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MASA (ENOPH1) (NM_021204) Human Untagged Clone
CAT＃: SC112927
ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1)
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | E1; MASA; MST145; mtnC |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112927 sequence for NM_021204 edited (data generated by NextGen Sequencing)
ATGGTCGTGCTTTCGGTCCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACC ACAACCCCGATTGCTTTCGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAA GAGTATCTGCAGACACATTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGG AAACAGGCTGAAGAGGACGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGG AATGGAGTGGATGATCTGCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAG ATGTCCCTGGATCGAAAGACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCG GCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCAGG AAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAGGCACAG AAACTGTTATTCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTT GATACCAAGATTGGACACAAAGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATT GGGTGCTCAACCAACAACATTTTGTTTCTGACAGATGTTACTCGAGAGGCCAGTGCTGCT GAGGAAGCAGATGTGCACGTAGCTGTGGTGGTGAGACCAGGCAACGCAGGATTAACAGAT GATGAGAAGACTTACTACAGCCTCATCACATCCTTCAGTGAACTATACCTGCCTTCCTCA ACCTAG Clone variation with respect to NM_021204.3 >OriGene 5' read for NM_021204 unedited
AGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGGTGTGCAG AAGTGTCCTCTCCCCACGCGCGGCGGGCTGCACTTGGTCGCTGGCTCCGAGATCGCGCGG GGCCGCCGGAAGCCCAAGACGGTACCGGGGGCCGCAGCCGCAGCCGGCGCCGCCCTCCGC CCTCCCCAACAGCAGGCCGAGTCCCGTAGCATCCGGTAGGGAAATGGTCGTGCTTTCGGT CCCCGCCGAAGTCACCGTGATCCTGTTAGATATCGAAGGTACCACAACCCCGATTGCTTT CGTGAAGGACATTTTATTTCCTTACATCGAAGAAAATGTTAAAGAGTATCTGCAGACACA TTGGGAAGAAGAGGAGTGCCAGCAGGATGTCAGTCTTTTGAGGAAACAGGCTGAAGAGGA CGCCCACCTGGATGGGGCTGTTCCTATCCCTGCAGCATCTGGGAATGGAGTGGATGATCT GCAACAGATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAA GACCACTGCACTCAAACAGCTGCAGGGCCACATGTGGAGGGCGGCATTCACAGCTGGGCG CATGANAGCAGAGTTCTTTGCAGATGTAGTTCCAGCAGTCANGAAGTGGAGAGAGGCCGG AATGAANGTGTACATCTATTCCTCAGGNAGTGNTGANNGCACAGAAACTGNNTATTCGGC ATTTCTACGAGGGAGATATTTCTTGAGCTGTTGATGGTCACTTTGATACCCAGATTGGAC ACANAGTAGAGAGTGAAAGTTACCGAAAGATGCAGACAGCATTGGGTGCTCAACCACCAC ATTTTGTTTCTGACAGATGTTACCTCCAAAGGCCAGTGCTGCTGAAGAAGCAAATGTGCA CGTAGCTGTGGTGGTGAAACCAGGCACCGCAGATTACAGATGATGAGA >OriGene 3' read for NM_021204 unedited
NNNATTTTTTAGCTTGNACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTT TGAAGAAAAAAGCTTTTATTAATCACAACATGAAATTAACATGACATCGTATTAGAATTA AACCAGGATAAATACTGCTGTTATAAAATTTACATTTCTCACATCCATTCCAGAGGAAAA TCTTACATATTAGGGCTCATCTTAATGTTATGGACTGATTTCAGTAAAACTTTTTAAATA GTAAATAGCAATTAACGTATTCTCAAACTGTGCTAAGTAGTTAGAAAGGCAACTATAAAA TCTATAATGATAACACGTGGCAGGATTAGAACTGTTCTGTTAAACATTTAAGAACCACAT CTTTGTTTTAGATGTTACCTCAGTGCACCTTTTTCACTGGTAAGGCACCTGCTTGCTGTG GGTGCCCCATTGTTACCCAGGTTTCTTTTTAGTAAAAACCCTTGGTTGAACAAACCTAAG GGAGTTTTCTTTCCCATTTTGGCCTTTGAACCGAAAGGAAACCCTGGGCCTTGGTTTTTT AAGGGGGCCATTTGAAACCCCCTGTTTAATTTTTTAATCCACGGGGCCTAAAATTTCCTT TCTTTTAAAAAATTCCCCTTTAAAAATTTTATTTTTAAAAAAACGGGCCTTAAAAAAACC TCAAACCCCCTTTCCCCTTTTCCCCCCCTTTTTAACCCTCCCCCCCCTAAAGGGGTTTTA AAATTTTTTTCACCCACCCCCTTTCCCCCCCTTTGGGGGGAGAAAGATTTTAAAAAAATT TCCCCCAAAAAAACCTTTTAAAAAAATTGTGTGGCCCCCAAAAAAAATTTTTTTTTTTTT ATCCCCGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021204 |
| Insert Size | 1990 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021204.1, NP_067027.1 |
| RefSeq Size | 1992 bp |
| RefSeq ORF | 786 bp |
| Locus ID | 58478 |
| UniProt ID | Q9UHY7 |
| Domains | Hydrolase |
| Protein Pathways | Cysteine and methionine metabolism |
| Gene Summary | Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Role for human arylamine N-acetyltransferase 1 in the methionine salvage pathway
,Witham, KL;Minchin, RF;Butcher, NJ;,
Biochem. Pharmacol.
,PubMed ID 27865712
[ENOPH1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209510 | ENOPH1 (Myc-DDK-tagged)-Human enolase-phosphatase 1 (ENOPH1) |
CNY 2,400.00 |
|
| RC209510L3 | Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209510L4 | Lenti ORF clone of Human enolase-phosphatase 1 (ENOPH1), mGFP tagged |
CNY 5,890.00 |
|
| RG209510 | ENOPH1 (tGFP-tagged) - Human enolase-phosphatase 1 (ENOPH1) |
CNY 4,000.00 |
|
| SC321962 | ENOPH1 (untagged)-Human enolase-phosphatase 1 (ENOPH1) |
CNY 2,400.00 |
