United States
Germany
Japan
United Kingdom
China
CNOT10 (NM_015442) Human Untagged Clone
CAT＃: SC112646
CNOT10 (untagged)-Human CCR4-NOT transcription complex, subunit 10 (CNOT10)
CNY 8,184.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112646 sequence for NM_015442 edited (data generated by NextGen Sequencing)
ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAACATGAAGGCACAGGTCAGTCC TCTGGGATCACTGATCAAGAGAAGGAGTTATCCACCAATGCTTTCCAAGCTTTCACATCT GGAAATTATGATGCCTGTCTACAACACCTTGCCTGTCTACAAGATATAAACAAAGATGAT TATAAAATAATTTTGAATACAGCAGTAGCTGAGTTTTTTAAAAGTAACCAAACAACAACA GATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGGTCCACTCAGCTGTTGAAGAA ATGGATGGATTAGATGATGTTGAAAACAGCATGTTGTACTATAATCAAGCAGTCATTCTT TATCATCTGCGGCAGTATACAGAAGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATA GAGCCTTTTNNNNNNAAATTTGCCCAAGCAGTGTGTTTTTTGCTTGTAGACCTGTATATA TTAACCTACCAAGCTGAGAAAGCTTTGCATCTTCTTGCTGTCCTAGAAAAAATGATTTCA CAGGGTAACAATAACAAAAATGGAAAGAATGAGACTGGTAATAACAACAACAAAGATGGA TCTAATCATAAAGCTGAAAGTGGAGCTCTAATAGAAGCTGCAAAATCAAAGATACATCAG TACAAAGTACGAGCTTATATCCAAATGAAGTCTCTGAAAGCATGTAAAAGGGAAATCAAG TCAGTCATGAATACAGCTGGAAATTCCGCACCCTCTCTCTTTCTTAAAAGCAATTTTGAG TACTTAAGAGGTAATTATCGAAAAGCCGTGAAGCTATTAAATAGTTCAAACATTGCTGAG CATCCAGGATTCATGAAAACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGT TGCATCCATTTTGCCATGAGCAAGCACAATTTGGGAATATTCTACTTTAAAAAGGCTCTG CAAGAGAATGACAATGTCTGTGCACAGCTCAGTGCAGGTAGCACTGATCCAGGTAAAAAA TTTTCAGGAAGACCCATGTGTACGTTACTAACCAATAAGAGATATGAGTTGCTGTATAAC TGTGGAATTCAGCTTCTTCACATTGGAAGGCCTCTTGCTGCCTTCGAATGTCTGATTGAA GCTGTTCAGGTTTATCATGCAAATCCTCGCCTCTGGCTACGGCTGGCTGAATGCTGCATT GCTGCCAATAAGGGGACTTCTGAACAAGAAACTAAAGGCCTTCCCAGCAAAAAAGGAATT GTACAGTCTATTGTTGGTCAAGGCTATCATCGTAAAATAGTTTTGGCATCACAGTCTATA CAGAATACTGTTTATAATGATGGGCAGTCTTCGGCCATTCCTGTAGCCAGTATGGAGTTT GCAGCCATATGTCTCAGAAATGCCTTGTTGCTGCTACCTGAAGAACAGCAAGATCCAAAG CAGGAAAATGGGGCTAAAAATAGTAATCAATTAGGTGGGAACACAGAGAGCAGCGAAAGC AGTGAAACTTGCAGCAGTAAAAGCCATGATGGAGATAAATTCATTCCAGCTCCACCTTCT TCTCCATTGAGAAAACAGGAATTAGAAAACTTAAAGTGCTCCATACTTGCTTGCAGTGCC TACGTGGCTCTGGCTTTGGGTGATAACCTCATGGCTTTGAATCATGCAGATAAACTTCTT CAGCAGCCCAAGCTGTCAGGATCTCTTAAGTTTTTGGGACATTTATATGCTGCAGAAGCC CTCATCTCTCTCGACAGAATATCTGATGCCATTACTCACTTGAACCCGGAGAATGTCACT GATGTCTCCTTAGGGATCTCTTCAAATGAGCAGGACCAAGGATCAGACAAAGGTGAAAAT GAAGCAATGGAATCCTCTGGTAAGCGGGCCCCTCAGTGCTACCCCAGTTCCGTCAACTCT GCCAGGACTGTGATGCTGTTCAACCTTGGCAGCGCTTACTGCCTGAGGAGCGAATATGAC AAAGCCCGAAAGTGTCTCCACCAGGCGGCTTCAATGATCCATCCTAAAGAGGTGCCCCCT GAGGCCATCTTGCTGGCAGTCTACCTTGAACTGCAGAATGGTAATACTCAGCTGGCCTTA CAGATCATCAAAAGGAATCAGCTGCTCCCTGCAGTGAAAACACACTCTGAAGTGAGAAAG ANNNNNNNNNTTCAGCCTGTCCACCCGATCCAGCCCATCCAAATGCCGGCTTTCACCACT GTGCAGAGAAAGTGA Clone variation with respect to NM_015442.1 430 g=>n;431 a=>n;432 a=>n;433 g=>n;434 a=>n;435 a=>n;1014 c=>t;2162 a=>n;2163 g=>n;2164 c=>n;2165 c=>n;2166 a=>n;2167 g=>n;2168 t=>n;2169 g=>n;2170 t=>n >OriGene 5' read for NM_015442 unedited
