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MBD5 (NM_018328) Human Untagged Clone
CAT＃: SC113547
MBD5 (untagged)-Human methyl-CpG binding domain protein 5 (MBD5)
CNY 10,232.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRD1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113547 sequence for NM_018328 edited (data generated by NextGen Sequencing)
ATGAATGGAGGCAAAGAGTGTGACGGAGGGGACAAGGAAGGAGGTCTTCCAGCTATACAA GTTCCTGTGGGTTGGCAGCGTCGTGTGGATCAAAATGGAGTGCTTTATGTCAGTCCCAGT GGGTCTTTGTTATCTTGCTTGGAGCAGGTTAAAACATACCTGCTTACTGATGGAACATGC AAGTGTGGCTTGGAATGTCCTCTTATTCTTCCCAAGGTATTTAATTTTGATCCTGGAGCT GCTGTGAAACAGAGAACCGCAGAAGATGTTAAGGCAGATGAAGATGTCACAAAGCTATGC ATACATAAAAGAAAAATTATTGCAGTGGCCACACTTCATAAAAGCATGGAAGCCCCACAT CCTTCTCTGGTGCTCACCAGTCCCGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCT CGGGCAGCAACTCCAAGATCAGTAAGAAATAAGTCTCATGAAGGAATTACAAATTCTGTA ATGCCTGAATGTAAGAATCCTTTCAAGTTAATGATTGGATCATCAAATGCCATGGGAAGG CTATATGTACAAGAACTGCCTGGAAGCCAACAACAAGAACTCCACCCTGTCTACCCCCGA CAGAGATTGGGCAGCAGTGAACATGGACAGAAATCTCCATTCCGTGGCAGCCATGGAGGC CTGCCCAGCCCAGCGTCATCAGGTTCCCAGATATATGGAGATGGTTCAATCTCTCCAAGG ACTGACCCACTTGGAAGTCCTGATGTTTTCACAAGAAGTAATCCTGGTTTTCATGGAGCT CCCAATTCTAGTCCTATTCACCTGAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA CATGGTTCTCCTGTACAGTCATCCTGTGCAATGGCTGGAAGGACTAATATACCTCTTTCC CCAACCTTGACTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAAT ATGGAAATACCACGAGCAATGTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCT CCACCTTCTTGTGCTCTTCAGAAAAAGCCATTAACATCTGAGAAAGATCCACTTGGCATT CTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTGTTATCATTAATCCAACCAGT TTCCATTCAAATGTCCACTCTCAGGTACCTATGATGAATGTAAGCATGCCTCCTGCTGTT GTTCCTTTGCCAAGTAATCTCCCATTGCCAACTGTAAAACCTGGTCACATGAATCATGGG AGTCATGTACAAAGAGTTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACA TCCCCCGTGCACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCA CGCTCATCTTCCACATCATCAGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAG GCCACTTCTAGTGGTATTAAGGTTCCACCCAGGTCACCAAGGTCAACAATAGGGTCCCCA AGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCCGATGGACATCATCAGTACAAGGAT ATCCCTAACCCATTAATTGCTGGAATAAGTAATGTACTAAATACCCCAAGCAGTGCAGCT TTTCCTACTGCATCTGCCGGAAGTAGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTC TCAGCAGCAGCCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGC AGCAGTAGCAATTCTGGAGCTGTTGCCGGCAGTGGCAACACTGAAGGACATAGCACTTTA AACACCATGTTCCCTCCTACTGCCAACATGCTTCTCCCAACAGGTGAAGGGCAAAGTGGT CGAGCAGCACTAAGAGATAAGCTGATGTCTCAGCAAAAAGACGCATTGCGGAAAAGAAAA CAACCACCTACGACAGTGTTGAGTTTGCTCAGACAGTCTCAAATGGATAGTTCTGCAGTT