POLD4 (NM_021173) Human Untagged Clone
CAT#: SC112993
POLD4 (untagged)-Human polymerase (DNA-directed), delta 4 (POLD4)
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | p12; POLDS |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC112993 sequence for NM_021173 edited (data generated by NextGen Sequencing)
ATGGGCCGGAAGCGGCTCATCACTGATTCCTACCCGGTTGTGAAGAGGAGGGAGGGGCCC GCTGGGCACAGCAAGGGGGAGCTGGCACCCGAGCTAGGGGAGGAGCCCCAGCCCCGCGAC GAGGAGGAAGCGGAGCTGGAGCTGCTGAGGCAGTTTGACCTGGCCTGGCAGTACGGGCCC TGCACCGGGATCACACGGCTGCAGCGCTGGTGTCGGGCCAAGCAGATGGGCTTGGAGCCT CCCCCAGAGGTGTGGCAGGTGCTGAAGACCCACCCCGGAGACCCCCGCTTCCAGTGCAGT CTCTGGCATCTCTATCCCCTATGA Clone variation with respect to NM_021173.3 >OriGene 5' read for NM_021173 unedited
GTCAGAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCTGCCCGC CGCTGGCCTGGCCCCGTCTGTCTCTCTCAGCAGCTGTCTTTCTCGCGCCCACTGGCCGGT CTCTCCTCTTCCCCGCAGTTGCCTCCTTCTCTGCCTGCCTGGGTGGCCGCCATGGGCCGG AAGCGGCTCATCACTGATTCCTACCCGTTGGTGGAAAGAGGAGGGAGGGGCCCGCTGGGC ACAGCAAGGGGGAGCTGGCACCCGAGCTAGGGGAGGAGCCCCAGCCCCGCGACGAGGAGG AAGCGGAGCTGGAGCTGCTGAGGCAGTTTGACCTGGCCTGGCAGTACGGGCCCTGCACCG GGATCACACGGCTGCAGCGCTGGTGTCGGGCCAAGCAGATGGGCTTGGAGCCTCCCCCAG AGGTGTGGCAGGTGCTGAAGACCCACCCCGGAGACCCCCGCTTCCAGTGCAGTCTCTGGC ATCTCTATCCCCTATGAGGCACCACGTAAGACCTCCTGCCCTTAGCTCTCTTGCTTACCA CCCAAGAACCTCAGGACAGAAGCGAGAGCCCATTGCTCCTGCTCAGCTCAGCCCGGCTGC GGAGGAACCCTTGGCAGGCAGAACCTGGAGGTGTCAGGCTCAACTCCTCCATCTAACCAG CAGGCTCCCAGAGTCCCCGGAAGAGCCTGCGCAGCTGAAGCAGAGTGCTTCTAGAATGGA GAGTGGTCACTNGGGGAAAAGGACCTGGCCATCACCTTNCAATACCTGCTGCCTGTCNTC CTGACCCATGATCTGGNCAGTTAGGCACAGTCAGACATGGACAGTTGATCCATGAGGAAA AGATGCTCTCCCACCTAAGGCCCAGAATCTGAAGCCAGACTGGCTGAGCTCCAGGNCCAA GGGGTTCACTTATGCCTATCANTAAGGATAATGGAGCCTTGGAAAAAAAAAAAAAAAAAC TACCTANATGGGGGCCGGGTTAAATTGTT >OriGene 3' read for NM_021173 unedited
ACCGCGAAACCCANCTTAGATCGAGTTTTTTTTTTTTTTTTTTTTTTTCCCGGCCTCAAT ATTCTTTATTGATAAGCATTAGTGAACCCCTTGCCCTGGGAGCTCAGCCAGTCCTGCTCT CAAATTCCTGGCCTTAGGTGGGAGAGCATCTTTTCCTCATGGATCAACTGTCCATGTCTG ACTGTGCCTAACTTGCCAAATCATGGGTTGGTAGACAGGCAGCAGGTATTGGAAGGTGAT GGCCAGGTCCTTTTCCCCAGTGACCACTCTCCATCTAAAAGCACTCTGCTTCAGCTGCGC AGGCTCTTCCGGGGACTCTGGGAGCCTGCTGGTTAAATGGAGGAGTTGAGCCTGACACCT CCAGGTTCTGCCTGCCAAGGGTTCCTCCGCAGCCGGGCTGAGCTGAGCAGGAGCAATGGG CTCTCGCTTCTGTCCTGAGGTTCTTGGGTGGTAAGCAAGAGAGCTAAGGGCAGGAGGTCT TACGTGGTGCCTCATAGGGGATAGAGATGCCAGAGACTGCACTGGAAGCGGGGGTCTCCG GGGTGGGTCTTCAGCACCTGCCACACCTNTGGGGGAGGCTCCAAGCCCATTTGCTTGGCC CGACCCAGCGCTGNAGCCGTGTGATCCCGGTGCATGGCCCTGTACTGCCAGGCCCCGGCC CCCCTGTCTTAAGAGTTCCCGCTTCCCTTCTTCCTCGCCGCGCGGTCGGGGGTCTTTCCT CTACCTCGGCGGCCCCTCCCCGCCTTGTTTCCCCACCTGTTTCTCTCTCCCTTTTTCTCC CCCCTCGCTCCCCCCCCCGCCATATCCCCTTCTGGCCTTTCCCGTCTTCCCCTCCGCCCC CCCCCCGCCCCCCCCTCTTTTTCCCCCCCCCCTCCCCTTTCCCCCCCCCTCTCGTCCCCC TCCTCCCTCCTCCTCTTCTGCCCCTCTTCCCCCCCCCCCCCCCCCGCCTTTTTCCCCCCT TTTCTTTCCTTCCCCCACTCACTTCTAAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_021173 |
Insert Size | 930 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_021173.2, NP_066996.2 |
RefSeq Size | 946 bp |
RefSeq ORF | 324 bp |
Locus ID | 57804 |
UniProt ID | Q9HCU8 |
Domains | DNA_pol_delta_4 |
Protein Pathways | Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
Gene Summary | This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209992 | POLD4 (Myc-DDK-tagged)-Human polymerase (DNA-directed), delta 4 (POLD4) |
CNY 1,200.00 |
|
RC209992L3 | Lenti ORF clone of Human polymerase (DNA-directed), delta 4 (POLD4), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209992L4 | Lenti ORF clone of Human polymerase (DNA-directed), delta 4 (POLD4), mGFP tagged |
CNY 5,890.00 |
|
RG209992 | POLD4 (tGFP-tagged) - Human polymerase (DNA-directed), delta 4 (POLD4) |
CNY 2,800.00 |