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SAR1 (SAR1A) (NM_020150) Human Untagged Clone
CAT＃: SC113196
SAR1A (untagged)-Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | masra2; SAR1; Sara; SARA1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113196 sequence for NM_020150 edited (data generated by NextGen Sequencing)
ATGTCTTTCATCTTTGAGTGGATCTACAATGGCTTCAGCAGTGTGCTCCAGTTCCTAGGA CTGTACAAGAAATCTGGAAAACTTGTATTCTTAGGTTTGGATAATGCAGGCAAAACCACT CTTCTTCACATGCTCAAAGATGACAGATTGGGCCAACATGTTCCAACACTACATCCGACA TCAGAAGAGCTAACAATTGCTGGAATGACCTTTACAACTTTTGATCTTGGTGGGCACGAG CAAGCACGTCGCGTTTGGAAAAATTATCTCCCAGCAATTAATGGGATTGTCTTTCTGGTG GACTGTGCAGATCATTCTCGCCTCGTGGAATCCAAAGTTGAGCTTAATGCTTTAATGACT GATGAAACAATATCCAATGTGCCAATCCTTATCTTGGGTAACAAAATTGACAGAACAGAT GCAATCAGTGAAGAAAAACTCCGTGAGATATTTGGGCTTTATGGACAGACCACAGGAAAG GGGAATGTGACCCTGAAGGAGCTGAATGCTCGCCCCATGGAAGTGTTCATGTGCAGTGTG CTCAAGAGGCAAGGTTACGGCGAGGGTTTCCGCTGGCTCTCCCAGTATATTGACTGA Clone variation with respect to NM_020150.4 >OriGene 5' read for NM_020150 unedited
GCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGA ATTCGGCACGAGGCCCGGGTGCTGCTGGTTAGTGTGCTCTGAGGGAGGGTCCGAGCCAGC CGCTGTTTTGCCGGAGGAGCCCCTCAGGCCGTAGTAAGCATTAATAATGTCTTTCATCTT TGAGTGGATCTACAATGGCTTCAGCAGTGTGCTCCAGTTCCTAGGACTGTACAAGAAATC TGGAAAACTTGTATTCTTAGGTTTGGATAATGCAGGCAAAACCACTCTTCTTCACATGCT CAAAGATGACAGATTGGGCCAACATGTTCCAACACTACATCCGACATCAGAAGAGCTAAC AATTGCTGGAATGACCTTTACAACTTTTGATCTTGGTGGGCACGAGCAAGCACGTCGCGT TTGGAAAAATTATCTCCCAGCAATTAATGGGATTGTCTTTCTGGTGGACTGTGCAGATCA TTCTCGCCTCGTGGAATCCAAAGTTGAGCTTAATGCTTTAATGACTGATGAAACAATATC CAATGTGCCAATCCTTATCTTGGGTAACAAAATTGACAGAACAGATGCAATCAGTGAAGA AAAACTCCGTGAGATATTTGGGCTTTATGGACAGACCACAGGAAAGGTGAATGTGACCCC TGAAGACCTGAATGCTCGCCCCATGGAAGTGTTCATGTGCAGTGTGCTCAAAAGCCAAGT TACGGCGAAGGTTCTCGCTGCCTCTCCCAGTATATTGACTGATGTTTGAACGTGGAAAAT AAAAAGTCCTACTTCTCTGGACTGAACCTATTCACACCTTCCTCATGAACTTTTTCTAAA AGACCAAGAAAGCTCTCCAACCCTGGCTTGGCGTTGAAAAGCCCAGAGTCTTTGGTAACT CTTTCATTGCCCCAAGTGTACAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020150 |
| Insert Size | 3200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020150.1, NP_064535.1 |
| RefSeq Size | 761 bp |
| RefSeq ORF | 597 bp |
| Locus ID | 56681 |
| UniProt ID | Q9NR31 |
| Domains | SAR, ARF, arf |
| Gene Summary | Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Insulin enhances posttranslational processing of nascent SREBP-1c by promoting its phosphorylation and association with COPII vesicles
,Chandrahasa R. Yellaturu, Xiong Deng, Lauren M. Cagen, Henry G. Wilcox, Charles M. Mansbach, II, Shadab A. Siddiqi, Edwards A. Park, Rajendra Raghow, and Marshall B. Elam,
J. Biol. Chem., Jan 2009; 10.1074/jbc.M805746200.
[SAR1A]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201450 | SAR1A (Myc-DDK-tagged)-Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 |
CNY 2,400.00 |
|
| RC201450L1 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201450L2 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC201450L3 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201450L4 | Lenti ORF clone of Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201450 | SAR1A (tGFP-tagged) - Human SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 |
CNY 4,000.00 |
