PPCS (NM_024664) Human Untagged Clone
CAT#: SC112103
PPCS (untagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1
CNY 6,270.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CMD2C |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_024664, the custom clone sequence may differ by one or more nucleotides
ATGGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTGCTGCGCGCTGGGCTGAGGTTATGG CTCGCTTCGCGGCCAGGCTGGGCGCGCAGGGCCGGCGGGTGGTGTTGGTTACGTCAGGCGGCACCAAGGT CCCACTGGAAGCGCGGCCGGTGCGCTTCCTGGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCC GAGGCCTTCCTAGCCGCCGGCTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGCCC ACCGCTTCCCACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTCGGGCTTGCTGAG CCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAGGAGCTACCAGGAGGCTGCGGCT GCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGCGGACTATTTGCATCTGTTGCAGGCTGCGGCCC AGGCACTCAATCCGCTAGGCCCTTCTGCGATGTTTTACCTGGCTGCGGCTGTGTCAGATTTCTATGTTCC TGTCTCTGAAATGCCTGAACACAAGATCCAGTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTG CCAAAACTGCTTTCTCCTTTGGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGAGA CTGACCCCGCCATTGTAATTAATCGAGCTCGGAAGGCTTTGGAAATTTATCAGCATCAAGTGGTGGTGGC TAATATCCTTGAGTCACGACAGTCCTTTGTGTTTATTGTAACCAAAGACTCGGAAACCAAGTTATTGCTA TCAGAGGAAGAAATAGAAAAAGGCGTAGAGATAGAAGAGAAGATAGTGGATAATCTTCAGTCTCGACACA CAGCTTTTATAGGTGACAGAAACTGA >OriGene 5' read for NM_024664 unedited
NGTACCGTCAAATCATTGTAAAACGACTCACTATAGGCGGCCGCGACATCGGCACGAGGC CTCGTGCCGAATTCGGCACGAGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTGCTGC GCGCTGGGCTGAGGTTATGGCTCGCTTCGCGGCCAGGCTGGGCGCGCAGGGCCGGCGGGT GGTGTTGGTTACGTCAGGCGGCACCAAGGTCCCACTGGAAGCGCGGCCGGTGCGCTTCCT GGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCCGAGGCCTTCCTAGCCGCCGG CTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGCCCACCGCTTCCC ACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTCGGGCTTGCTGAG CCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAGGAGCTACCAGGA GGCTGCGGCTGCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGCGGACTATTTGCA TCTGTTGCAGGCTGCGGCCCAGGCACTCAATCCGCTAGGCCCTTCTGCGATGTTTTACCT GGCTGCGGCTGTGTCAGATTTCTATGTTCCTGTCTCTGAAATGCCTGAACACAAGATCCA GTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTGCCAAAACTGCTTTCTCCTTT GGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGAGACTGACCCCGC CATTTGTATTAATCGAGCTCGGAAGGCTTTTGAAATTTATCAGCATCAAGTGGTGGTGGC TAATATCCTTGAGTCACGACAGTCCTTTGTGGTTATTGTACC >OriGene 3' read for NM_024664 unedited
AAATTANGTGGTGGGNCACCACTCTTTTGTCCTTGNATTTTTTATACATCCATTTCCTAA ACAGATATGTTTATTCATCTTTTTAATCCTCATGGCTCAGCACAGGGGCCTGACATACAT AGGCATTCAACCAATATTTGATCTCCAAAGCATGACCATTCATGCATCCATAATCCTTTT TCTATAGTATAAAGATGGCTGACATTCAGGCCCCCATGTATATTTTAAGGAATATATAAT TCCCAATAAGCTCTTCTTTTTAAAGGTGATTAACAGTTCTAGTGTTCAATTTCAAAATCA AAATGACATCATATCAGGTGTCATTAAAAGACAAATGCCAAACCATATTTGCCTACACAC CACTGCCTTTTCCCTTTCTTTCATTTTATCTGTCCATATGAAAGCCATGGTTTTTTTTGT AGTTTTCACCATCTCTAAGAGCCCTGAACAATTTTTGATCCTATAAGGGCTTTTTACTTC AGTTTCTGTCACCTATAAAAGCTGTGTGTCGAGACTGAAGATTATCCACTATCTTCTCTT CTATCTCTACGCCTTTTCCTATTTCTTCCTCTGATAGCAATACCTTGGTTTCCGAGTCTT TGGTTACAATAAACACAAATGACTGTCGTGACTCAAGGATATTACCCCCCACCACTTGAT GCTGATAAATCTCCAAAGCCTTCCGAGCTCGATTAATTACAATGGCGGCGTCAGTCTCCC CCTCAAAGGAAATTATAAATGCTTTGGGAACCCAATCTTAACCAAAGAGAAAGCCTTTTT GGCCCACCCTCATTGTTATCTGCATTGGGTCCCCACATGCCTGCACCTCGCGCCCTGGCA TTCCATATCCTCACCATATAACCTGACCCAGTCCCACCTGGCAAAAATTN |
Restriction Sites | NotI-NotI |
ACCN | NM_024664 |
Insert Size | 1360 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_024664.1, NP_078940.1 |
RefSeq Size | 2312 bp |
RefSeq ORF | 855 bp |
Locus ID | 79717 |
UniProt ID | Q9HAB8 |
Domains | dfp |
Protein Pathways | Metabolic pathways, Pantothenate and CoA biosynthesis |
Gene Summary | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212317 | PPCS (Myc-DDK-tagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1 |
CNY 2,400.00 |
|
RC212317L3 | Lenti ORF clone of Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212317L4 | Lenti ORF clone of Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG212317 | PPCS (tGFP-tagged) - Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1 |
CNY 4,370.00 |
|
SC317467 | PPCS (untagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1 |
CNY 6,270.00 |