CGGCACATTTGTATACGATCACTATAGGCGGCCGCGCAATTCGCACGAGCCTCGTGCCGA ATTCGGCACGAGGCGGAGGTCCAGGACAGCGGCCAGCCCGGCGGCGGGAGTCAGGGCCAC GCCACCTGCAGGGAAGAACCCGAGTCGAAGCGGGAAGATGGCTGCAGACAAGCCTGCAGA TCAGGGAGCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATCACTGATCAAGAGAA GGAGTTATCCACCAATGCTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGTCTACA ACACCTTGCCTGTCTACAAGATATAAACAAAGATGATTATAAAATAATTTTGAATACAGC AGTAGCTGAGTTTTTTAAAAGTAACCAAACAACAACAGATAATTTGAGACAAACACTTAA CCAGCTGAAGAATCAGGTCCACTCAGCTGTTGAAGAAATGGATGGATTAGATGATGTTGA AAACAGCATGTTGTACTATAATCAAGCAGTCATTCTTTATCATCTGCGGCAGTATACAGA AGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATAGAGCCTTTTGAAAAATTTGCCCA AGCAGTGTGTTTTTTGCTTGTAGACCTGTATATATTAACCTACCAAGCTGAGAAAGCTTT GCATCATCTTGCTGTCCTAGCAAAAATGATTTCACAGGGTAACAATAACAAAAATGGAAA GAAGGAAACTGGTCATAACCCCACCACAGAGGATCTTAACTTAAAGCTGAAAGGGGAGCT CTAATAAAGCTGTCAAATCAAGATACTTCGTTCAAGGTACGAGCTTATTTCCCAAAGAAC GTCTTTGAAGCCTGTTAAAGGGGAAACAAGTCACTCTTGAAAACTGCTGGGAAATCTCCC CATCTCCTCTTTTTTTAAAGGCCCATTTGGGC >OriGene 3' read for NM_015442 unedited
CGGCCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCTGATAAAATTATGTTTTAA TTGCCTATGGCATATATTACATAATGGCTCAAAATTAATAGTCTGGCTGAAATTAAGAAA AACAGCTGATGATGACTTAGTAAATCCTTAATTTTAAGTAAGCTTTTGGCCAAAAGTCAG GGGTAGAATTGACTCAAAATTAACAGCCTTCACCATCTTTTATTCATTCTGCTGTGATAC AACTAAAATGGCCAGTAAATTCTCCCCTGGGTCTCAGGTAACAGTTTTCCAAAAGTGAAG TATCACTTTCTCTGCACAGTGGTGAAAGCCGGCATTTGGATGGGCTGGATCGGGTGGACA GGCTGAAACACTGGCTTCTTTCTCACTTCAGAGTGTGTTTTCACTGCAGGGAGCAGCTGA TTCCTTTTGATGATCTGTAAGGCCAGCTGAGTATTACCATTCTGCAGTTCAAGGTAGACT GCCAGCAAGATGGCCTCAGGGGGCACCTCTTTAGGATGGATCATTGAAGCCGCCTGGTGG AGACACTTTCGGGCTTTGTCATATTCGCTCCTCAGGCAGTAAGCGCTGCCAAGGTTGAAC AGCATCACAGTCCTGGCAGAGTTGACGGAACTGGGGTAGCACTGAGGGGCCCGCTTACCA GAGGATTCCATTGCTTCATTTTCACCTTTGTCTGATCCTTGGTCCTGCTCATTTGAAGAG ATCCCTAGGGAACATCAGTGACATTCTCCGGGTTCAAGTGAGTAATGGCATCANATATTC TGTCGAGAGGATGAGGGCTTCTGCAGCATATAAATGTCCCAAAAACTAANAGATCTGACA GCTTGGGCTGCTGGANAAGTTTATCTGCATGATTCAAAGCCTGAGGGTATCACCCAANCC AAAGCCCGTAGGCCTGCAGGCAGTTGGAGCCCTTTTAGTTTCTAATCCCTGTTCTTAATG GAAAAAAGGGAGCTGGAAGATTATCTCCTCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015442 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015442.1, NP_056257.1 |
| RefSeq Size | 2741 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 25904 |
| UniProt ID | Q9H9A5 |
| Domains | TPR |
| Protein Pathways | RNA degradation |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202651 | CNOT10 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 10 (CNOT10) |
CNY 8,176.00 |
|
| RC202651L3 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 10 (CNOT10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202651L4 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 10 (CNOT10), mGFP tagged |
CNY 5,890.00 |
|
| RG202651 | CNOT10 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 10 (CNOT10) |
CNY 4,370.00 |
