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SLC22A3 (NM_021977) Human Untagged Clone
CAT＃: SC112840
SLC22A3 (untagged)-Human solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SLC22A3)
CNY 6,224.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EMT; EMTH; OCT3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_021977 edited
ATGCCCTCCTTCGACGAGGCGCTGCAGCGGGTGGGCGAGTTCGGGCGCTTCCAGAGGCGC GTGTTTTTGCTGCTGTGCCTGACGGGCGTCACCTTCGCCTTCCTCTTCGTCGGCGTGGTC TTCCTGGGCACGCAGCCCGACCACTACTGGTGCCGCGGGCCAAGTGCCGCGGCGCTGGCC GAGCGCTGCGGCTGGAGCCCGGAGGAGGAGTGGAACCGCACGGCGCCCGCCTCCCGCGGC CCAGAGCCCCCCGAGCGCCGCGGCCGCTGCCAGCGCTACCTCCTGGAGGCGGCCAACGAC AGCGCCTCCGCCACTAGCGCTCTCAGCTGCGCGGACCCACTCGCCGCCTTCCCCAACCGC TCGGCTCCCCTTGTGCCGTGCCGCGGCGGCTGGCGCTACGCCCAGGCCCACTCCACCATC GTCAGCGAGTTTGACCTTGTCTGTGTCAATGCGTGGATGCTGGACCTCACCCAAGCCATC CTGAACCTCGGCTTCCTGACTGGAGCATTCACCTTAGGCTATGCAGCAGACAGGTATGGC AGGATCGTCATTTACTTGCTATCCTGCCTTGGTGTTGGCGTCACTGGGGTTGTGGTGGCC TTTGCACCAAACTTCCCTGTGTTTGTGATCTTCCGCTTCCTGCAAGGTGTATTTGGAAAG GGGACGTGGATGACTTGCTACGTGATTGTGACAGAAATAGTAGGTTCGAAACAAAGGAGG ATTGTGGGAATCGTGATTCAAATGTTCTTTACCCTTGGAATCATAATTCTCCCTGGAATT GCCTACTTCATCCCCAACTGGCAAGGAATCCAGTTAGCCATCACGCTGCCCAGCTTTCTC TTCCTCCTTTATTACTGGGTGGTCCCTGAGTCTCCCCGTTGGCTGATTACTCGGAAGAAA GGAGATAAAGCATTACAGATCCTGAGACGCATTGCTAAGTGCAATGGGAAATACCTCTCA TCAAATTACTCAGAGATCACTGTTACAGATGAGGAAGTTAGTAATCCATCCTTTTTAGAT CTGGTGAGAACTCCCCAAATGAGGAAATGCACACTTATTCTTATGTTTGCTTGGTTCACA AGCGCAGTGGTGTATCAAGGACTTGTCATGCGCCTGGGAATTATAGGGGGCAACCTCTAT ATAGACTTTTTCATCTCGGGCGTGGTGGAACTGCCAGGAGCTCTCTTGATCTTACTAACC ATTGAGCGCCTTGGACGACGCCTCCCCTTTGCAGCAAGCAATATAGTGGCAGGGGTGGCA TGCCTTGTCACTGCGTTCTTACCAGAAGGAATAGCATGGTTGAGGACCACAGTGGCTACA TTGGGAAGACTAGGGATAACCATGGCCTTTGAAATTGTTTATTTGGTAAATTCAGAATTG TACCCAACAACATTACGAAATTTCGGAGTTTCGCTCTGTTCAGGTCTGTGTGATTTTGGG GGAATCATAGCCCCATTTCTGCTCTTTCGGCTAGCAGCCGTGTGGCTAGAACTACCTCTG ATCATCTTTGGTATCCTGGCATCCATCTGTGGTGGCCTTGTGATGCTTTTGCCTGAAACC AAGGGTATTGCCTTGCCAGAGACAGTGGATGATGTAGAAAAACTTGGCAGTCCACATTCC TGTAAATGTGGCAGGAATAAGAAAACCCCAGTTTCCCGCTCTCACCTTTGA >OriGene 5' read for NM_021977 unedited
GGATTTTGGTATACGACTCACTATAGGGCGGCCCGCGAATTCGCACCAGGCGGGCTGCGG GCGGCGGGCGGCGGGCGCACCATGCCCTCCTTCGACGAGGCGCTGCAGCGGGTGGGCGAG TTCGGGCGCTTCCAGAGGCGCGTGTTTTTGCTGCTGTGCCTGACGGGCGTCACCTTCGCC TTCCTCTTCGTCGGCGTGGTCTTCCTGGGCACGCAGCCCGACCACTACTGGTGCCGCGGG CCAAGTGCCGCGGCGCTGGCCGAGCGCTGCGGCTGGAGCCCGGAGGAGGAGTGGAACCGC ACGGCGCCCGCCTCCCGCGGCCCAGAGCCCCCCGAGCGCCGCGGCCGCTGCCAGCGCTAC CTCCTGGAGGCGGCCAACGACAGCGCCTCCGCCACTAGCGCTCTCAGCTGCGCGGACCCA CTCGCCGCCTTCCCCAACCGCTCGGCTCCCCTTGTGCCGTGCCGCGGCGGCTGGCGCTAC GCCCAGGCCCACTCCACCATCGTCAGCGAGTTTGACCTTGTCTGTGTCAATGCGTGGATG CTGGACCTCACCCAAGCCATCCTGAACCTCGGCTTCCTGACTGGAGCATTCACCTTAGGC