGTCATGACACACGCTCCTTCAGTAGCATAAACAGCCCTGGCCATTCTGGCGCTCATA GCCCTCTGNACACCTTCGAACGGGCTGTGTGTTCCTTCTCTGATGGCAGAATGACAACAC AGCAGAAAGACGGCTGAACACTGCAGATCCTNCTACCCCGGAGCCTGTTCTGCTAACACT ACAANTAAAGCTGGGTAAACGGTTGCTTGCCTTT |
Restriction Sites | NotI-NotI |
ACCN | NM_015892 |
Insert Size | 4350 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_015892.2, NP_056976.2 |
RefSeq Size | 4713 bp |
RefSeq ORF | 1686 bp |
Locus ID | 51363 |
UniProt ID | Q7LFX5 |
Domains | Sulfotransfer |
Protein Families | Transmembrane |
Protein Pathways | Chondroitin sulfate biosynthesis |
Gene Summary | Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 3 encode the same protein. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206489 | CHST15 (Myc-DDK-tagged)-Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1 |
CNY 4,176.00 |
|
RC206489L1 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1, Myc-DDK-tagged |
CNY 6,576.00 |
|
RC206489L2 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC206489L3 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206489L4 | Lenti ORF clone of Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG206489 | CHST15 (tGFP-tagged) - Human carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (CHST15), transcript variant 1 |
CNY 5,776.00 |