G0 Protein alpha (GNAO1) (NM_020988) Human Untagged Clone
CAT#: SC113061
GNAO1 (untagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1
CNY 3,656.00
CNY 5,700.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DEE17; EIEE17; G-ALPHA-o; GNAO; HLA-DQB1; NEDIM |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC113061 sequence for NM_020988 edited (data generated by NextGen Sequencing)
ATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAG AAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGG GCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGC TTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCC CTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGA AAGGCTGACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTC TCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGC TTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTG GATCGGATTGGGGCCGCCGACTACCAGCCCACTGAGCAGGACATCCTCCGAACCAGGGTC AAAACCACTGGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTT GACGTCGGAGGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACG GCCATCATTTTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACC ACGAACCGCATGCACGAGTCTCTCATGCTCTTCGACTCCATCTGTAACAACAAGTTCTTC ATCGATACCTCCATCATTCTCTTCCTCAACAAGAAAGATCTCTTTGGCGAGAAGATCAAG AAGTCACCTTTGACCATCTGCTTTCCTGAATACACAGGCCCCAATACCTATGAAGACGCA GCCGCCTACATCCAAGCACAATTTGAAAGCAAAAACCGCTCACCCAACAAAGAAATATAT TGTCACATGACTTGTGCCACAGACACGAATAACATCCAGGTGGTGTTCGACGCCGTCACC GACATCATCATTGCCAACAACCTCCGGGGCTGCGGCTTGTACTGA Clone variation with respect to NM_020988.2 513 c=>t >OriGene 5' read for NM_020988 unedited
TCANGATATTTGTATACGACTTACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGGCGG GCTGTGGCAGGGAAGGGGCCACCATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCC TCGAGCGGAGCAAGGCGATTGAGAAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAG ACGTGAAATTACTCCTGCTCGGGGCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGA TGAAGATCATCCATGAAGATGGCTTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTG TCTACAGCAACACTATCCAGTCCCTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCA TCGAATATGGTGATAAGGAGAGAAAGGCTGACGCCAAGATGGTGTGTGATGTGGCGAGTC GGATGGAAGACACCGAGCCCTTCTCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGG GCGACTCAGGAATCCAAGAGTGCTTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTG CCAAATACTACCTGNACAGCCTGGATCGGATTGGGGCCGCCGACTACCAGCCCACTGAGC AGGACATCCTCCGAACCAGGGTCAAAACCACTGGCATCGTAGAAACCCACTTCACATTCA AGAACCTTCACTTCAGGCTGTTTGACGTCGGAGGCCAGCGATCTGAACGCAAGAAGGGAT CCATTGCTTCGAGGACGTCACGGCCATCATTTCTGTGTCGCGCTCACCGGCTATGACCCA GTGCTCCACGAAGACCAAACCCCAACCCGCATGCCGAGTCTCTCATGCTCTTCGACTCCT CTGAACAACAAGTCCTCATCGATACCTCATCATTCTCTTTCTCAACAGAAAGATCCCTCT GCCGAGAGAACCAGAAGTCN >OriGene 3' read for NM_020988 unedited
ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGTGTTTTGTCTTTTT GCCTCCTCTTTATTTGAAATGGCTGTGACTGGTCTTCTTCAGCAAAGATTAAGTCTTAAA AGTGAAACCGTGAAGAGGTTTAGGGTTTGGGTATTAGTCAAAGTAGAAATGACATAGGAT TGGTCATGAAAACGGCGGCTTTAATGTAAACACTACATTCCATCTTTTTAAATTTTTTTG TTTTTTTTTTCCATCTTGTGGTATTTAAAAATTTTTTTTTGTTCTTTTTGTCTGCAGCAC AAACATCCCCACATGAGAGAGCGGACACAGGGCACCGAAACAAAGGCAGGGGTGGGGAGG GGCGCACACACGAAGGGAGGTGTCAGCCGGGACCGGAAATCCAACACGGCAAAGGAAAAA AAACACAACCCGTTTCCCAAAGACTAGGAAAACTGATCTGTGGGGTGGGGGGCTGGTAAT ACATAGTACTCGGCGGGACAAAAGTAAAAACAAAAACTGAAAAAAAAAAAAAAGGAACCA AAAAACCCCTACAAAGGCTATCCTTANAAAGGGGGTCGGTGGGCANATTGTTTTCTATGG GGCTGGATTTCCTTATACGATGGGTGTTCTTTTTGGCAGCTACTGGCCAGTGAGAGCTGT GCTTTTTCTATACCAACCTGGAGTGANCGTGTGGCGGGATGGCTTANGGCCCCCACAGTG CCTCTGTGGTGTCTGGGCCGCCCGCAGGCACCAGGGGTTGCAGTGGGCCTGGGGCCTTCT GGAAAAGCCCGCCCGAGCCCACTCCTTCGACCTTGCACCCGACCGGAACCCCAGCGGCAG ATGGGCCCTGCCCCCACAGAACTTCAGGGCTTCCCCCAAAGGGGAACGGGCCAGCCCCGG CCAATAGGTTTTTTTTTTCAAAC |
Restriction Sites | NotI-NotI |
ACCN | NM_020988 |
Insert Size | 2750 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_020988.1, NP_066268.1 |
RefSeq Size | 2215 bp |
RefSeq ORF | 1065 bp |
Locus ID | 2775 |
UniProt ID | P09471 |
Domains | G-alpha |
Protein Families | Druggable Genome |
Protein Pathways | Long-term depression, Melanogenesis |
Gene Summary | The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the shorter transcript and encodes isoform a. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217958 | GNAO1 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1 |
CNY 3,656.00 |
|
RC217958L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC217958L4 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG217958 | GNAO1 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 1 |
CNY 5,256.00 |
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