CCTAAACCTGGACCTGACTTGCTAAGGAAGCAGGGTCAGGGTTCATTTCCCATCAGTTCA ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACC CCGGGTTGTGGGGCCTCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTACA GATCCCAGTATGAACTCTAGTGTTCTTCAGAACATACCTTTAAGAGGGGAAGCCGTGCAC TGCCACAATGCAAACACTAACTTTGTTCACAGTAACAGTCCAGTCCCCAACCACCATCTT GCAGGTTTAATAAATCAGATTCAGGCTAGCGGGAACTGTGGGATGCTCAGTCAGTCGGGC ATGGCTTTAGGAAATTCCTTACATCCCAATCCACCTCAGTCAAGAATTTCAACGTCCTCC ACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACAAGTTCTGAAGCAGGC GGTTCAGGACCATCATCCTCCATAGCCATAGCGGGCACCAACCACCCTGCCATCACAAAG ACAACATCTGTTCTTCAAGATGGCGTCATAGTCACCACTGCAGCTGGAAACCCACTGCAG AGTCAGCTACCCATTGGGAGTGATTTTCCTTTTGTTGGCCAGGAGCACGCACTTCATTTT CCATCCAACAGCACTTCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGT TCTCTACCTATCTCTTTGCCAGTGAATCAACAGCATCTCCTAAACCAGAATCTATTAAAT ATCCTCCAGCCTTCAGCAGGAGAAGGTGATATGTCATCAATAAACAATACTTTGAGTAAC CATCAACTGACTCATCTACAGTCGCTGTTAAACAACAATCAGATGTTTCCTCCAAATCAG CAACAGCAGCAACTTCTCCAGGGGTACCAGAATCTCCAGGCGTTCCAAGGACAGTCCACA ATTCCTTGCCCAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTTCAGGTGAGA ATGCAGGAAGATGCAGCTCTCCTAAACAAAAGAATAAGCACTCAGCCTGGGCTCACAGCA CTTCCTGAGAATCCAAACACTACACTTCCACCTTTTCAAGATACACCTTGTGAGTTGCAA CCGAGGATTGACCCATCTCTTGGTCAACAGGTGAAGGATGGCCTCGTTGTGGGTGGCCCA GGTGATGCTTCCGTAGATGCCATTTACAAAGCAGTTGTCGATGCAGCCAGCAAAGGAATG CAGGTTGTCATCACCACTGCAGTCAACAGTACAACTCAGATCAGCCCCATTCCAGCTCTG AGTGCCATGAGTGCCTTCACTGCCTCAATTGGTGACCCATTAAATCTCTCCAGTGCTGTC AGTGCGGTCATTCATGGACGGAACATGGGAGGTGTTGATCATGATGGTAGGCTGAGGAAT TCAAGAGGGGCTCGGCTGCCCAAGAATCTAGACCATGGGAAAAATGTGAACGAAGGAGAT GGGTTTGAATATTTCAAGTCAGCAAGTTGCCACACATCCAAAAAACAGTGGGACGGGGAG CAAAGCCCCAGAGGGGAGCGAAACAGGTGGAAGTACGAGGAATTTTTAGATCATCCAGGC CATATCCACAGTAGTCCTTGTCATGAAAGGCCCAACAATGTCTCTACACTGCCATTTCTG CCTGGGGAACAGCACCCAATACTGTTACCACCAAGAAACTGTCCAGGGGATAAAATTCTA GAGGAAAATTTCAGGTATAATAACTACAAAAGAACTATGATGAGTTTTAAGGAGAGACTA GAGAACACTGTGGAAAGATGTGCACACATAAATGGGAATAGACCTCGACAGAGTCGGGGA TTTGGAGAGCTGCTAAGCACTGCAAAGCAAGACCTGGTCCTAGAGGAGCAGTCTCCAAGT TCCTCAAATAGTTTGGAAAATTCTCTGGTCAAAGACTACATCCATTACAATGGAGACTTT AATGCCAAAAGCGTTAATGGGTGTGTGCCTAGCCCTTCAGATGCTAAAAGCATTAGTAGT GAAGATGACCTANNGAACCCAGACTCCCCCTCTTCAAATGAATTGATACATTATAGACCA AGGACGTTCAATGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGCCT GGAAAATTAGTAAGAGAAGACGACGTTCACAATTCATGTCAACAAAGCCCCGAGGAAGGG AAGGTGGAGCCCGAGAAGTTGAAGACACTAACAGAAGGTTTGGAAGCCTACAGCCGTGTC CGGAAAAGGAACAGAAAAAGTGGAAAGCTAAATAACCATTTAGAAGCTGCTATTCATGAG GCCATGAGTGAACTGGACAAAATGTCTGGGACTGTACACCAAATCCCACAGGGTGACAGA CAAATGAGACCCCCCAAACCCAAGAGGAGGAAGATCTCCAGATAA Clone variation with respect to NM_018328.4 4093 a=>n;4094 g=>n >OriGene 5' read for NM_018328 unedited
TGCAAATTTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGTATGATTTTC CTTAGTCAGAAGCACTCATTTTTACCCATGTAGACCATCCTTAGGAATTAAATATTGGTT ATTTAATGTAGATTATAATGGGAAGCTGATTTTTTTCACAATGGCATATTTCAAGGACTT GGTTCCAAACTGAGCTGAAGCTTCCCAATGCGTTTTGTACAGTCTGGGAAAAACTGTGCT GCACTGGCCCACTTTTGAAGGCCATCATGCTCTGTAATATAAGGATATCATCTTATTGCT GATATCTTTGGAGAGTCCCTAGCAGACACAGAAAATGAATGGAGGCAAAGAGTGTGACGG AGGGGACAAGGAAGGAGGTCTTCCAGCTATACAAGTTCCTGTGGGTTGGCAGCGTCGTGT GGATCAAAATGGAGTGCTTTATGTCAGTCCCAGTGGGTCTTTGTTATCTTGCTTGGAGCA GGTTAAAACATACCTGCTTACTGATGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTAT TCTTCCCAAGGTATTTAATTTTGATCCTGGAGCTGCTGTGAAACAGAGAACCGCAGAAGA TGTTAAGGCAGATGAAGATGTCACAAAGCTATGCATACATAAAAGAAAAATTATTGCAGT GGCCACACTTCATAAAAGCATGGAAGCCCCACATCCTTCTCTGGTGCTCACCAGTCCCGG NAGANGAAACAAATGCACTNCAGTAGTACCTTCTCGGGCAGCAACTNCAAGATCAGTAAG AAATAAGTCTCATGAAGGAATTACANATNNCTGTATGCCTGAATGTAAGAATCCTTTCAA GTAATGATTGGATCATCAAATGCATGGGAAGGCTATTGTACAAAGACTGCCTGNAAGCCA ACAACAGAACTNCCCCTGTCTACCCCCGCAGAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018328 |
| Insert Size | 5200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018328.3, NP_060798.2 |
| RefSeq Size | 5325 bp |
| RefSeq ORF | 4485 bp |
| Locus ID | 55777 |
| UniProt ID | Q9P267 |
| Gene Summary | This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause an autosomal dominant type of cognitive disability. The encoded protein interacts with the polycomb repressive complex PR-DUB which catalyzes the deubiquitination of a lysine residue of histone 2A. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Jul 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Human Proteins MBD5 and MBD6 Associate with Heterochromatin but They Do Not Bind Methylated DNA
,null,
PLoS ONE
,PubMed ID 20700456
[MBD5]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222193 | MBD5 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 5 (MBD5) |
CNY 10,224.00 |
|
| RC222193L1 | Lenti ORF clone of Human methyl-CpG binding domain protein 5 (MBD5), Myc-DDK-tagged |
CNY 12,624.00 |
|
| RC222193L2 | Lenti ORF clone of Human methyl-CpG binding domain protein 5 (MBD5), mGFP tagged |
CNY 16,820.00 |
|
| RC222193L3 | Lenti ORF clone of Human methyl-CpG binding domain protein 5 (MBD5), Myc-DDK-tagged |
CNY 16,820.00 |
|
| RC222193L4 | Lenti ORF clone of Human methyl-CpG binding domain protein 5 (MBD5), mGFP tagged |
CNY 16,820.00 |
|
| RG222193 | MBD5 (tGFP-tagged) - Human methyl-CpG binding domain protein 5 (MBD5) |
CNY 16,440.00 |
