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NGLY1 (NM_018297) Human Untagged Clone
CAT＃: SC113603
NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1
CNY 7,800.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDDG; CDG1V; PNG-1; PNG1; PNGase |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018297, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACA CCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAA TGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA GCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAAGCTT CAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGCTCAAA TAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCA AATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCTGCTTCAA CGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAA TCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCACAAGAAAAG TTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTGCTGGAGCTTT TGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGTGGTGGACAGAC TAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGAT CATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAACTTTTGGAAA CAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAGC TCGCTATGTTTGGGATTACACAGACCATGTCTGGACAGAAGTCTATTCTCCTTCTCAGCAGCGGTGGCTG CACTGTGATGCATGTGAAGATGTCTGTGACAAGCCACTCCTTTATGAAATAGGATGGGGCAAGAAGCTTT CCTATGTCATAGCATTTTCAAAAGATGAGGTAGTTGATGTCACTTGGCGATATTCCTGCAAACATGAAGA GGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAATGGGCTTAATAAGCAG AGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAAT TTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGTCAGTGGCTTGGAGAGT AGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGAAAATGAGAAGATTTCT AAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCAAATAACAATCAAACCA TTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTGAAACAGACTGGCACAT GGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAGTGTGGGTCAGTT GGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACTGGAACAGTAGAATGGA AATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACTCCTATGCTGATTTTTC TGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCGCTTGGCAACACACC CAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATTATAAAATTCAGTGACC TTTGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018297 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018297.2, NP_060767.2 |
| RefSeq Size | 2182 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 55768 |
| UniProt ID | Q96IV0 |
| Domains | TGc, PUG, PAW |
| Gene Summary | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203447 | NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 7,312.00 |
|
| RC203447L1 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 9,712.00 |
|
| RC203447L2 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 5,890.00 |
|
| RC203447L3 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 5,890.00 |
|
| RC203447L4 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 9,712.00 |
|
| RG203447 | NGLY1 (tGFP-tagged) - Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 8,912.00 |
|
| SC320763 | NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 |
CNY 7,312.00 |
