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SMURF 2 (SMURF2) (NM_022739) Human Untagged Clone
CAT＃: SC112475
SMURF2 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2)
CNY 5,488.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_022739 edited
GAATTCGGCACGAGGGGAAGCGGCCGAGCCCAGAGTTCTGCTCCGGCGCCCCCGAGCACC GCCCGCTTCAGCCGACCAGCCCCGTCGGCTACTGGGCCTCGCCGAGACGAGAGGAGGGAA AGGCCTCGGCGGCCGCCGCCCCCGCCGCCGGGGACATGTCTAACCCCGGAGGCCGGAGGA ACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACCTGGTGAAAAAGGATT TTTTCCGACTTCCTGATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAATGCCATT CTACAGATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGACCTGTATA TTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGAAGATCCATAAGAAAC AAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGCCTCAAAG ACACTGGTTATCAGAGGTTGGATTTATGCAAACTCGGGCCAAATGACAATGATACAGTTA GAGGACAGATAGTAGTAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGACAAGTTG TGGACTGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGGAGAACCG CCTCTGGAAGAATCCAGTATCTAAACCATATAACAAGAACTACGCAATGGGAGCGCCCAA CACGACCGGCATCCGAATATTCTAGCCCTGGCAGACCTCTTAGCTGCTTTGTTGATGAGA ACACTCCAATTAGTGGAACAAATGGTGCAACATGTGGACAGTCTTCAGATCCCAGGCTGG CAGAGAGGAGAGTCAGGTCACAACGACATAGAAATTACATGAGCAGAACACATTTACATA CTCCTCCAGACCTACCAGAAGGCTATGAACAGAGGACAACGCAACAAGGCCAGGTGTATT TCTTACATACACAGACTGGTGTGAGCACATGGCATGATCCAAGAGTGCCCAGGGATCTTA GCAACATCAATTGTGAAGAGCTTGGTCCATTGCCTCCTGGATGGGAGATCCGTAATACGG CAACAGGCAGAGTTTATTTCGTTGACCATAACAACAGAACAACACAATTTACAGATCCTC GGCTGTCTGCTAACTTGCATTTAGTTTTAAATCGGCAGAACCAATTGAAAGACCAACAGC AACAGCAAGTGGTATCGTTATGTCCTGATGACACAGAATGCCTGACAGTCCCAAGGTACA AGCGAGACCTGGTTCAGAAACTAAAAATTTTGCGGCAAGAACTTTCCCAACAACAGCCTC AGGCAGGTCATTGCCGCATTGAGGTTTCCAGGGAAGAGATTTTTGAGGAATCATATCGAC AGGTCATGAAAATGAGACCAAAAGATCTCTGGAAGCGATTAATGATAAAATTTCGTGGAG AAGAAGGCCTTGACTATGGAGGCGTTGCCAGGGAATGGTTGTATCTCTTGTCACATGAAA TGTTGAATCCATACTATGGCCTCTTCCAGTATTCAAGAGATGATATTTATACATTGCAGA TCAATCCTGATTCTGCAGTTAATCCGGAACATTTATCCTATTTCCACTTTGTTGGACGAA TAATGGGAATGGCTGTGTTTCATGGACATTATATTGATGGTGGTTTCACATTGCCTTTTT ATAAGCAATTGCTTGGGAAGTCAATTACCTTGGATGACATGGAGTTAGTAGATCCGGATC TTCACAACAGTTTAGTGTGGATACTTGAGAATGATATTACAGGTGTTTTGGACCATACCT TCTGTGTTGAACATAATGCATATGGTGAAATTATTCAGCATGAACTTAAACCAAATGGCA AAAGTATCCCTGTTAATGAAGAAAATAAAAAAGAATATGTCAGGCTCTATGTGAACTGGA GATTTTTACGAGGCATTGAGGCTCAATTCTTGGCTCTGCAGAAAGGATTTAATGAAGTAA TTCCACAACATCTGCTGAAGACATTTGATGAGAAGGAGTTAGAGCTCATTATTTGTGGAC TTGGAAAGATAGATGTTAATGACTGGAAGGTAAACACCCGGTTAAAACACTGTACACCAG ACAGCAACATTGTCAAATGGTTCTGGAAAGCTGTGGAGTTTTTTGATGAAGAGCGACGAG CAAGATTGCTTCAGTTTGTGACAGGATCCTCTCGAGTGCCTCTGCAGGGCTTCAAAGCAT TGCAAGGTGCTGCAGGCCCGAGACTCTTTACCATACACCAGATTGATGCCTGCACTAACA ACCTGCCGAAAGCCCACACTTGCTTCAATCGAATAGACATTCCACCCTATGAAAGCTATG AAAAGCTATATGAAAAGCTGCTAACAGCCATTGAAGAAACATGTGGATTTGCTGTGGAAT GACAAGCTTCAAGGATTTACCCAGGACTCTATTTATACAACCCTGACTGACAGCCTCCTT TCAGCAGAGTTTCAAAGAATATGCTGAAATACAGGAAAACACTCCCCCCCCCCCTTTTTT TTTTTTTTTTTTACATTTTAGGACACTGTGAGGGGAAAGGACAATTTTGAAATTCCTTTT CAAGGAAAAAAAAGGTCTTTATGCTTTGCCATGAGGCCACATTCAGCTGCTATTTAAACT