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KLHL25 (NM_022480) Human Untagged Clone
CAT＃: SC112494
KLHL25 (untagged)-Human kelch-like 25 (Drosophila) (KLHL25)
CN¥ 4,392.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ENC-2; ENC2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112494 sequence for NM_022480 edited (data generated by NextGen Sequencing)
ATGTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTC ACCCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGC AAGCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCAC CGTGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGG GAGAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTG CTGCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTG CTGGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTG GAGAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAG TGCCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGG CAGAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGAT GAGCTGGAGACCGAGGACGAGCGGGTGGTCTTCGAGGCCATCCTCCAGTGGGTGAAGCAC GACCTGGAGCCACGGAAGGTCCACTTGCCCGAGCTCCTCCGCAGCGTGCGTCTGGCCTTG CTGCCGTCCGACTGCCTGCAGGAGGCCGTCTCCAGCGAGGCCCTCCTCATGGCAGACGAG CGCACCAAGCTTATCATGGATGAGGCCCTGCGCTGCAAGACCAGGATCCTGCAGAATGAT GGCGTGGTCACCAGCCCCTGTGCCTGGCCACGCAAGGCGGGCCACACGCTACTCATCCTG GGGGGCCAGACCTTCATGTGTGACAAGATCTACCAGGTGGACCACAAGGCCAAGGAGATC ATCCCCAAGGCCGACCTGCCCAGCCCCCGGAAGGAGTTCAGCGCCTCAGCGATCGGCTGC AAGGTCTATGTGACGGGGGGCAGGGGCTCCGAGAACGGGGTCTCCAAGGATGTCTGGGTG TACGACACCGTACATGAGGAATGGTCCAAGGCGGCGCCCATGCTGATTGCCCGCTTTGGC CATGGCTCAGCTGAGCTGGAGAACTGCCTCTATGTGGTGGGGGGACACACATCCCTGGCA GGGGTCTTCCCGGCCTCGCCTTCTGTCTCCCTGAAACAAGTGGAGAAATACGACCCTGGG GCCAACAAGTGGATGATGGTGGCCCCCTTGCGGGATGGCGTCAGCAATGCCGCAGTGGTG AGTGCCAAGCTGAAGCTCTTTGTTTTCGGAGGAACCAGCATCCACCGGGACATGGTGTCC AAGGTCCAGTGCTATGACCCCTCGGAGAACAGGTGGACGATCAAGGCCGAGTGCCCCCAG CCTTGGCGGTACACAGCCGCTGCCGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGAC ACGGAATTCACAGCCGCCTCGGCCTACCGCTTTGACTGTGAGACCAACCAGTGGACGCGG ATTGGGGACATGACTGCCAAGCGCATGTCCTGCCATGCCCTGGCTTCCGGCAACAAGCTC TATGTGGTCGGGGGCTACTTTGGGACCCAGAGGTGTAAGACTCTGGACTGCTATGACCCC ACTTCAGATACATGGAACTGCATCACCACAGTGCCCTACTCACTTATCCCCACGGCCTTT GTCAGCACCTGGAAGCACCTGCCCGCGTGA Clone variation with respect to NM_022480.3 865 c=>t >OriGene 5' read for NM_022480 unedited
GCGAATTTTGTNATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGCGTTTTT GCCTGTGGAGAGCCGGCCGACGGGAGCCGCGGCGGAGCCTGTTGAGCTCGCGCGGGCTGC CGGGAGTGGTCTCTGAGGCGGCGGCGGCGGCGGGGATCGTCTCCGGCACTGGCGCACCAT GTCGGTCAGTGTCCATGAGACCCGCAAGTCGCGGAGCAGCACGGGGTCCATGAACGTCAC CCTCTTCCACAAGGCCTCCCACCCGGACTGTGTGCTGGCCCACCTCAACACGCTTCGCAA GCACTGCATGTTCACCGACGTCACACTCTGGGCGGGCGACCGTGCCTTCCCCTGTCACCG TGCCGTGCTGGCCGCCTCTAGCCGCTATTTTGAGGCCATGTTCAGCCATGGCCTTCGGGA GAGCCGGGATGACACTGTCAACTTCCAGGACAACCTGCACCCGGAGGTGCTGGAGCTGCT GCTGGACTTTGCCTACTCCTCACGCATCGCCATCAACGAGGAGAACGCTGAGTCACTGCT GGAGGCAGGCGACATGCTGCAGTTCCACGATGTGCGGGATGCTGCCGCCGAGTTCCTGGA GAAGAACCTTTTCCCCTCCAACTGCCTGGGCATGATGCTGCTCTCGGACGCCCACCAGTG CCGCCGGCTGTATGAGTTCTCCTGGCGCATGTGCCTGGTGCACTTTGAGACGGTGAGGCA GAGCGAGGACTTCAACAGCCTGTCCAAGGACACACTGCTGGACCTCATCTCGAGTGATGA GCTGGAGACCGAGACGAGCGGGTGGTCTTCGAGGCCTCCTCCAGTGGGTGAAGCCGACCT GGAGCCACCGGAGGTCCACTGCCCGAGCTCTCCGCAGCGTGCGTCTGGCCTTGCTGCCGT NCGACTGCCCTGCAGGAGGGCGTCTCCAGCGAGGCCCTCCTCATGGCA >OriGene 3' read for NM_022480 unedited
NTAAGCTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTCTC ACACTCTTTTTATTAAGTTAGAAAACTGATAAAAGCAACACAACTTTTGGGGAAAGCACC ATGGCACGTCCTTTGTGCTATGTGATAAGCGTGCTTTATCTCAATGAAGCAACCCCACGG GTCCAGGCACCCTCCCTGCAGTCCCCGCGGCCAGGCTCCTGAGTGTGCCAGCTGAGCCGT CCCCTGGGACCACAGCCAAGTGCCTTCGGGCAGCTGGCCTGACACAGGCGGGGTCTGCTG GGTCTACAGGGTCCAAGGAGCCCCATGCAGCCAGTGCCCCATGGCGGGCGCGTCAGTGGG CAAACCCTGCCCAAAGGTCCCACCCCCAAGAGGCCTCCAGGACCCGCACCAAGGCATGGG GGACACTCGCGGCTGCTTAAGTAACTGGTATGTGCACAGCCCCCTCCGCGGTCCTAACCT TGAGTCAGGCCAGCAGTGGCTCCACTGCAGGCGCCTGGGTAGTCCCACCCTTGCACCCCC TGATCACCTTCTGTCAAGATGCCACCCCCCCTCCCTTCCTCACGGCTGTCTCGACCTCTA ATTTACAGGCTCTATCCCGCTTTTTCGTTTGCCGCATGCCTTCCCTCCCAGCCGTGAAGC TGCTGTGTCTAGATCCTCCCCATTNATTTCTTTTCTCCTCTCTTTTTGGTTTGCTTCATT CTGGTTTGCTATATGCCGCACCTCGGCATCACCGTCGGTCGTCCTCCCGCGCGCTCTCAC GCCTGGCTTTACTTATTATCGTCTTCCGTCATCCTTTCGCTNGACTCTGTCTAGTCTTCC TATCTCACTCCCGCTCCACGCGCCNCTCCACCATACGCCCGCTCCCTTACNTCCGCAGCT CATCAGCCCGTGCTCCTCCATGACGCCACACCTACTCCCCTCTCGCACCGCCGCCGTGAC GTACCTGCGCATTGTCCGCCCACATCCACACGCCCACCTCCCNCCTGNTCTTACATATGT NTATCAGCGTACGGCGAGTCGCGGCACACCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022480 |
| Insert Size | 3600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022480.2, NP_071925.2 |
| RefSeq Size | 3525 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 64410 |
| UniProt ID | Q9H0H3 |
| Domains | BTB, Kelch |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206979 | KLHL25 (Myc-DDK-tagged)-Human kelch-like 25 (Drosophila) (KLHL25) |
CN¥ 4,384.00 |
|
| RC206979L1 | Lenti ORF clone of Human kelch-like 25 (Drosophila) (KLHL25), Myc-DDK-tagged |
CN¥ 6,784.00 |
|
| RC206979L2 | Lenti ORF clone of Human kelch-like 25 (Drosophila) (KLHL25), mGFP tagged |
CN¥ 5,890.00 |
|
| RC206979L3 | Lenti ORF clone of Human kelch-like 25 (Drosophila) (KLHL25), Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC206979L4 | Lenti ORF clone of Human kelch-like 25 (Drosophila) (KLHL25), mGFP tagged |
CN¥ 5,890.00 |
|
| RG206979 | KLHL25 (tGFP-tagged) - Human kelch-like 25 (Drosophila) (KLHL25) |
CN¥ 5,984.00 |