TATGCAGCAGACAGGTATGGCAGGATCGTCATTTACTTGCTATCCTGCCTTGGTGTTGGC GTCACTGGGGTTGTGGTGGCCTTTGCACCAAACTTCCCTGTGTTTGTGATCTTCCGCTTC CTGNCAGGTGTATTTGGAAAGGGGACGTGGATGACTTGCTACGTGATTGTGACAGAAATA GTAGGTTCGAAACCAGGAGGATTGTGGGAATCGTGATTCAAATGTTCTTTACCCTTGGAA TCATATTCTCCCTGGNAATGCCTACTNCATCCCCACTGGCAGGNAATNCAGTTAGCCATC ACGCTGNCCAGCTTTCTCTTTCTCCTTTATACCTGGNNGGNNCCCTGAGTCTNCCCGTTG GCCTGATACTCCGAA >OriGene 3' read for NM_021977 unedited
CCCCCATTCNTTGCACCGCGGCCGCATTCTAGACNCGGCTTTTTTTTTTTTTTTTTTACG ATTTAAAATTTTAATTGTTACCAAACAAAAAATCCACTCAAAATACAATTCAACAATGCA ACAGTCATCTTACAGCAGAGAAATGCATGAGAAAAGCAAAACTGCAAGTGACTGTGAATA AAGGGTGAATGTAGTCTCAAATCCTCAAAGAGTTGTGTTTATTTCATCGACAAATAGATT ATTCGTATTCAATTCTGATGTGTTTTAAAGACTAAGATGCTCATTTTACGATTAGCGCAC ATGTGTATATTGTCACCTGTTCTCCTTAGAAAAATGCTCTTCCTCATGGTTAATGTTTCC CTTTGGTACCAATTTTGCCTGTTTGCATAAAGTATTTCATTTTTAAAACTCAGCATAAAA TATAGACTGCATCTGAAAAGATATTGAATATAACATATAAAGCAGAACACACAAGTTCCA CATTTCAAAAGCCTCGATAACAAAAAAAGAAAGATCAAATCCCTTATTAGTCTCTGTAAA GTCATTTATAAAGCTCCTGCTGATCCTGTTAAGCATGGATATAATTGATCTTTCTTTTAA ATTAAAATGTAAGCTTTTCCCCAAGACATGTCAAAGTGCTAAGAATGAATAGGAATTGTC CATGTTTTTCGCTTTTGACCACGGGCACCATCGGAACTGAACTCACAAATATAAGAAAAA TATCCCTTCACTTTTAAAATCCTAAGACCCAGAAATCAGAATCCATGGGATTTGTTTAGT ACAGCCAGGAATCTGTGGCTCTAGGTGTTCACTACAGGAGATCAAAAATGTCCCAAATAA TCTCAGCACCACAGCATCATTTTGTAAATACAGAGCCAATCCTCAGATGTCCGCAGCATG GTCCTAATGCTGGCACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021977 |
| Insert Size | 3340 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021977.2, NP_068812.1 |
| RefSeq Size | 5624 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 6581 |
| UniProt ID | O75751 |
| Domains | sugar_tr |
| Protein Families | Transmembrane |
| Gene Summary | Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Significance of OCT3/SLC22A3, Organic Cation Transporter 3, Expression for the Cytotoxic Effect of Oxaliplatin in Colorectal Cancer
,Sachiko Yokoo, Satohiro Masuda, Atsushi Yonezawa, Tomohiro Terada, Toshiya Katsura, and Ken-ichi Inui,
Drug Metab. Dispos., Aug 2008; 10.1124/dmd.108.023168.
[SLC22A3]
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Other Versions
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| RC218172L3 | Lenti-ORF clone of SLC22A3 (Myc-DDK-tagged)-Human solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SLC22A3) |
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| RC218172L4 | Lenti-ORF clone of SLC22A3 (mGFP-tagged)-Human solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SLC22A3) |
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| RG218172 | SLC22A3 (tGFP-tagged) - Human solute carrier family 22 (extraneuronal monoamine transporter), member 3 (SLC22A3) |
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