PPCS (NM_024664) Human Untagged Clone

CAT#: SC112103

PPCS (untagged)-Human phosphopantothenoylcysteine synthetase (PPCS), transcript variant 1



  "NM_024664" in other vectors (5)

CNY 6,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
PPCS Antibody - N-terminal region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CMD2C
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_024664, the custom clone sequence may differ by one or more nucleotides


ATGGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTGCTGCGCGCTGGGCTGAGGTTATGG
CTCGCTTCGCGGCCAGGCTGGGCGCGCAGGGCCGGCGGGTGGTGTTGGTTACGTCAGGCGGCACCAAGGT
CCCACTGGAAGCGCGGCCGGTGCGCTTCCTGGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCC
GAGGCCTTCCTAGCCGCCGGCTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGCCC
ACCGCTTCCCACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTCGGGCTTGCTGAG
CCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAGGAGCTACCAGGAGGCTGCGGCT
GCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGCGGACTATTTGCATCTGTTGCAGGCTGCGGCCC
AGGCACTCAATCCGCTAGGCCCTTCTGCGATGTTTTACCTGGCTGCGGCTGTGTCAGATTTCTATGTTCC
TGTCTCTGAAATGCCTGAACACAAGATCCAGTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTG
CCAAAACTGCTTTCTCCTTTGGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGAGA
CTGACCCCGCCATTGTAATTAATCGAGCTCGGAAGGCTTTGGAAATTTATCAGCATCAAGTGGTGGTGGC
TAATATCCTTGAGTCACGACAGTCCTTTGTGTTTATTGTAACCAAAGACTCGGAAACCAAGTTATTGCTA
TCAGAGGAAGAAATAGAAAAAGGCGTAGAGATAGAAGAGAAGATAGTGGATAATCTTCAGTCTCGACACA
CAGCTTTTATAGGTGACAGAAACTGA


>OriGene 5' read for NM_024664 unedited
NGTACCGTCAAATCATTGTAAAACGACTCACTATAGGCGGCCGCGACATCGGCACGAGGC
CTCGTGCCGAATTCGGCACGAGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTGCTGC
GCGCTGGGCTGAGGTTATGGCTCGCTTCGCGGCCAGGCTGGGCGCGCAGGGCCGGCGGGT
GGTGTTGGTTACGTCAGGCGGCACCAAGGTCCCACTGGAAGCGCGGCCGGTGCGCTTCCT
GGACAACTTCAGCAGCGGGCGGCGCGGTGCAACCTCGGCCGAGGCCTTCCTAGCCGCCGG
CTACGGGGTCCTGTTCTTGTATCGCGCTCGCTCTGCCTTCCCCTATGCCCACCGCTTCCC
ACCCCAGACTTGGCTGTCCGCTCTGCGGCCTTCGGGCCCAGCCCTTTCGGGCTTGCTGAG
CCTGGAGGCCGAGGAGAATGCACTTCCGGGTTTTGCTGAGGCTCTGAGGAGCTACCAGGA
GGCTGCGGCTGCAGGCACCTTCCTGGCAGTAGAGTTCACCACTTTGGCGGACTATTTGCA
TCTGTTGCAGGCTGCGGCCCAGGCACTCAATCCGCTAGGCCCTTCTGCGATGTTTTACCT
GGCTGCGGCTGTGTCAGATTTCTATGTTCCTGTCTCTGAAATGCCTGAACACAAGATCCA
GTCATCTGGGGGCCCACTGCAGATAACAATGAAGATGGTGCCAAAACTGCTTTCTCCTTT
GGTTAAAGATTGGGCTCCCAAAGCATTTATAATTTCCTTTAAGTTGGAGACTGACCCCGC
CATTTGTATTAATCGAGCTCGGAAGGCTTTTGAAATTTATCAGCATCAAGTGGTGGTGGC
TAATATCCTTGAGTCACGACAGTCCTTTGTGGTTATTGTACC
>OriGene 3' read for NM_024664 unedited
AAATTANGTGGTGGGNCACCACTCTTTTGTCCTTGNATTTTTTATACATCCATTTCCTAA
ACAGATATGTTTATTCATCTTTTTAATCCTCATGGCTCAGCACAGGGGCCTGACATACAT
AGGCATTCAACCAATATTTGATCTCCAAAGCATGACCATTCATGCATCCATAATCCTTTT
TCTATAGTATAAAGATGGCTGACATTCAGGCCCCCATGTATATTTTAAGGAATATATAAT
TCCCAATAAGCTCTTCTTTTTAAAGGTGATTAACAGTTCTAGTGTTCAATTTCAAAATCA
AAATGACATCATATCAGGTGTCATTAAAAGACAAATGCCAAACCATATTTGCCTACACAC
CACTGCCTTTTCCCTTTCTTTCATTTTATCTGTCCATATGAAAGCCATGGTTTTTTTTGT
AGTTTTCACCATCTCTAAGAGCCCTGAACAATTTTTGATCCTATAAGGGCTTTTTACTTC
AGTTTCTGTCACCTATAAAAGCTGTGTGTCGAGACTGAAGATTATCCACTATCTTCTCTT
CTATCTCTACGCCTTTTCCTATTTCTTCCTCTGATAGCAATACCTTGGTTTCCGAGTCTT
TGGTTACAATAAACACAAATGACTGTCGTGACTCAAGGATATTACCCCCCACCACTTGAT
GCTGATAAATCTCCAAAGCCTTCCGAGCTCGATTAATTACAATGGCGGCGTCAGTCTCCC
CCTCAAAGGAAATTATAAATGCTTTGGGAACCCAATCTTAACCAAAGAGAAAGCCTTTTT
GGCCCACCCTCATTGTTATCTGCATTGGGTCCCCACATGCCTGCACCTCGCGCCCTGGCA
TTCCATATCCTCACCATATAACCTGACCCAGTCCCACCTGGCAAAAATTN
Restriction Sites NotI-NotI     
ACCN NM_024664
Insert Size 1360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024664.1, NP_078940.1
RefSeq Size 2312 bp
RefSeq ORF 855 bp
Locus ID 79717
UniProt ID Q9HAB8
Domains dfp
Protein Pathways Metabolic pathways, Pantothenate and CoA biosynthesis
Gene Summary Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]
Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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