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POLR1B (NM_019014) Human Untagged Clone
CAT＃: SC113295
POLR1B (untagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1
CNY 7,912.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A135; RPA2; RPA135; Rpo1-2; TCS4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113295 sequence for NM_019014 edited (data generated by NextGen Sequencing)
ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACT GACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGG GCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCT ATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTTTACTATTCTGGATGCT GTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGAGGCCAATGTTTATCCA GCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGACAGCTGATATCAACTGG GCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTATGTTCCCATCATG GTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTCATTGAGCACCAT GAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAAAAAGTCATCCGA ATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAGACCAAAATGGAAAACC AGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGGGAAGAACATTCC GCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTGAACTTTATTTAC CGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTTGTCAGCTTTTCT GATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCTTTCCTTAGGAAC TCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACACAAAAACAGGTC CTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGACTGGTACCCAAAT GAGCAAGCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTGAAATCCAATACT GAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCTTTAGCCAAAGGAGAG TGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACACCGGGTCAGCTC TTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATTAAAATAGCTTTT GATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAATTTGATGAGGATTTTT ACAATGGGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACTGGGAATCTGCGT TCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGACTTTGTGTTGTGGCTGACAAGCTG AACTTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCTGATTTTGCCAAG ATGAGGACCACCACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTCCTTTGTCCCGTG CATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCCGTATGTGAGGTT GTCACACAGTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAACTTGGGGGTCACT CCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGCTACCCTGTCCTGCTGGACGGT GTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGATTCTCTTCGTCAT TTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTCCTTATACCCATG ACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCTTGTAGACTGGTA CGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACTATGGAACAGATC TTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACCACACACCAGGAA CTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTCTCTGATCACAAC CAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATGGGCTTTCCACTT CTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACTCCTCAGAGTCCC TTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCAATTGGGACCAAT GCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCCATGATTGTGAAT AAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCTGAGTTCATAGAC CTCTCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATCAAACCTGGTGAC CCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCGTTTATAGGAGCAAAACTGCAG TACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGTTTTGTGATGTAC TATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGTAATGACACTGGG AGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAACCCAACTATCGGA GATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTGTGGCCGGCTGAG GACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAATCCCCATGGTTTT CCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAGTCTGCAGCTTTG CATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAACTCGGCCTTAGAA TACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTATGGCACCGAGAGGTTATAT AGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTGGTTTATTATCAG CGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGAGCCCGAGACAGA GTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGTTTTGGGGAGATG GAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGACCGCCTCTTCAAC TGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTACTCTCTCCACTG TTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATACAACTGTACTCTG TGTAGTCGCAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTTCGGTATTTTGTA GCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTTTAA Clone variation with respect to NM_019014.4 >OriGene 5' read for NM_019014 unedited
GGCTTCGGATTTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGGCGTCCG GTGTGCAGGTGGCCACATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAG CCTAAAGCACTTGACTGACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTT GCAGGAGCTGACGCGGGCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGG CCTCGCGGTGCAGGCTATACCTCCCTTTGAATTTGCTTTCAAAGATGAGCGTATCTCTTT TACTATTCTGGATGCTGTTATCAGTCCACCTACAGTTCCAAAAGGGACCATCTGCAAAGA GGCCAATGTTTATCCAGCAGAATGCCGGGGCCGAAGGAGTACCTACCGTGGGAAGTTGAC AGCTGATATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGG CTATGTTCCCATCATGGTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGC CCTCATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCAT TGAAAAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAG ACCAAAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGT GAGGGAAGACATTCCGCTGTCAATATGAACCTCCACTACTTGGAANATGGCACTGGTTAT GTTGGAACTTTATTTANCCGANAGAACTGGTCTTTCTTTCTTTGGGATTTGCACTTAAGG CACTTGTCAGCTNTTNCTGATATCAGATCTTTCAGGAGCTCATCAAGGAAAAGAGGATGA TTCTTTCCTTAGAACTCTGTTCTC >OriGene 3' read for NM_019014 unedited
GACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCATCAAAAAACATC TTATAATTGCAGAAGTATATACAAGATGATCTATTATCAACAGTTTATAATGGCCAAAGA TAAGTCACCAGAATATTTGCAGAAAAAAGTTATAATGGCCAAAGAATGGAACAACTTAAA TACACAGGGGATGGTAAATCCATACAATAAAATACTGTACACAAGAAAGAAAACATAAGT GGCACTCTACACACTGCTATGAAAGCAGATCAATATAAAAGCAGAAAAAAGCCAAGTATG GAAGTGTAGACTAAATATAAAATACTCACCGGAGGCCAGGCACAGTGGCTCACACCCGTA ATCCTAGCACTTTCGGAGGCCGAGGTGGGTGGATCACTTGAGGTCAGGGTCTTCAAGACA GGGTCTTCCTATATTACCCAGGCTGTCCTTGAACTCCTGCACTCAAATGATCCTCCCACC TCAGCCTCTCAAATAGCAACGACTATAGATGCACAGCACCATGCCTGGCCTCCAAAATGG ATGAATCTTATTTCACTATGCTAAGTGAAATAAGCCAGTCACAGGACAAATATTGCATGA TCCCACTATATGAGATATCTGTAATAGTCAAACTCATACAAACAGGGTAACAAGGGTGGT TGCTAAGGCCCAGGGAAATGGACAGTTGTCCCACACGTATTAAGCTCTAGTTTGCCACAA CAAGCAGGTCTAAACATCTGTTGCCCACACTGGTTGCCTGCAATTAACCCCCTTATATCG CGCCTTTAAAAATTCCGTAAAAGGGTACGCTTCTTTTTTAACTCGCCCTTACCCCCCAAT AACTCCCATAAATCCTACAGCCCCTTTATTCATACAAAAATCGCGCCGCCCCCACTCTGC GCCCATCAAAGGTCTGTTATCTCCTCCTTTCCGCGGTGATGGTATCAAAGCGCCCGCCCT TTTCATCACCCCCCTATGTCTCTCTCCCCTTTATCAACTTCTATCTTCTCCCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_019014 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019014.3, NP_061887.2 |
| RefSeq Size | 5968 bp |
| RefSeq ORF | 3408 bp |
| Locus ID | 84172 |
| UniProt ID | Q9H9Y6 |
| Domains | RNA_pol_Rpb2_3, RNA_pol_Rpb2_5 |
| Protein Families | Transcription Factors |
| Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| Gene Summary | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220367 | POLR1B (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1 |
CNY 7,904.00 |
|
| RC220367L1 | Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC220367L2 | Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, mGFP tagged |
CNY 13,210.00 |
|
| RC220367L3 | Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC220367L4 | Lenti ORF clone of Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1, mGFP tagged |
CNY 13,210.00 |
|
| RG220367 | POLR1B (tGFP-tagged) - Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 1 |
CNY 9,504.00 |
