RFK (NM_018339) Human Untagged Clone
CAT#: SC113557
RFK (untagged)-Human riboflavin kinase (RFK)
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RIFK |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC113557 sequence for NM_018339 edited (data generated by NextGen Sequencing)
ATGAGGCACCTGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCGGCCGCGGCTCC AAGCAGCTGGGCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGCT GATATATCCACTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAG ATGGTGGTGAGCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACA CATATCATGCATACCTTCAAAGAGGACTTCTATGGGGAAATCCTCAATGTGGCCATTGTT GGCTACCTGAGACCAGAAAAGAACTTTGATTCTTTAGAGTCACTTATTTCAGCAATTCAA GGTGATATTGAAGAAGCTAAGAAACGACTAGAGTTACCAGAACATTTGAAAATCAAAGAA GACAATTTCTTCCAGGTTTCTAAAAGCAAAATAATGAATGGCCACTGA Clone variation with respect to NM_018339.5 >OriGene 5' read for NM_018339 unedited
GTCAGGATTTGAATACGACTTCTATAGGCGGCCCGCGATTCGGCACGAGGCGGCGTCAGG TTATTCGCAGTGGCCTGTGGTCGGCGTCGTCCGGCCACTGGTGCGCCCCCGCGGCAGGCA GAGCTCACGCTCCTGTCCCCCGGCTGGTCCGGGGTCTGGGCGCCGCGTCGACGGCGGCCC CGCAGGACGCGCAGACCGGGCCGCAGCCCATGCCCCGAGCGGACTGCATTATGAGGCACC TGCCTTACTTCTGCCGGGGTCAAGTGGTGCGGGGCTTCGGCCGCGGCTCCAAGCAGCTGG GCATCCCCACAGCTAATTTTCCTGAGCAAGTGGTAGATAATCTTCCAGCTGATATATCCA CTGGTATTTACTATGGTTGGGCCAGTGTTGGAAGTGGAGATGTCCATAAGATGGTGGTGA GCATAGGATGGAACCCATATTACAAGAATACGAAGAAGTCTATGGAAACACATATCATGC ATACCTTCAAAGAGGACTTCTATGGGGAAATCCTCAATGTGGCCATTGTTGGCTACCTGA GACCAGAAAAGAACTTTGATTCTTTAGAGTCACTTATTTCAGCAATTCAAGGTGATATTG AAGAAGCTAAGAAACGACTAGAGTTACCAGAACATTTGAAAATCAAAGAAGACAATTTCT TCCAGGTTTCTAAAAGCAAAATAATGAATGGCCACTGATGAAAAATTGTATTATTTATTC ATTCACTGTTTTCTAGGGTTNCTGTGTTTATTACTGGTCAGCATCATCTTGGTTATATAC TGGAAATCAACACTTTACTACAGTTGTGAATTAGTTTAAACCGTACCATGGTGNTATCAT ATCATGCTTCAACTATTAAATTAGCCCATCATATCATAGCACTAAAAAGATGAGTTAAAA ATAAGATTACAAATTTT >OriGene 3' read for NM_018339 unedited
CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC AACTAAGCCAAAATTTATTTAAGGGGCCTGAAAACTTTCAAACTAATCAAAAGGGTCCTA CGGTAACTTTTTATAATACATAATATAAAAGTTTTTGAAAAATATAAACCCATTTAACCC CTAAACAACACATTTTTTGATTTTAAAAAGCAAGGGTTTTTTAACAAGATGTAAAGGGAC ATTACCTTTTCAAAAAACTTTCCCCCCCCTAAAAATAGCTTTTACCAGCAGGTTATTCAA ACAAAACAAACACAAATTTTTCCACATTCCCTATGTTGGTTTTAAACTTTACTTCATAAA GCCACTGATAATGGGGGTTTTTTTCAAGTTTAAAATTTCTAAAATTAAAAACTGTTTTTG ACATATTTTTATAAAAAAATAAAAAGCAAAACGCATTCCAACTTTTTATATGAGCCCCTT TTCTCCAACAGCTTTAAAGGTTTTTCTGAGTCCTTTTTACACAAAATATTTTTATTAAAA TCAGTTCTAATTCTTTTATGCAAATTAGGGGAAAAGGATTCATAATAAATTAACTTTAAA ATTACCTTTTATCTGTTTCTACCTTTATCCCCCCATCACCACCAAATCTGTTGCTACAGG GAACTGTACCAATGTACTGTTTGAGGGGGCCCAAACCATCTGTAATCTTAATTTCCCCAT ATTAGGTAGTAGGAGGAAAAATCCACAGTTGAAGGCTATTTAACTAAAACTTTTGCTTTT CCCTAAGCTGTAGGACTTTTTACATTAAATACATCGGCATTACACTACACCCTTTAAATC GCTTGAATATATTCAGGCCTAAATTAAAAAATTTATACTTTCAAGNTATTGGCTTTAAAG TTTGCAGGAGAACATTGTGTCTAAAATGATGGGAAGGGTCCTAATCTGAAAACTAGAGAC CGGGT |
Restriction Sites | NotI-NotI |
ACCN | NM_018339 |
Insert Size | 3040 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018339.3, NP_060809.2 |
RefSeq Size | 2712 bp |
RefSeq ORF | 489 bp |
Locus ID | 55312 |
UniProt ID | Q969G6 |
Domains | FAD_Synth |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Riboflavin metabolism |
Gene Summary | Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202812 | RFK (Myc-DDK-tagged)-Human riboflavin kinase (RFK) |
CNY 1,200.00 |
|
RC202812L3 | Lenti ORF clone of Human riboflavin kinase (RFK), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202812L4 | Lenti ORF clone of Human riboflavin kinase (RFK), mGFP tagged |
CNY 5,890.00 |
|
RG202812 | RFK (tGFP-tagged) - Human riboflavin kinase (RFK) |
CNY 2,800.00 |