MEIS2 (NM_020149) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HsT18361; Meis1, myeloid ecotropic viral integration site 1 homolog 2; Meis1-related gene 1; Meis homeobox 2; Meis homolog 2; MGC2820; MRG1; MRG1, MGC2820, HsT18361; OTTHUMP00000160209; TALE homeobox protein Meis2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC113195 sequence for NM_020149 edited (data generated by NextGen Sequencing) ATGGCGCAAAGGTACGATGAGCTGCCCCATTACGGCGGGATGGACGGAGTAGGGGTTCCC GCTTCCATGTACGGAGACCCTCACGCGCCGCGGCCGATCCCCCCGGTTCACCACCTGAAC CACGGGCCGCCGCTCCACGCCACACAGCACTACGGCGCGCACGCCCCGCACCCCAATGTC ATGCCGGCCAGTATGGGATCCGCTGTCAACGACGCCTTGAAGCGGGACAAGGACGCGATC TATGGGCACCCGTTGTTTCCTCTGTTAGCTCTGGTCTTTGAGAAGTGCGAGCTGGCGACC TGCACTCCCCGGGAACCTGGAGTGGCTGGCGGAGACGTCTGCTCCTCCGACTCCTTCAAC GAGGACATCGCGGTCTTCGCCAAGCAGGTTCGCGCCGAAAAGCCACTTTTTTCCTCAAAT CCAGAGCTGGACAATTTGATGATACAAGCAATACAAGTACTAAGGTTTCATCTTTTGGAG TTAGAAAAGGTCCACGAACTGTGCGATAACTTCTGCCACCGATACATTAGCTGTTTGAAG GGGAAAATGCCCATCGACCTCGTCATTGATGAAAGAGACGGCAGCTCCAAGTCAGATCAT GAAGAACTTTCAGGCTCCTCCACAAATCTCGCTGACCATAACCCTTCTTCTTGGCGAGAC CACGATGATGCAACCTCAACCCACTCAGCAGGCACCCCAGGGCCCTCCAGTGGGGGCCAT GCTTCCCAGAGCGGAGACAACAGCAGTGAGCAAGGGGATGGTTTAGACAACAGTGTAGCT TCACCTGGTACAGGTGACGATGATGATCCGGATAAGGACAAAAAACGCCAGAAGAAAAGA GGCATTTTCCCCAAAGTAGCAACAAATATCATGAGAGCATGGCTCTTCCAGCATCTCACA GTTTATTAA Clone variation with respect to NM_020149.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_020149 unedited TTTGGGCGGTTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAAAG AAGAGAAAAAGAGAAAAAGAGAATATCACAGAAAAAAAATTCTTCGTTGTCTAGACTGGG CTTTTTTTTCCCCCCTAAAAAATAGCATATTGGAGAATTGGGAGAAGTCTCTTTGGTTTG GAAAAAAAAAAAAGGAATCTTCAGCCTAGATCACTTTCTTATCCGGACTGGGATATTAAA TATACGACACATCCAGGAGTTTATTGGAGCGCAGACTGATGGCGCAAAGGTACGATGAGC TGCCCCATTACGGCGGGATGGACGGAGTAGGGGTTCCCGCTTCCATGTACGGAGACCCTC ACGCGCCGCGGCCGATCCCCCCGGTTCACCACCTGAACCACGGGCCGCCGCTCCACGCCA CACAGCACTACGGCGCGCACGCCCCGCACCCCAATGTCATGCCGGCCAGTATGGGATCCG CTGTCAACGACGCCTTGAAGCGGGACAAGGACGCGATCTATGGGNCACCCGTTGTTTCCT CTGTTAGCTCTGGTCTTTGAGAAGTGCGAGCTGGCGACCTGCACTCCCCGGGAACCTGGA GTGGCTGGGGGNAGACGTCTGCTCCTCCGACTCCTTCAACGAGGAATTTGGCGGTCTTCG CCAAGCAGGTTCGCGCCCGAAAGCCACTTTNTTCCTCAAATCCAGAGCTGGACAATTTGA TGATACAAGCAATACAGTACCTTAGGTTTCATCTTTTTGGAGTAGNAANAAGGTCCACGA ACTGTGCGATAACTTCTGCCCCGATCTTTTAGCTGNTTTGAAGGGGAAAATGCCATCGAC CTCGTCATTGATGAAAGAGACGGCAGCTCAAGTCAATCATGAAAAAACTTCAGGGCTCCC ACAATCTCGCTGAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_020149 unedited GCTTTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTCAAAAAT GAAAATTTATTACCGCTTTTTGTGACTTAACACCTTTTTTTTTTAACATAACGTCACAGT CCTCATACAAGTATTTTAATGTAAATTTGACAAAGCTTAAAGGTAACAGCATTTTCTTCT AGTGAGGAACACGTGCTGAGAAAAGAAGAATTCATGGACATACAATACCAATTCCACAGC AGATCTGATACTAGCAAAAACATTCTTTTTTTTTTCAATTGAGGTAAACACATAGAATAT CTAACATGAAACAATTAATAGACCGAACTCTGTACGAAGTTTGTTACAGTATTCTCTTGC TCCTTTTTATCCCCCAAGCTTTGAGTTTCTGATAAAGTCCTAGTTATGGTTCAATGACCA TTAATAACTTTTTTTGTGTTGAGGAAAGCTGCCCAACTTAAGATTGTTTTGTCCACAACC AAGGCTCAAAACTCCTGGAAGAAGCTCCTGCTCTGTCTTTAAATCTTCATTGGCAAGCTC TTTGAAGAAGAGTCCCCCAAAAATAATAATAAGAATTGGCTTATGAAGCACGAACTATAA AGATCTGTTTGAAACTAAAGGACACATTTATGGAGGAACAAAGGCCGGGCATGAAAACAC ATTCTTGAAGCTGGAGTTCGAGTGATGAGGTTCAGCCAAAAGTTCATCATGATCTCCAGT GTGGTTCTTTTCTCATTGGCCCAACAGGTTTAAAATATATACCACAGTCTCTCCTGGATG GTGAATCACTGATGAACCCAACAGTCATTCTTTTGGAAACACCACATAGTGTGGAAACAG GTTATATNTTTTTTTCTCTCTAAACTATTGAGGCACATACGGCGGATCACAGAAGTACAG NTAACTTAGTCCTATTTATCTACGAGTATACTCTGGACTTTTTCAAGATCTACTGGACGA TGCTCATCACCT
Restriction Sites	NotI-NotI
ACCN	NM_020149
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020149.2, NP_064534.1
RefSeq Size	3623 bp
RefSeq ORF	909 bp
Locus ID	4212
Protein Families	Transcription Factors
Gene Summary	This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (e) has multiple differences in the coding region, compared to variant a, one of which results in a frameshift. The resulting isoform (e) has a shorter and distinct C-terminus, compared to isoform a.

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