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DEM1 (EXO5) (NM_022774) Human Untagged Clone
CAT＃: SC112422
EXO5 (untagged)-Human defects in morphology 1 homolog (S. cerevisiae) (DEM1)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C1orf176; DEM1; Exo V; hExo5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112422 sequence for NM_022774 edited (data generated by NextGen Sequencing)
ATGGCAGAGACAAGAGAAGAGGAGACAGTGTCAGCAGAAGCCTCAGGGTTCTCAGACTTG AGTGACTCAGAGTTCCTGGAGTTTCTGGACCTAGAAGATGCCCAAGAGTCAAAGGCTTTA GTTAACATGCCTGGCCCATCTTCTGAATCCCTTGGGAAGGATGACAAACCCATAAGCTTA CAAAACTGGAAAAGAGGATTGGATATATTATCACCCATGGAGAGATTCCACCTTAAATAT TTATATGTCACTGACCTGGCTACTCAGAACTGGTGTGAACTGCAAACAGCATATGGGAAG GAGCTTCCTGGTTTCTTGGCACCTGAGAAGGCAGCTGTGTTGGACACTGGTGCCAGCATA CACCTAGCTAGAGAACTAGAACTTCATGATCTTGTGACTGTCCCAGTCACCACTAAAGAA GATGCTTGGGCAATTAAGTTTCTGAACATACCTTTGCTGATTCCTACCCTGCAGTCAGAA GGGCACATCAGAGAGTTTCCAGTGTTTGGGGAAGGGGAGGGTGTACTTCTTGTTGGAGTG ATTGATGAGCTGCACTATACAGCCAAGGGGGAACTGGAGCTGGCGGAACTCAAGACACGC AGGCGCCCTATGCTCCCTCTGGAAGCTCAGAAGAAGAAAGACTGTTTTCAAGTCAGCCTA TACAAATATATCTTTGATGCCATGGTACAAGGAAAAGTGACCCCTGCTAGCCTAATCCAC CACACAAAGTTGTGTCTAGAAAAGCCACTGGGGCCATCAGTGCTGAGGCATGCCCAGCAG GGAGGCTTCTCTGTGAAGTCTTTGGGTGACCTCATGGAACTTGTCTTCTTGTCTCTAACA CTGTCAGACCTCCCAGTTATTGATATCTTGAAGATTGAGTATATCCACCAAGAGACTGCC ACTGTGCTGGGTACTGAGATTGTAGCCTTCAAAGAGAAGGAGGTGAGAGCCAAGGTGCAG CATTATATGGCCTACTGGATGGGCCACCGAGAGCCCCAGGGAGTTGACGTGGAGGAGGCT TGGAAGTGCCGGACGTGTACCTATGCAGACATTTGTGAGTGGAGAAAGGGCAGTGGAGTG CTCAGCTCTACACTGGCGCCCCAAGTCAAAAAAGCCAAATGA Clone variation with respect to NM_022774.1 452 t=>c >OriGene 5' read for NM_022774 unedited
ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGCCTTGTCATTTCAGCA TTCTGGCCTTCCCTATGAGGAGGAAGATCAACGAAGTCACAGGGCCCTTCTAGCCCAATC CAGAGCTGTACCATGGCAGAGACAAGAGAAGAGGAGACAGTGTCAGCAGAAGCCTCAGGG TTCTCAGACTTGAGTGACTCAGAGTTCCTGGAGTTTCTGGACCTAGAAGATGCCCAAGAG TCAAAGGCTTTAGTTAACATGCCTGGCCCATCTTCTGAATCCCTTGGGAAGGATGACAAA CCCATAAGCTTACAAAACTGGAAAAGAGGATTGGATATATTATCACCCATGGAGAGATTC CACCTTAAATATTTATATGTCACTGACCTGGCTACTCAGAACTGGTGTGAACTGCAAACA GCATATGGGAAGGAGCTTCCTGGTTTCTTGGCACCTGAGAAGGCAGCTGTGTTGGACACT GGTGCCAGCATACACCTAGCTAGAGAACTAGAACTTCATGATCTTGTGACTGTCCCAGTC ACCACTAAAGAAGATGCTTGGGCAATTAAGTTTCTGAACATACCTTTGCTGATTCCTACC CTGCAGTCAGAAGGGCACATCAGAGAGTTTCCAGTGTTTGGGGAAGGGGAGGGTGTACTT