United States
Germany
Japan
United Kingdom
China
MCCC1 (NM_020166) Human Untagged Clone
CAT＃: SC113201
MCCC1 (untagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein
CNY 5,320.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MCC-B; MCCA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113201 sequence for NM_020166 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCAT CGTCTCCCGAGCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAG TACACAACAGCCACAGGAAGAAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATT GCCTGCAGGGTGATGCGCACAGCCAAAAAACTGGGTGTACAGACTGTGGCGGTTTATAGT GAGGCTGACAGAAATTCCATGCATGTAGATATGGCAGATGAAGCATATTCCATCGGCCCC GCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCT GCTGCACAGGCTATCCATCCAGGATGCGGTTTTCTTTCAGAAAACATGGAATTTGCTGAA CTTTGTAAGCAAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATG GGTATAAAGAGCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGT TATCATGGTGAGGACCAATCAGACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTAT CCTGTCATGATTAAAGCCGTCCGGGGTGGAGGAGGAAAAGGAATGAGGATTGTTAGATCA GAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGGAGAGAAGCTAAGAAGTCTTTCAAT GATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCATGTAGAAGTCCAGGTG TTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGG CGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAA AAGCTGGGAGAAGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACT GTGGAGTTTATTATGGACTCAAAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTG CAAGTGGAACATCCTGTTACTGAGATGATCACAGGAACTGACTTGGTGGAGTGGCAGCTT AGAATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAGAAATAACTCTGCAGGGCCAT GCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAATAACTTCATGCCTGTGGCAGGC CCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATTGAAACTGGAGTA CGGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTGTGG GCAGCAGATCGCCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATT GTTGGACTGCCCACCAACATTGACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAA GCTGGGAACGTGCACACTGATTTCATCCCTCAACACCACAAACAGTTGTTGCTCAGTCGG AAGGCTGCAGCCAAAGAGTCTTTATGCCAGGCAGCCCTGGGTCTCATCCTCAAGGAGAAA GCCATGACCGACACTTTCACTCTTCAGGCACATGATCAATTCTCTCCATTTTCGTCTAGC AGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACTCTTAAAGATGGTAAAAAC AATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAGATTGAAGAT AAAACTTTCCAAGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGT TCTGTTAATGGAGTTGCTAGTAAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTA TTTTCCAAGGAAGGAAGTATTGAGATTGACATTCCAGTCCCCAAATACTTATCTTCTGTG AGCTCACAAGAAACTCAGGGCGGCCCCTTAGCTCCTATGACTGGAACCATTGAAAAGGTG TTTGTCAAAGCTGGAGACAAAGTGAAAGCGGGAGATTCCCTCATGGTTATGATCGCCATG AAGATGGAGCATACCATAAAGTCTCCAAAGGATGGCACAGTAAAGAAAGTGTTCTACAGA GAAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAGGAGGAAGAATCAGAC AAAAGGGAATCGGAATAA Clone variation with respect to NM_020166.3 396 c=>t;1391 a=>c >OriGene 5' read for NM_020166 unedited
GGTTGTTCAGNNATATTTGTNAATACGCACTTCACTATAGNNGGCGGCCGCGCAATTCGG CACGAGGGCCCGCGGGGGTCACGAGGCTTCGTAGTGGAGGAACGGGTTTGGCGTGTGGGA CGCAGCTGCCTCTGTACTGGGGAGTCACGGAGTGGCCGGGCTCCAGGGACATGGCGGCGG CCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCATCGTCTCCCGA GCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAG CCACAGGAAGAAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGG TGATGCGCACAGCCAAAAAACTGGGTGTACAGACTGTGGCGGTTTATAGTGAGGCTGACA GAAATTCCATGCATGTAGATATGGCAGATGAAGCATATTCCATCGGCCCCGCTCCCTCCC AGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCTGCTGCACAGG CTATCCATCCAGGATGCGGTTTTCTTTCAGAAAACATGGAATTTGCTGAACTTTGTAAGC AAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATGGGTATAAAGA GCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGCACGGTTATCATGGT GAGGACCAATCAGACCAGTGCCTGGAGGAACACGCCAGNAGAATTGGCTATCCTGTCATG ATTAAAGCCGCCCGGGCTGGGAGGAAGGAAAATGATTGGACGCATTGGTTCCACCCCGAA ACAAGAATTTCTCAGAACCCGTTCACAGCCACGCACGGAAAGGAGCTTCAAAAACCTCTT TCAACGGATGACGCCTATGCCTGATTCGAAAAC >OriGene 3' read for NM_020166 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAAAAAAAAAAATTCACTTTTTTTATTTT AAAATTTTTGGAAAAAATTAGGGCCCCAAAGGCTTGATTTTCCAATTTAAAGGGGGTTCA CCTTAACCTTCCAATTTTTTAAAAAAACTGTTTTTTATAAAACCCCCAAAAAAGCGGGGG GCCTTTCTTTTTTTTGGGGGAAAAAAAAAACCTTTTTTAACGGGCCTTTTCTTTGGGGGA GTTAATCCCAATCCCCTTTGGGCGGATTTTTCCTCCCAAAACTCAATTAAAGGAGGGTGT TTTTTGGCCTAACCCCCTTTTTTGAAAAACATTTTTTTTACTGGGCCTTCTTTTGAAAAC TTTAGGGGAGCCCCCATTTTCGGGGGGATCTTAACCTTGGGGGAATCTCCCGTTTTCATT TTGTCCCCAGTTTTAAAAAACACCTTTTAAAGGGTTCCAACCATAGAACCTAAGGGGCCC CCCTGATTTTTTTGGGACCCCACAAAAAATAATTTTTGGGGGACGGGAATGCCAATCCCA ATACTTCCTTCCTTGAAAAAAGGAAAATAGGGTTTCCCAGAAAAAATCACTTTCTTTTTA CTACCAACTCCTTTAAAAAAACATTTAGGGTAAGGGCAGCCCCCCTCGCTGAAAAAATAC CAAGGCCTTGGAAAGTTTTATCTCAATTTGCTTGCTATAAAACCCCCAGGGTTATCCCTA CACCTATGGCTACTTGTTTTCCACATTTTTAAAAAACATGTTTCGGAGTAACAAGATATT AATTTTTTTTCACTGTGTAACAAAATGGGAAAATTTTCTTTTGTCCCGAAAAAGGAAAGT TCCGCCTTGGTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020166 |
| Insert Size | 2620 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020166.2, NP_064551.2 |
| RefSeq Size | 2528 bp |
| RefSeq ORF | 2178 bp |
| Locus ID | 56922 |
| UniProt ID | Q96RQ3 |
| Domains | biotin_lipoyl, CPSase_L_D2, CPSase_L_chain, Biotin_carb_C |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Leucine regulates autophagy via acetylation of the mTORC1 component raptor
,Son, SM;Park, SJ;Stamatakou, E;Vicinanza, M;Menzies, FM;Rubinsztein, DC;,
Nat Commun
,PubMed ID 32561715
[MCCC1]
|
|
Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A
,Son, SM;Park, SJ;Lee, H;Siddiqi, F;Lee, JE;Menzies, FM;Rubinsztein, DC;,
Cell Metab.
,PubMed ID 30197302
[MCCC1]
|
|
Selectivity in Post-translational Biotin Addition to Five Human Carboxylases
,Maria Ingaramo and Dorothy Beckett,
J. Biol. Chem., Jan 2012; 287: 1813 - 1822.
[MCCC1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200909 | MCCC1 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein |
CNY 5,312.00 |
|
| RC200909L3 | Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200909L4 | Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG200909 | MCCC1 (tGFP-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
