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NAGPA (NM_016256) Human Untagged Clone
CAT＃: SC114360
NAGPA (untagged)-Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
CNY 3,840.00
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APAA; UCE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114360 sequence for NM_016256 edited (data generated by NextGen Sequencing)
ATGGCGACCTCCACGGGTCGCTGGCTTCTCCTCCGGCTTGCACTATTCGGCTTCCTCTGG GAAGCGTCCGGCGGCCTCGACTCGGGGGCCTCCCGCGACGACGACTTGCTACTGCCCTAT CCACGCGCGCGCGCGCGCCTCCCCCGGGACTGCACACGGGTGCGCGCCGGCAACCGCGAG CACGAGAGTTGGCCTCCGCCTCCCGCGACTCCCGGCGCCGGCGGTCTGGCCGTGCGCACC TTCGTGTCGCACTTCAGGGACCGCGCGGTGGCCGGCCACCTGACGCGGGCCGTTGAGCCC CTGCGCACCTTCTCGGTGCTGGAGCCCGGTGGACCCGGCGGCTGCGCGGCGAGACGACGC GCCACCGTGGAGGAGACGGCGCGGGCGGCCGACTGCCGTGTCGCCCAGAACGGCGGCTTC TTCCGCATGAACTCGGGCGAGTGCCTGGGGAACGTGGTGAGCGACGAGCGGCGGGTGAGC AGCTCCGGGGGGCTGCAGAACGCGCAGTTCGGGATCCGCCGCGACGGGACCCTGGTCACC GGGTACCTGTCTGAGGAGGAGGTGCTGGACACTGAGAACCCATTTGTGCAGCTGCTGAGT GGGGTCGTGTGGCTGATTCGTAATGGAAGCATCTACATCAACGAGAGCCAAGCCACAGAG TGTGACGAGACACAGGAGACAGGTTCCTTTAGCAAATTTGTGAATGTGATATCAGCCAGG ACGGCCATTGGCCACGACCGGAAAGGGCAGCTGGTGCTCTTTCATGCAGACGGCCAAACG GAGCAGCGTGGCATCAACCTGTGGGAAATGGCGGAGTTCCTGCTGAAACAGGACGTGGTC AACGCCATCAACCTGGATGGGGGTGGCTCTGCCACCTTTGTGCTCAACGGGACCTTGGCC AGTTACCCGTCAGATCACTGCCAGGACAACATGTGGCGCTGTCCCCGCCAAGTGTCCACC GTGGTGTGTGTGCACGAACCCCGCTGCCAGCCGCCTGACTGCCACGGCCACGGGACCTGC GTGGACGGGTACTGCCAATGCACCGGGCACTTCTGGCGGGGTCCCGGCTGTGATGAGCTG GACTGTGGCCCCTCTAACTGCAGCCAGCACGGACTGTGCACGGAGACCGGCTGCCGCTGT GATGCCGGATGGACCGGGTCCAACTGCAGTGAAGAGTGTCCCCTTGGCTGGCATGGGCCG GGCTGCCAGAGGCCTTGTAAGTGTGAGCACCATTGTCCCTGTGACCCCAAGACTGGCAAC TGCAGCGTCTCCAGAGTAAAGCAGTGTCTCCAGCCACCTGAAGCCACCCTGAGGGCGGGA GAACTCTCCTTTTTCACCAGGACCGCCTGGCTAGCCCTCACCCTGGCGCTGGCCTTCCTC CTGCTGATCAGCATTGCAGCAAACCTGTCCTTGCTCCTGTCCAGAGCAGAGAGGAACCGG CGCCTGCATGGGGACTATGCATACCACCCGCTGCAGGAGATGAACGGGGAGCCTCTGGCC GCAGAGAAGGAGCAGCCAGGGGGCGCCCACAACCCCTTCAAGGACTGA Clone variation with respect to NM_016256.3 1030 c=>t;1394 c=>t >OriGene 5' read for NM_016256 unedited
GTTCACGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGAATAT GGCGACCTCCAGGGTCGCTGGCTTCTCCTCCGGCTTGCACTATTCGGCTTCCTCTGGGAA GCGTCCGGCGGCCTCGACTCGGGGGCCTCCCGCGACGACGACTTGCTACTGCCCTATCCA CGCGCGCGCGCGCGCCTCCCCCGGGACTGCACACGGGTGCGCGCCGGCAACCGCGAGCAC GAGAGTTGGCCTCCGCCTCCCGCGACTCCCGGCGCCGGCGGTCTGGCCGTGCGCACCTTC GTGTCGCACTTCAGGGACCGCGCGGTGGCCGGCCACCTGACGCGGGCCGTTGAGCCCCTG CGCACCTTCTCGGTGCTGGAGCCCGGTGGACCCGGCGGCTGCGCGGCGAGACGACGCGCC ACCGTGGAGGAGACGGCGCGGGCGGCCGACTGCCGTGTCGCCCAGAACGGCGGCTTCTTC CGCATGAACTCGGGCGAGTGCCTGGGGAACGTGGTGAGCGACGAGCGGCGGGTGAGCAGC TCCGGGGGGCTGCAGAACGCGCAGTTCGGGATCCGCCGCGACGGGACCCTGGTCACCGGG