CTTGTTGGAGTGATTGATGAGCTGCACTATACAGCCAAGGNGGAACTGGAGCTGGCGGAA CTCAAGACACGCAGGCGCCCTATGCTCCCTCTGGAAGCTCANAAGAGAAAGACTGTTNTC AAGTCAGCCTATACAAATATATCTTTGATGCCATGGTACAGGGAAAAGTGACCCCTGCTA GCCTAATCCACACANCAAGNTGTGTCTANAAAAGCACTGGGGCCATCANTGCTGAGGCAT GCCCANCAGGNNNAGCTCTCTGTGAAGTCTTGGGTGACTCATGGNACTG >OriGene 3' read for NM_022774 unedited
ATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGGACAGCATTTC ATTTTATTATGTAACTGTAGAAAGCCTTGATCAAGATAAAAATAGGGATGACTTATCAGA AACTGAAGAATTTTCTTAAGAAAGCAAAGTTTACTGAAGGATACCTTCATTCCAGCCATG ATGAGCATCTGTCTTCTCAGGCAATCATGATGAAGCTCCAGGGACAGTATAACCCATCTC TCCCACTCATCCCTGAGCCTTGGTCCTGGACTGAATGTGGTTAGAGGTTGTGGAAATAAA AAAAAGAACCAAAATAAGAACACTCTCCATAAAAGCCAAGCTCAGAGACTGGTCCTCTTT TGCTTAAGTACAACAGGAGCAGGAAGGATCAACATTCTTGAAAGCATACCTTCTATTCAT TTGGCTTTTTTGACTTGGGGCGCCAGTGTAGAGCTGAGCACTCCACTGCCCTTTCTCCAC TCACAAATGTCTGCATAAGTACACGTCCGGCACTTCCAAGCCTCCTCCACGTCAACTCCC TGGGGCTCTCGGTGGCCCATCCAGTAAGCCATATAATGCTGCACCTTGGCTCTCACCTCC TTCTCTTTGAAAGCTACAATCTCAGTACCCAGCACAGTGGCAGTCTCTTGGAGGATATAC TCAATCTTCAAGATATCAATAACTGGGAAGGCTGACAGTGTTAGAGACAAGAAGACAAGT TCCATGAGGCCACCCCAAGACTTCACAGAGAATCCTCCCTGCCTGGCATGGCTCAACACT GATGGCCCCAAGGGCTTTTCTAGACACCACTTTGTGTGGAGGATTATGCCTAACAGGGGT AATTTTTCCTTGTACCACTNGCTCCAAGATTATTTGGGCTAAGCCGCCTGAAAAACTCTC TCTTCTTTCGACTCCAAAGGAACATATGGCCCCGGTTGTCCTGAATTCCCACTTTCATTT CCCCTGGGCTGTTATGGGCCTTACATACCTCAACAAAATCCCCCCCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022774 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022774.1, NP_073611.1 |
| RefSeq Size | 2217 bp |
| RefSeq ORF | 1122 bp |
| Locus ID | 64789 |
| UniProt ID | Q9H790 |
| Gene Summary | The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203370 | EXO5 (Myc-DDK-tagged)-Human defects in morphology 1 homolog (S. cerevisiae) (DEM1) |
CNY 3,656.00 |
|
| RC203370L3 | Lenti ORF clone of Human defects in morphology 1 homolog (S. cerevisiae) (DEM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203370L4 | Lenti ORF clone of Human defects in morphology 1 homolog (S. cerevisiae) (DEM1), mGFP tagged |
CNY 5,890.00 |
|
| RG203370 | EXO5 (tGFP-tagged) - Human defects in morphology 1 homolog (S. cerevisiae) (DEM1) |
CNY 5,256.00 |
|
| SC320907 | EXO5 (untagged)-Human defects in morphology 1 homolog (S. cerevisiae) (DEM1) |
CNY 3,656.00 |