TAATATCTTGAACCTAAAGAATGCTGACTTTTCCTACATTTCCAGAGTTAGGCAGTATTC TACACTTAAAGACTACTACTATTTTTATAAAAGGTAATCTATTCAAATTTCTTCACAGAT TTCAAGTCTCTCAAACATCAAGACAACTTCAGCAGTCGGTACAAGTCACATTTCATTTTG ATTGAATACATGATCTTGAACAGCTCCTGTACTTGCTCTTTGTAAAAAAAAATAAAATTA TTTTGAATTATTCTACCTTTGTAAACAATTGGCTAAAAGAATCATCTTTAAGAAATTAAG CCATTTACATGTTTGTGTTTTTCTATAGCAGAGCATTATATTTTGCATTATATGTTTCAA CCTAGTCTAAGTGGGTCTTTTTTACATTTTTCAAGAACGGATTTCCTGGAATACAGCGAT ATAATTTTGGTTGTCAAATTCCTAATGCAACCATTTAGTCTAAACTTAGTCATTTATTTG TGACAATAAGATGTGTTCAGGGGCTCCCTGTTTTTAAGAGACTCTTTTAAAAAAAAAAAA ACCTAATGTTTTTATCTTGAGTCAATATGATTAGGTATTTTGGATTTACTTTTAATCTTA AAATACTGCATTTTTATAGCTTCTCAGAGCATGTGGATGGGATGGGATTTTCGTTATTTT GCTGGGTCAGCTTATCTXXXXXXXXXXXXXXXXXXXXXXXXXGAGATTGCAGTGAGCCGA GATCGCACCACTGCACTCCAGCCTGGGCGACAAGAGTGAGACTCCGTCCCCGCCCAAAAA AAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_022739 unedited
NGGCTTCTTACCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAAGGTCT ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG CGGCCGCGAATTCGGCACGAGGGGAAGCGGCCGAGCCCAGAGTTCTGCTCCGGCGCCCCC GAGCACCGCCCGCTTCAGCCGACCAGCCCCGTCGGCTACTGGGCCTCGCCGAGACGAGAG GAGGGAAAGGCCTCGGCGGCCGCCGCCCCCGCCGCCGGGGACATGTCTAACCCCGGAGGC CGGAGGAACGGGCCCGTCAAGCTGCGCCTGACAGTACTCTGTGCAAAAAACCTGGTGAAA AAGGATTTTTTCCGACTTCCTGATCCATTTGCTAAGGTGGTGGTTGATGGATCTGGGCAA TGCCATTCTACAGATACTGTGAAGAATACGCTTGATCCAAAGTGGAATCAGCATTATGAC CTGTATATTGGAAAGTCTGATTCAGTTACGATCAGTGTATGGAATCACAAGAAGATCCAT AAGAAACAAGGTGCTGGATTTCTCGGTTGTGTTCGTCTTCTTTCCAATGCCATCAACCGC CTCAAAGACACTGGTTATCAGAGGTTGGATTTATGCAAACTCGGGCCAAATGACAATGAT ACAGTTAGAGGACAGATAGTAGTAAGTCTTCAGTCCAGAGACCGAATAGGCACAGGAGGA CAAGTTGTGGACTGCAGTCGTTTATTTGATAACGATTTACCAGACGGCTGGGAAGAAAGG AGAACCGCCTCTGGAAGATCCAGTNTCTAAACCATATAAACAGAACTANCGCATGGGAGC GCCNAACACGACCGGCATCCCGATATTCTAGCCCTGGCAGACCTCTTAGCTGCTTTGNTG ATGAGAACACTCCCATTAGTGGAACAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022739 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022739.3, NP_073576.1 |
| RefSeq Size | 3866 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 64750 |
| UniProt ID | Q9HAU4 |
| Domains | C2, HECT, WW |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Protein Pathways | Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, TGF-beta signaling pathway, Type I diabetes mellitus, Ubiquitin mediated proteolysis, Viral myocarditis |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210866 | SMURF2 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) |
CNY 5,480.00 |
|
| RC210866L1 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), Myc-DDK-tagged |
CNY 7,880.00 |
|
| RC210866L2 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mGFP tagged |
CNY 7,700.00 |
|
| RC210866L3 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC210866L4 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2), mGFP tagged |
CNY 7,880.00 |
|
| RG210866 | SMURF2 (tGFP-tagged) - Human SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) |
CNY 7,080.00 |
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