POLR3K (NM_016310) Human Untagged Clone
CAT#: SC114310
POLR3K (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | C11; C11-RNP3; HLD21; My010; RPC10; RPC11; RPC12.5 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_016310 edited
ATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAGGAGGGACAACGCTGC CACCGCTTCGCCTGCAACACGTGCCCCTACGTGCACAACATCACCCGCAAGGTAACAAAT CGGAAGTACCCAAAACTGAAAGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAG AATGTTGACTCTACTGCAGAGTCGTGTCCCAAATGCGAACATCCTCGTGCTTACTTCATG CAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCTTCTACAAGTGCTGCAATGCT CAGTGTGGACACCGCTGGAGGGATTAG >OriGene 5' read for NM_016310 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCCGGGAGTTGGAGCCT GCGGAGTTCGAGACCATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAG GAGGGACAACGCTGCCACCGCTTCGCCTGCAACACGTGCCCCTACGTGCACAACATCACC CGCAAGGTAACAAATCGGAAGTACCCAAAACTGAAAGAAGTGGATGATGTGCTTGGTGGA GCAGCTGCCTGGGAGAATGTTGACTCTACTGCAGAGTCGTGTCCCAAATGCGAACATCCT CGTGCTTACTTCATGCAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCTTCTAC AAGTGCTGCAATGCTCAGTGTGGACACCGCTGGAGGGATTAGGGCCAGGATGGCCCAGCT GCCCTAGTGTGTGCTTGCCTTGTCCCTCGGGGTAGATGCTTAGCTGGCAGTATGAGTCGT GTGTCCTGAGGGTCTTTGCTAGTGTGGTGGAAAGATAAACCTTTTGAGGTGAAGAGCCAG GGGGTCAGGAAATATGGCCTATCTGCCAGGCAGGGTGGATGAAGTCATGAATGTCTGGGA GTTTTTCTGTGTGGGGAGGAGACAGAGACCCATAACTAAATATGCTCTGTGTAAAGTCCT ATTCTTTCATCTTCCACTNTATTGGCAGTTGACATTCCCTTACTCCCAATCAACACTCTT AAATATTTGTACTGTTTGTAAAACTTAGTACATGTCCCCTAATATTTANNANNANNANNN NNNNNNANANNAAAACTCGACTCTAGATTGCGCCGCGTCATAGCTGTTCCTGACAGATCC GGNTGCATCCTGTGACCCTNCCAGTGCTCTCTGGCCTGAAGTGCACTCAGTGCACCANCT GNCTATAAAATAGTGCATATTGCTGACTAGGCTATATTTGAGGGGGGGGGTG >OriGene 3' read for NM_016310 unedited
GTCCGAGGCCGCTTTCTANATCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA TATTTAGGGACATGTACTAAGTTTTACAAACAGTACAAATATTTAAAAGTGTTGATTGGG AGTAAGGGAATGTCAACTGCCAATAAAGGGGAAGATGAAAGAATAGGACTTTACACAGAG CATATTTAGTTATGGGTCTCTGTCTCCTCCCCACACAGAAAAACTCCCAGACATTCATGA CTTCATCCACCCTGCCTGGCAAATAGGCCATATTTCCTGACCCCCTGGCTCTTCACCTCA AAAGGTTTATCTTTCCACCACACTAGCAAAGACCCTCAGGACACACGACTCATACTGCCA GCTAAGCATCTACCCCGAGGGACAAGGCAAGCACACACTAGGGCAGCTGGGCCATCCTGG CCCTAATCCCTCCAGCGGTGTCCACACTGAGCATTGCAGCACTTGTAAAAGGTGGTCATC GGCTCATCTGCAGAGCGGGTCTGAAGCTGCATGAAGTAAGCACGAGGATGTTCGCATTTG GGACACGACTCTGCAGTAGAGTCAACATTCTCCCAGGCAGCTGCTCCACCAAGCACATCA TCCACTTTTTTCAGTTTTGGGTACTTCCGATTTGTTACCTTGCGGGTGATGTTGTGCACG TAGGGGCACGTGTTGCAGGCGAAGCGGTGGCAGCGTTGTCCCTCCTCCACGATCAGCCCG TTCCCGCAGCCGGGGCAGAACAGCAGCATGGTCTCGAACTCCGCAGGCTCCAACTCCCGG CAGCCTTGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGT TCACTAAACGAGCTCTGCTTTATAGACCTCCACCGTACACGCCTACCGCCATTTGGGTCA ACGGGCGGGGTTATACGACATTTGGGAAGTCCGT |
Restriction Sites | NotI-NotI |
ACCN | NM_016310 |
Insert Size | 820 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016310.2, NP_057394.1 |
RefSeq Size | 796 bp |
RefSeq ORF | 327 bp |
Locus ID | 51728 |
UniProt ID | Q9Y2Y1 |
Domains | TFIIS, RNA_POL_M_15KD |
Protein Families | Transcription Factors |
Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
Gene Summary | This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204102 | POLR3K (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K) |
CNY 1,200.00 |
|
RC204102L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204102L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), mGFP tagged |
CNY 5,890.00 |
|
RG204102 | POLR3K (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K) |
CNY 2,800.00 |