POLR2H (NM_006232) Human Untagged Clone
CAT#: SC112792
POLR2H (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H)
CNY 1,200.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RPABC3; RPB8; RPB17 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_006232 edited
ATGGCGGGCATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAG AAGTTTGACCGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATC TTAGATGTAAACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATA GCTAGTACCTTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGAT AGGCCTTCCAGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAG GGAGATGAAACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGC CTGCTCATGAGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGA GTTTATCTCCTGATGAAGAAGCTAGCCTTCTGA >OriGene 5' read for NM_006232 unedited
GGTTCACATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCGC TTTCAGGAGGTGCTTTTGGTTCTCTCCGGTCTTGTCCACGCTAGGGGGTGCACGTACTCC CAACTGTGGTCGCGCTCTCACCCCTTCTGCTGCTCTCGTGGCCCCCTCGCGATGGCGGGC ATCCTGTTTGAGGATATTTTCGATGTGAAGGATATTGACCCGGAGGGCAAGAAGTTTGAC CGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATCTTAGATGTA AACATTCAAATTTACCCTGTAGACTTGGGTGACAAGTTTCGGTTGGTCATAGCTAGTACC TTGTATGAAGATGGTACCCTGGATGATGGTGAATACAACCCCACTGATGATAGGCCTTCC AGGGCTGACCAGTTTGAGTATGTAATGTATGGAAAAGTGTACAGGATTGAGGGAGATGAA ACTTCTACTGAAGCAGCAACACGCCTCTCTGCGTACGTGTCCTATGGGGGCCTGCTCATG AGGCTGCAGGGGGATGCCAACAACCTGCATGGATTCGAGGTGGACTCCAGAGTTTATCTC CTGATGAAGAAGCTAGCCTTCTGAACCTCGCCTGAAGCCAGCCTCTCTGCCAAGTCACTC AGGTCATGGGCATTGTTCAAGCCTGAGTGGCAGCCGCTCTTGCTCACCTGTTGAGGAAGG GCTGGCTCACTGTCCACCGTGGCGGCATCTTTAACTGGCCTCCACTCAATGGGAAACTGA CTCGCCTGTGAAAGACACAGTNGGAGAGCTGCAAATGAATCAGAAGCTNTATGTATATGA TTTTTAATTAAACTTTACTTTTTCAGAANAAAAACAAAAAAACTCGACTCTAGATTGCGG CCGCGGTCATAN >OriGene 3' read for NM_006232 unedited
NAGCTTGTACCCGGCCGCAATCNANGATCGGTTTTTTTTTTTTTTTTTTCTGAAAAGTAA AGTTTAATTTAAAAATCATATACATAAAGCTTCTGATTCATTTTCAGCTCTCCCACTGTG TCTTTCACAGGCGAGTCAGTTTCCCATTGAGTGGAGGCCAGTTAAAGATGCCGCCACGGT GGACAGTGAGCCAGCCCTTCCTCAACAGGTGAGCATGATCGGCTGCCACTCAGGCTTGAA CAATGCCCATGACCTGAGTGACTTGGCAGAGAGGCTGGCTTCAGGCGAGGTTCAGAAGGC TAGCTTCTTCATCAGGAGATAAACTCTGGAGTCCACCTCGAATCCATGCAGGTTGTTGGC ATCCCCCTGCAGCCTCATGAGCAGGCCCCCATAGGACACGTACGCAGAGAGGCGTGTTGC TGCTTCAGTAGAAGTTTCATCTCCCTCAATCCTGTACACTTTTCCATACATTACATACTC AAACTGGTCAGCCCTGGAAGGCCTATCATCAGTGGGGTTGTATTCACCATCATCCAGGGT TCCATCTTCCTACAAGGTACTAGCTATGACCAACCGAAACTTGTCCCCCAAGTCTACAGG GTAAATTTGAATGTTTACCTCCTAGATTAGACCCATCTTGAAAGATTCACTCTTACCTTG CAGCCCAAACCCTTCGTCAACCTTTGGCCCTCCGGGCCATTTCCCTCCCTTGGAAATTTC CCCAAACGGCTGCCCGGCCTCTCGAGGGGGCCCCCTTACCCCCTCAGGGGTGAGACGCCG CCCCCGCTGGGGAATCCCCCCCCCCTTCCGTGGTCCAACTGGTGGGACCCTTCCCCCCTC TGTAACCCCCCCTTTGGTCGCTTTCCCGGCCGTTTTTTCGGGTCCCGTTCCATATTTTGT GGTCCCCTTTTGTGTCCTTGTGTTTCTATCATACTTACGCCACAACAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_006232 |
Insert Size | 840 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006232.2, NP_006223.2 |
RefSeq Size | 821 bp |
RefSeq ORF | 453 bp |
Locus ID | 5437 |
UniProt ID | P52434 |
Domains | RNA_pol_Rpb8 |
Protein Families | Transcription Factors |
Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
Gene Summary | The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200650 | POLR2H (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H) |
CNY 1,200.00 |
|
RC200650L3 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200650L4 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H), mGFP tagged |
CNY 5,890.00 |
|
RG200650 | POLR2H (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide H (POLR2H) |
CNY 2,800.00 |