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PIAS1 (NM_016166) Human Untagged Clone
CAT＃: SC114401
PIAS1 (untagged)-Human protein inhibitor of activated STAT, 1 (PIAS1)
CNY 4,856.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DDXBP1; GBP; GU/RH-II; ZMIZ3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114401 sequence for NM_016166 edited (data generated by NextGen Sequencing)
ATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAA GTACTGTTGGGCTACGCCGGGAGAAACAAGCACGGACGCAAACACGAACTTCTCACAAAA GCCCTGCATTTGCTAAAGGCTGGCTGTAGTCCTGCTGTGCAAATGAAAATTAAGGAACTC TATAGGCGGCGGTTCCCACAGAAAATCATGACGCCTGCAGACTTGTCCATCCCCAACGTA CATTCAAGTCCTATGCCAGCAACTTTGTCTCCATCTACCATTCCACAACTCACTTACGAT GGTCACCCTGCATCATCACCATTACTCCCTGTTTCTCTTCTGGGACCTAAACATGAACTG GAACTCCCACATCTTACATCAGCTCTTCACCCAGTCCATCCGGATATAAAACTTCAAAAA TTACCATTTTATGATTTACTGGATGAACTGATAAAACCCACCAGTCTAGCATCAGACAAC AGTCAGCGCTTTCGAGAAACCTGTTTTGCATTTGCCTTGACACCACAACAAGTGCAGCAA ATCAGTAGTTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTACAGGTCCAGTTA AGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAATCTTTGT GTGAAAGTGAATACAAAACCTTGCAGCCTTCCAGGTTACCTTCCACCTACAAAAAATGGC GTGGAACCAAAGCGACCCAGCCGACCAATTAATATCACCTCACTTGTCCGACTGTCCACA ACAGTACCAAACACGATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAAACTATTCCATG GCAGTATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGAGCAAAG GGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAAGTTGACTGCGGATCCA GACAGTGAAATAGCTACAACCAGCCTAAGGGTTTCTCTACTATGTCCACTTGGTAAAATG CGGCTGACAATTCCGTGTCGGGCCCTTACATGTTCTCATCTACAATGTTTTGACGCAACT CTTTACATTCAGATGAATGAGAAAAAACCAACCTGGGTTTGTCCTGTCTGTGATAAGAAG GCTCCATATGAACACCTTATTATTGATGGCTTGTTTATGGAAATCCTAAAGTACTGTACA GACTGTGATGAAATACAATTTAAGGAGGATGGCACTTGGGCACCGATGAGATCAAAAAAG GAAGTACAGGAAGTTTCTGCCTCTTACAATGGAGTCGATGGATGCTTGAGCTCCACATTG GAGCATCAGGTAGCGTCTCACCACCAGTCCTCAAATAAAAACAAGAAAGTAGAAGTGATT GACCTAACCATAGACAGTTCATCTGATGAAGAGGAAGAAGAGCCATCTGCCAAGAGGACC TGTCCTTCCCTATCTCCCACATCACCACTAAATAATAAAGGCATTTTAAGTCTTCCACAT CAAGCATCTCCAGTATCCCGCACCCCAAGCCTTCCTGCTGTAGACACAAGCTACATTAAT ACCTCCCTCATCCAAGACTATAGGCATCCTTTCCACATGACACCCATGCCTTACGACTTA CAAGGATTAGATTTCTTTCCTTTCTTATCAGGAGACAATCAGCATTACAACACCTCCTTG CTTGCCGCTGCAGCAGCAGCAGTTTCAGATGATCAAGACCTCCTACACTCGTCTCGGTTT TTCCCGTATACCTCCTCACAGATGTTTCTTGATCAGTTAAGTGCAGGAGGCAGTACTTCT CTGCCAACCACCAATGGAAGCAGTAGTGGCAGTAACAGCAGCCTGGTTTCTTCCAACAGC CTAAGGGAAAGCCATAGCCACACCGTCACAAACAGGAGCAGCACGGACACGGCATCCATC TTTGGCATCATACCAGACATTATTTCATTGGACTGA Clone variation with respect to NM_016166.1 318 g=>a >OriGene 5' read for NM_016166 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAAGATGGCGGACAGTG CGGAACTAAAGCAAATGGTTATGAGCCTTAGAGTTTCTGAACTCCAAGTACTGTTGGGCT ACGCCGGGAGAAACAAGCACGGACGCAAACACGAACTTCTCACAAAAGCCCTGCATTTGC TAAAGGCTGGCTGTAGTCCTGCTGTGCAAATGAAAATTAAGGAACTCTATAGGCGGCGGT