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CDC25B (NM_021872) Human Untagged Clone
CAT＃: SC112556
CDC25B (untagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3
CNY 8,650.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_021872 edited
GAATTCGGCACGAGGCTCTCCTCCCTCGCGCCTGGCCCTGTGGCTCTTCCTCCCTCCCTC CTTCCCCCCCCCCCCACCCCTCGCCCGCTGCCTCCCTCGGCCCAGCCAGCTGTGCCGGCG TTTGTTGGCTGCCCTGCGCCCGGCCCTCCAGCCAGCCTTCTGCCGGCCCCGCCGCGATGG AGGTGCCCCAGCCGGAGCCCGCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTG GCGCCCAGCGTCCGGGCCACCTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGT CCCCGGTGCGGGCGGCCGCTTCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACC TCGCCGGGCTCGGCAGCGAAACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCC GCAGCCGCCTGACGCACCTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTG AATCCTCCGAATCTTCTGATGCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCC ACATGGCGGAGCAGACGTTTGAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACG AGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTGAGGCTGCTGGGCCACAGCCCCG TGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAGGCGGGCA GTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGC CATGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGG AAGCCTTTGCCCAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGA AGATGGAAGTGGAGGAGCTCAGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAG AGGGGGATACTGAGGAAGATGATGGATTTGTGGACATCCTAGAGAGTGACTTAAAGGATG ATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGTGCCCCACTGGTCAAGACCTTGG AAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGGCTCTTCCGCTCTC CGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAGGACA GGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGG AGGCTGAGGAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCG AGAACCTCCTGGACAGTGACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCC TACAGACAGTAGACGGAAAGCACCAAGACCTCAAGTACATCTCACCAGAAACGATGGTGG CCCTATTGACGGGCAAGTTCAGCAACATCGTGGATAAGTTTGTGATTGTAGACTGCAGAT ACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTGAACTTGCCCCTGGAACGCG ACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGACAAGAGAGTCA TCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATCA GGGAACGAGACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCC TGAAAGGCGGCTACAAGGAGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACT ACCGGCCCATGAACCACGAGGCCTTCAAGGATGAGCTAAAGACCTTCCGCCTCAAGACTC GCAGCTGGGCTGGGGAGCGGAGCCGGCGGGAGCTCTGTAGCCGGCTGCAGGACCAGTGAG GGGCCTGCGCCAGTCCTGCTACCTCCCTTGCCTTTCGAGGCCTGAAGCCAGCTGCCCTAT GGGCCTGCCGGGCTGAGGGCCTGCTGGAGGCCTCAGGTGCTGTCCATGGGAAAGATGGTG TGGGTGTCCTGCCTGTCTGCCCCAGCCCAGATTCCCCTGTGTCATCCCATCATTTTCCAT ATCCTGGTGCCCCCCACCCCTGGAAGAGCCCAGTCTGTTGAGTTAGTTAAGTTGGGTTAA TACCAGCTTAAAGGCAGTATTTTGTGTCCTCCAGGAGCTTCTTGTTTCCTTGTTAGGGTT AACCCTTCATCTTCCTGTGTCCTGAAACGCTCCTTTGTGTGTGTGTCAGCTGAGGCTGGG GGAGAGCCGTGGTCCCTGAGGATGGGTCAGAGCTAAACTCCTTCCTGGCCTGAGAGTCAG CTCTCTGCCCTGTGTACTTCCCGGGCCAGGGCTGCCCCTAATCTCTGTAGGAACCGTGGT ATGTCTGCCATGTTGCCCCTTTCTCTTTTCCCCTTTCCTGTCCCACCATACGAGCACCTC CAGCCTGAACAGAAGCTCTTACTCTTTCCTATTTCAGTGTTACCTGTGTGCTTGGTCTGT TTGACTTTACGCCCATCTCAGGACACTTCCGTAGACTGTTTAGGTTCCCCTGTCAAATAT CAGTTACCCACTCGGTCCCAGTTTTGTTGCCCCAGAAAGGGATGTTATTATCCTTGGGGG CTCCCAGGGCAAGGGTTAAGGCCTGAATCATGAGCCTGCTGGAAGCCCAGCCCCTACTGC TGTGAACCCTGGGGCCTGACTGCTCAGAACTTGCTGCTGTCTTGTTGCGGATGGATGGAA GGTTGGATGGATGGGTGGATGGCCGTGGATGGCCGTGGATGCGCAGTGCCTTGCATACCC AAACCAGGTGGGAGCGTTTTGTTGAGCATGACAGCCTGCAGCAGGAATATATGTGTGCCT ATTTGTGTGGACAAAAATATTTACACTTAGGGTTTGGAGCTATTCAAGAGGAAATGTCAC AGAAGCAGCTAAACCAAGGACTGAGCACCCTCTGGATTCTGAATCTCAAGATGGGGGCAG GGCTGTGCTTGAAGGCCCTGCTGAGTCATCTGTTAGGGCCTTGGTTCAATAAAGCACTGA GCAAGTTGAGAAACCAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_021872 unedited
GGTTCAAATTTTGNTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCTC CTCCCTCGCGCCTGGCCCTGTGGCTCTTCCTCCCTCCCTCCTTCCCCCCCCCCCCACCCC TCGCCCGCTGCCTCCCTCGGCCCAGCCAGCTGTGCCGGCGTTTGTTGGCTGCCCTGCGCC CGGCCCTCCAGCCAGCCTTCTGCCGGCCCCGCCGCGATGGAGGTGCCCCAGCCGGAGCCC GCGCCAGGCTCGGCTCTCAGTCCAGCAGGCGTGTGCGGTGGCGCCCAGCGTCCGGGCCAC CTCCCGGGCCTCCTGCTGGGATCTCATGGCCTCCTGGGGTCCCCGGTGCGGGCGGCCGCT TCCTCGCCGGTCACCACCCTCACCCAGACCATGCACGACCTCGCCGGGCTCGGCAGCGAA ACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCCGCAGCCGCCTGACGCACCTA TCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTGAT GCAGGTCTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTT GAACAGGCCATCCAGGCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGC TTCCAGTCTATGCCGGTGAGGCTGCTGTGCCACAGCCCCGTGCTTCGGACATCACCAACT CCCAGCGCCCGACGGCCGGAGGAGAGCGAAGCGGGCAGTGTAGCTGCCAACAGCTCTGGG GGAAGACAAGAAGATGATGGATTTGGTCTTCAAGAGCCATGGNAGNCCACACATTCCACT CCACCCATGCTCTGCCAAAGTGGCCACCCGCAGGGAGCCTTTGCCCAGAGACCAGCTCGC CCCCGACCTGATGTGTCTAGTCC >OriGene 3' read for NM_021872 unedited
CGCTTTTAGTATCGAGTTTTTTTTTTTTTTTTTTGGTTCTCTCAACTTGTCTCAGTGGCT TTATTTGAACCAAGGGCCCTAACAGATGACTCAGCAGGGCCTTCAAGCACAGCCCTGCCC CCATCTTGAGATTCAGAATCCAGAGGGTGCTCAGTCCTTGGTTTAACTGCTTCTGTGACA TTTCCTCTTGAATAGCTCCAAACCCTAAGTGTAAATATTTTTGTCCACACAAATAGGCAC ACATATATTCCTGCTGCAGGCTGTCATGCTCAACAAAACGCTCCCACCTGGTTTGGGTAT GCAAGGCACTGCGCATCCACGGGCATCCACGGGCATCCACCCATCCATCCAACCTTCCAT CCATCCGCAACAAGACAGCAGCAAGTTCTGAGCAGTCAGGCCCCAGGGTTCACAGCAGTA GGGGCTGGGCTTCCAGCAAGCTCATGATTCAAGCCTTAACCCTTGCCCTGGGAGCCCCCA AGGATAATAACATCCCTTTCTGGGGCAACAAAACTGGGACCGAGTGGGTAACTGATATTT GACAGGGGAACCTAAACAGTCTACGGAAGTGTCCTGAGATGGGCGTAAAGTCAAACAGAC CAAGCACACAGGTAACACTGAAATANGAAAGAGTAAGAGCTTCTGTTCAAGCTGGAGGTG CTCGTATGGTGGGACAGGAAAGGGGAATAGAGAAGGGGGCACATGGCAGACATACACCGT TCCTACAAAGATAGGGGCAGCCCTGGCCCGGAAGTCACAGGGCAGGAGCTGACCTCAGCC AGAATGAGTTAGCTCTGACCATCCTAAGGACACCGGTTTCCCCAGCTCACTGAACAACAC AAGGACGTTTAGACCAGAAAATAAGGTTAACCTACAGGAACAGAGCTCTGAGAACAATCC GCCTTAACTGGTTACCCACTATCACTAAAAACTGCTTTCAGGGTGGGGCCCGTATTGAAT GTGGTGACAGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021872 |
| Insert Size | 3120 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021872.2, NP_068658.1 |
| RefSeq Size | 3578 bp |
| RefSeq ORF | 1620 bp |
| Locus ID | 994 |
| UniProt ID | P30305 |
| Domains | RHOD |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation |
| Gene Summary | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3), also known as CDC25B2, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3) compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
F-box protein 7 mutations promote protein aggregation in mitochondria and inhibit mitophagy.
,null,
Human molecular genetics
,PubMed ID 26310625
[CDC25B]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215766 | CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 |
CNY 4,320.00 |
|
| RC215766L3 | Lenti-ORF clone of CDC25B (Myc-DDK-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 |
CNY 6,650.00 |
|
| RC215766L4 | Lenti-ORF clone of CDC25B (mGFP-tagged)-Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 |
CNY 6,650.00 |
|
| RG215766 | CDC25B (tGFP-tagged) - Human cell division cycle 25 homolog B (S. pombe) (CDC25B), transcript variant 3 |
CNY 5,920.00 |
