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DIP13B (APPL2) (NM_018171) Human Untagged Clone
CAT＃: SC113670
APPL2 (untagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2)
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DIP13B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018171, the custom clone sequence may differ by one or more nucleotides
ATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGCGTTGCAGGACAGCCCCCAGACTCGCTCTTTACTGA GCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATACCAACCAGCTGCTCCAGGCAATGCAGCGCGT CTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAA CAGAACTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGG ATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATACA ATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTAGCAATGAGCAT GACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAGGTGAAGACCGAAGTCG GAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCACCTCTCCTCCCTTCAGTACTACTGTGCCCTCAACGC GCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCCCATGGACAGATTAACTTT TTTAAGAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCCTCCGTTGCAGACATGGTTC AAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCAAGAATTACTTTCTGT TGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATCAACAGGAACCTCATCCAGAAG GCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACCTGGGAGAGGCTTTATTTCTTCA CCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGAGGTTTGATCCAGGACCTGGACAA CTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTACTGCTTCCAGATCACCACGCCCAATGGAAAA TCGGGAATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAGTGGATATGTGCAATAAACAACATCT CCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATCAAGTTGAATCAGACCGCTCTGCAAGC AGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATGCCCCAGCCAGAACCTGAAAAATTCA GAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGCAACAGCCAGTCTACCTGAAGCAGAGGAGCTGA TCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCTGCTACAGAATTCCTTGATCAGAACAGAGG GAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAATCATTTCCAGAAGCAGAAGATTCTCTTTTG CAGCAGATGTTTATAGTTCGGTTTTTGGGATCAATGGCAGTTAAAACAGACAGCACTACTGAAGTGATTT ATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATTCATAACATCTTCCGCATGACAGAATCCCATCT GATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCACAGACTCAAGTATCAAGGGCCAATTTTGAACTT ACCAGTGTCACACAATTTGCTGCTCATCAAGAAAACAAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTG AATCCACTGGAGAAGAATCTCTGAGTACATACATTTTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTA TGCTATTAATTTGGGAAAAGAAATTATTGAGGTTCAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTG TCCATACCACTAACCAATGACGGAAAATATGTACTGTTAAACGATCAACCAGATGACGATGATGGAAATC CAAACGAACATAGAGGCGCAGAATCCGAAGCATAA >OriGene 5' read for NM_018171 unedited
GGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTGTAAAACT AGTCTGTGTGCATACACATGCGTTATTTCTGCTTACCTCTCTATGTGGGAAGCTGAACAT TTTAGGGATTAAGAAATCTGCCTCCATTGTTGTTTAGAAGCCTCTGCTCCTTGCCTGGCA CTACTGGCTGTTGGGAATGTGAAGATAAATAAGAGTGACTACCCTTGACTGGTTCACAGT TTGTTCCAGAGACAGATAATGAGGCAACAGTTCCAGTACAGTGTGAATCCTGCTGCGATA GGAACACAAATGCAGCTTTGAGCTCCTGCAGCTTGTACTTCTTCAGGGGCTTATTCTCTT GTCCTACTCGCTCTTTACTGAGCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATA CCAACCAGCTGCTCCAGGCAATGCAGCGCGTCTATGGAGCCCAGAATGAGATGTGCCTGG CCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAACAGAACTTTGCTCTTGGCA AAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGGATGAGCTTA ATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATAC AATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTA GNCATGAGCATGACCTCTCAATGGCANAATACAGCANGCTGCCTAAGAAAAAGGAGATGA GAAGGTGAAGACCCGAAGTCGAAAAGAGTGGGCCGCGCCCNGNCGAAGCAGCACCTCTCC TCCCTTCAGTACTACTGTGCCCTCCACGCGCTGCAGTACAGAAAAGCAATGGCCATGATG GAGCCC >OriGene 3' read for NM_018171 unedited
GCTATGCACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAAAAA AACAAAATAATTTTTATTGCTAGGTTAATTTATTACAAAGATAATAATAGTCTGTTGAAC TTAGTTACAGTTAAACACACTGTACCGATTCAAAATCATTATACTAGTTTGATAGGGAGG AGGGATGGGGGAGCGGGCTGGGGTTCCAGGAATTGGAAGTATCAGGGTGGAAATAGGAAC TTTATACCAAGCCCCATGACCAGCTGTGCTTCAAGTGAGTTTTAAAATTACCTGGTCCCT TCCCTGAAGAGGGTCTGGCTCAAAAGAAACAGGAACTGGCAGAGCTGCACCCTGTCCACA GTGATCCACTACTAAAAATACTCATACCTAGGAGGACCTTAAACTTTTGAATGCTGCTTC AGCAAGGCTCCACTGTAAAATCAAACATTTTTTGCGGTACTTATTTTTTTATTGCTTGAT GCCTGGAACAGTTACCGAAACTAGACCTGCATCGCGCATCTCTATTACTGTTTGGGCCTC AAAGCAGTGCTCGTCACCTAACTGCACAAAGAACCGCTATTACGCCCAATTGTGACATAA GCTGAATTATAAATACACAATTTTCAGAACGACAGGCACTCAACGCTCACTCCTTAACCC CATACACTCCACCTCTTAATAACAAATATTTAGGGCTCATTAAACTTGAAACCTAGACTT TACGCCTTTTTTAAAAAGATACTGGCAACTGTAAAAAATGACCTTAATAGGACAACGCTT CTCCAAATTTTTGTATAGCGCTGAAATTCCACAAATTTACTGCACCCAACAAAAAGATTT CCCCAAATTCCTTAAACACTTCGTAAAGGTCGGTAACCTTGCCATCATTTTCTACTCCCC TTCACACCTCACATTTATGGTTAAAACCCCCTCCCCAACTATGTCCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018171 |
| Insert Size | 3370 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018171.2, NP_060641.2 |
| RefSeq Size | 3289 bp |
| RefSeq ORF | 1995 bp |
| Locus ID | 55198 |
| UniProt ID | Q8NEU8 |
| Domains | PH, PID |
| Gene Summary | The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) encodes the predominant isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207506 | APPL2 (Myc-DDK-tagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2) |
CNY 4,944.00 |
|
| RC207506L1 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), Myc-DDK-tagged |
CNY 7,344.00 |
|
| RC207506L2 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), mGFP tagged |
CNY 6,460.00 |
|
| RC207506L3 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207506L4 | Lenti ORF clone of Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2), mGFP tagged |
CNY 6,460.00 |
|
| RG207506 | APPL2 (tGFP-tagged) - Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2) |
CNY 6,544.00 |