TCCCACAGAAAATCATGACGCCTGCAGACTTGTCCATCCCCAACGTACATTCAAGTCCTA TGCCAGCAACTTTGTCTCCATCTACCATTCCACAACTCACTTACGATGGTCACCCTGCAT CATCACCATTACTCCCTGTTTCTCTTCTGGGACCTAAACATGAACTGGAACTCCCACATC TTACATCAGCTCTTCACCCAGTCCATCCGGATATAAAACTTCAAAAATTACCATTTTATG ATTTACTGGATGAACTGATAAAACCCACCAGTCTAGCATCAGACAACAGTCAGCGCTTTC GAGAAACCTGTTTTGCATTTGCCTTGACACCACAACAAGTGCAGCAAATCAGTAGTTCCA TGGATATTTCTGGGACCAAATGTGACTTCACAGTACAGGTCCAGTTAAGGGTTTTGTTTA TCAGAAACCAGTTGTCCACAAGAAGATCACTTCCCACCCAATCTTTGTGTGAAAGTGAAT ACAAAACCTTGCAGCCTTTNCAGGTTACCTTCCACCTACAAAAAAATGGCGTGGAACCAA AGCGACCCAGCCGACCAATTAATATCACCTCACTTGTNCGACTGTCCACAACAGTACCCA CACGAATTGTTGTTNCNTGGACTGCAGAAATGGAAGAAACTATTCATGGCAGTATATCTT GTAAAACAGNTGTCCTCAACAGTTCTCTTTCAGAGGTACGGCAAAG >OriGene 3' read for NM_016166 unedited
CGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCTTTCCTTTTTTTTTTTAAA CAAGTGTTTTATCACACAGGCAGTCTTAAATTTATATACAAAAGTAAAACTGAAAATATA GTTTTGTTCTCTGTACATTTCTTTTAATTTTTTCCCTAAAAAAAGGAAAAAAAACACGCT TGTATACTTTTCTAAACAGAGTAAGGTAAAACAGAGCACAAAGTTCTCTTCTTTCTGCCA AGTTCATTCGATCTGGGGTGGGGATGGGAGCAGCAGGGCCTGGGAATCAGTCCAATGAAA TAATGTCTGGTATGATGCCAAAGATGGATGCCGTGTCCGTGCTGCTCCTGTTTGTGACGG TGTGGCTATGGCTTTCCCTTAGGCTGTTGGAAGAAACCAGGCTGCTGTTACTGCCACTAC TGCTTCCATTGGTGGTTGGCAGAGAAGTACTGCCTCCTGCACTTAACTGATCAAGAAACA TCTGTGAGGAGGTATACGGGAAAAACCGAGACGAGTGTAGGAGGTCTTGATCATCTGAAA CTGCTGCTGCTGCAGCGGCAAGCAAGGAGGTGTTGTAATGCTGATTGTCTCCTGATAAGA AAGGAAAGAAATCTAATCCTTGTAAGTCGTAAGGCATGGGTGTCATGTGGAAAGGATGCC TATAGTCTTGGATGAGGGAGGTATTAATGTACCTTGTGTCTACAGCAGGAAGGCTTGGGG TGCGGNATACTGGAGATGCTTGATGTGGAAGACTTANAATGCCTTATTATTTANTGGTGA TGTGGGAGATAGGAAAGACAGGTCCCTCTGGCAAATAGCTCCTCCTTCTCTTCATCANAT GAACTGCCTATGGNTANGTCAATCACTTCTACTTTCCTGNTTTTATTTGAGGACCGGNTG GTGAGCACCCTACCTGATGCTCCATGGGGGAACTACAGCTTCATCGACTCCATTGGTAGA AGCAAAAACTNCCTGGACTCCCTTTTTGAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016166 |
| Insert Size | 2320 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016166.1, NP_057250.1 |
| RefSeq Size | 2309 bp |
| RefSeq ORF | 1956 bp |
| Locus ID | 8554 |
| UniProt ID | O75925 |
| Domains | SAP, zf-MIZ |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) uses an alternate 5' structure which results in a distinct 5' UTR and 5' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219594 | PIAS1 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 1 (PIAS1) |
CNY 4,848.00 |
|
| RC219594L1 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), Myc-DDK-tagged |
CNY 7,248.00 |
|
| RC219594L2 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), mGFP tagged |
CNY 5,890.00 |
|
| RC219594L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219594L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 1 (PIAS1), mGFP tagged |
CNY 5,890.00 |
|
| RG219594 | PIAS1 (tGFP-tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1) |
CNY 6,448.00 |
