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MRPL4 (NM_015956) Human Untagged Clone
CAT＃: SC114544
MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1
CN¥ 2,400.00
CN¥ 2,950.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-28; L4mt |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114544 sequence for NM_015956 edited (data generated by NextGen Sequencing)
ATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGC CTGAGTTCCCTGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGC GAGGGTCTCCCGGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGC CCAGTGCAGGCCTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCC GACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCT ATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTG CGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGC ATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATGGCCCCCGGGGCCCCACAAGT TACTACTACATGCTGCCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTC AAGCTGGCCCAGGACGACCTGCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCA CAGTACCTGACAGAGCTGGCGCACTACCGCCGCTGGGGGGACTCCGTACTCCTCGTGGAC TTAACACACGAGGAGATGCCACAGAGCATCGTGGAGGCCACCTCTAGGCTTAAGACCTTC AACTTGATCCCGGCTGTTGGCCTAAATGTGCACAGCATGCTCAAGCACCAGACGCTGGTC CTGACGCTGCCCACCGTCGCCTTCCTGGAGGACAAGCTGCTCTGGCAGGACTCACGTTAC AGACCCCTCTACCCCTTCAGCCTGCCCTACAGCGACTTCCCCCGACCCCTACCCCACGCT ACCCAGGGCCCAGCGGCCACCCCGTACCACTGTTGA Clone variation with respect to NM_015956.2 >OriGene 5' read for NM_015956 unedited
TATTTCCCCCGCCCGNTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGCGAGGCTCCAGTGGCCTTGACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAG TTCGTCCGGGCCGGGGCGCGGGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCC CTGGCGGAAGAGGCAGCGCGTGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTC CCGGAGCCCGTGCTGCGCAAAGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAG GCCTGGGTCGAGTCCTTGCGGGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCAC CCCGATGTTTTCGCCACCGCGCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAG AAGAACTTCAAGAGAATTAGCTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGGGCGGT GGCCGGAAGCCTTGGCCGCAGAAAGGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCT CCGCTCTGGCGAGGAGGANGTGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTAC ATGCTGNCCATGAAGGTGCGGGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCC CCAGACGACCTGCACATCATGGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTG ACAGAGCTTGCGCACTACCGCCGCTGTGGGGACTTCGTACTCCTCGTGGCTTTATCCACC GAGAGATGCCCAGAGCATCGTGGAGGCCCCTCTATGCTTAAGACCTTCACTTGATCCCGG CTGTTGCCCTAATGTGCCCAGATGCTTCAGC >OriGene 3' read for NM_015956 unedited
NCGGTTTAGCTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTA TCCTCCTTGATTCAAAATACAATTTATTTTAAAAACAAAAAGAGGGAATGGATGCAGAAG CCCGTCTGGGCTCTCCGTGGAGTCGCCCCTTCCCAATGGCGATGGCCGCTCCCTGTGGCC TCAGCTTCCTCCGTGGAATGAAGGGGTCCAGGTGACACCTTCCTCGAAGGGTGGGCGTGG GCCTAAGGCTCCCAAGTCGGCCAGGGGCTCGGCCTGGCACAGAAGAGGTGCTTCACATCA ACAGTGGTACGGGGTGGCCGCTGGGCCCTGGGTAGCGTGGGGTAGGGGTCGGGGGAAGTC GCTGTAGGGCAGGCTGAAGGGGTAGAGGGGTCTGTAACGTGAGTCCTGCCAGAGCAGCTT GTCCTCCAGGAAGGCGACGGTGGGCAGCGTCAGGACCAGCGTCTGGTGCTTGAGCATGCT GTGCACATTTAGGCCAACAGCCGGGATCAAGTTGAAGGTCTTAAGCCTAGAGGTGGCCTC CACGATGCTCTGTGGCATCTCCTCGTGTGTTAAGTCCACGAGGAGTACGGAGTCCCCCCA GCGGCGGTAGTGCGCCAGCTCTGTCAGGTACTGTGGGTCTCCGGTGGGCAGCTCTAGGGA GTCCATGATGTGCAAGTCGTCCTGGGCCAGCTTGACCGTCAGTGCCCCTTTGAGACCCAG CGCCCGCACCTTCATGGGCAGCATGTAGTAATAACTTGTGGGGGCCCCGGGGCCATGGGG CACACCCTCTTCTTCCCAAACCGGAAAGCGGATGCTGCCATGCCCGGGCCCCCCCATGGC CTTTTTGCGGGCAAAGGTTTCGGGCCCCGCCCCCCCCTTGGTTCTTGTTTTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015956 |
| Insert Size | 1250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015956.2, NP_057040.2 |
| RefSeq Size | 1511 bp |
| RefSeq ORF | 936 bp |
| Locus ID | 51073 |
| UniProt ID | Q9BYD3 |
| Domains | Ribosomal_L4 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the predominant transcript and it encodes the same isoform (a) as variant 2. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220629 | MRPL4 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CN¥ 2,400.00 |
|
| RC220629L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC220629L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CN¥ 5,890.00 |
|
| RG220629 | MRPL4 (tGFP-tagged) - Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CN¥ 4,000.00 |