TACCTGTCTGAGGAGGAGGTGCTGGACACTGAGAACCCATNTGTGCAGCTGCTGAGTGGG GTCGTGTGGCTGATTCGTAATGGAAGCATCTACATCAACGAGAGCCAAGCCACAGAGTGT GACGAGACACAGGAGACAGGTTCCTTTAGCAAATTTGTGAATGTGATATCAGCCAGGACG GCCATTGGCCACGACCGGNAAGGGCAGCTGGTGCTCTTTCATGCAGACGGGCAAACGGAG CAGCGTGGCATCAACCTGTGGGAAATGGCNNGAGTNCTGCTGAANCAGACGTGGTCACGC ATCACCGTGAGGGGGGGGGCTCTGCCACCTTGTGCTCACG >OriGene 3' read for NM_016256 unedited
GGGGGGCCGANGNNAGNNNNTTTTNNNNNNGNNTTTCNGGNACCGGAGCCGCATTTTATA TNCGGTTTTTTTTTCTTTTTTTTTCCTTTTATTATATCAATTTATTCAAGTGATTAAAAA ACTTCTTGGCAGCAGGATGACAAAACAGGTCTGTAAACATGATTTCATCCCCCCATCCCT TCTTCCAAGGAGCTTCCTATGACAGGAAATCGAATTCTAGTTGGCAAATCCAGTGATGAG GTCTGTAGAAAAGGGGTCCCGTGTCACAGCCAGGAAGGCAGACTCGTCCCTTTAACCCAC TCCAGCCAGGGGTGCTGGCCAGGATGTGCTGTTCTGTCATGACGTGGTCACTGAGTCTGG TTCGTTGGCAGCCCCTTCCCCAGGAGGAGGGAGACTCTTTGGCAGTGCGGCAGCCCTCCC GGGGGCACGGGCTTCTCGGCAGCAGACACAGGCAGGCGAGAAGCAGGCAGCTGAGCCTCT CCAGCGAGCATGGTATTGCTAGGGTTGCAGGTGCCCTGNCAGGTGGCCAGGTGAGGGGCT GAGGCACAAGTGCTATCAGGAACTTGGCTGCCCCACAGGGGCTGANGACACCCAGATGGT CCACGCCAGTGGCCTTGAAATTTCCCCTGCAGAAGCCAGACCGTGGGGAAACAAGCTNTC GCGACGTGCCACCCCCGGCAGCTTGAGGCTTCAGTCCTTGAGGGGGNTGTGGGCGCCCCC TGGNCTGCTCTTCTCTGCGGCCAGAAGCTCCCCGTNCATCTCCTGCAGCCGGTGGTATGC ATAGNTCCCATGCNAGCGCCGGTTCCTCTCTGCTCTGGACAGAGCAAGACAGGNTTGCTG CATGCTGATCNCAAGAGGAAGCCACCCCCGGTGAGGGCTACCCAGCCGCCCTGTGAAAAA GAAAGTCCTCCGCCTCAAGGGGGCTAAGGTGCTGGAAACATGTTTACTTTGAAACCCTTC ANTGGCAATCTGGGGTCACAGGCATGGGGCCCACACTACAAGCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016256 |
| Insert Size | 2290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016256.2, NP_057340.2 |
| RefSeq Size | 2219 bp |
| RefSeq ORF | 1548 bp |
| Locus ID | 51172 |
| UniProt ID | Q9UK23 |
| Domains | EGF |
| Protein Families | Transmembrane |
| Protein Pathways | Lysosome |
| Gene Summary | Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in this gene are associated with developmental stuttering in human patients. [provided by RefSeq, Oct 2015] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218271 | NAGPA (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) |
CNY 3,832.00 |
|
| RC218271L1 | Lenti ORF clone of Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), Myc-DDK-tagged |
CNY 6,232.00 |
|
| RC218271L2 | Lenti ORF clone of Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mGFP tagged |
CNY 6,370.00 |
|
| RC218271L3 | Lenti ORF clone of Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC218271L4 | Lenti ORF clone of Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA), mGFP tagged |
CNY 6,370.00 |
|
| RG218271 | NAGPA (tGFP-tagged) - Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) |
CNY 4,940.00 |
