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NOTUM (NM_178493) Human Untagged Clone
CAT＃: SC111889
NOTUM (untagged)-Human notum pectinacetylesterase homolog (Drosophila) (NOTUM)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hNOTUM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_178493, the custom clone sequence may differ by one or more nucleotides
ATGGGCCGAGGGGTGCGCGTGCTGCTGCTGCTGAGCCTGCTGCACTGCGCCGGGGGCAGCGAGGGCAGGA AGACCTGGCGGCGCCGGGGTCAGCAGCCGCCTCCTCCCCCGCGGACCGAGGCGGCGCCGGCGGCCGGACA GCCCGTGGAGAGCTTCCCGCTGGACTTCACGGCCGTGGAGGGTAACATGGACAGCTTCATGGCGCAAGTC AAGAGCCTGGCGCAGTCCCTGTACCCCTGCTCCGCGCAGCAGCTCAACGAGGACCTGCGCCTGCACCTCC TACTCAACACCTCGGTGACCTGCAACGACGGCAGCCCCGCCGGCTACTACCTGAAGGAGTCCAGGGGCAG CCGGCGGTGGCTCCTCTTCCTGGAAGGCGGCTGGTACTGCTTCAACCGCGAGAACTGCGACTCCAGATAC GACACCATGCGGCGCCTCATGAGCTCCCGGGACTGGCCGCGCACTCGCACAGGCACAGGGATCCTGTCCT CACAGCCGGAGGAGAACCCCTACTGGTGGAACGCAAACATGGTCTTCATCCCCTACTGCTCCAGTGATGT TTGGAGCGGGGCTTCATCCAAGTCTGAGAAGAACGAGTACGCCTTCATGGGCGCCCTCATCATCCAGGAG GTGGTGCGGGAGCTTCTGGGCAGAGGGCTGAGCGGGGCCAAGGTGCTGCTGCTGGCCGGGAGCAGCGCGG GGGGCACCGGGGTGCTCCTGAATGTGGACCGTGTGGCTGAGCAGCTGGAGAAGCTGGGCTACCCAGCCAT CCAGGTGCGAGGCCTGGCTGACTCCGGCTGGTTCCTGGACAACAAGCAGTATCGCCACACAGACTGCGTC GACACGATCACGTGCGCGCCCACGGAGGCCATCCGCCGTGGCATCAGGTACTGGAACGGGGTGGTCCCGG AGCGCTGCCGACGCCAGTTCCAGGAGGGCGAGGAGTGGAACTGCTTCTTTGGCTACAAGGTCTACCCGAC CCTGCGCTGCCCTGTGTTCGTGGTGCAGTGGCTGTTTGACGAGGCACAGCTGACGGTGGACAACGTGCAC CTGACGGGGCAGCCGGTGCAGGAGGGCCTGCGGCTGTACATCCAGAACCTCGGCCGCGAGCTGCGCCACA CACTCAAGGACGTGCCGGCCAGCTTTGCCCCCGCCTGCCTCTCCCATGAGATCATCATCCGGAGCCACTG GACGGATGTCCAGGTGAAGGGGACGTCGCTGCCCCGAGCACTGCACTGCTGGGACAGGAGCCTCCATGAC AGCCACAAGGCCAGCAAGACCCCCCTCAAGGGCTGCCCCGTCCACCTGGTGGACAGCTGCCCCTGGCCCC ACTGCAACCCCTCATGCCCCACCGTCCGAGACCAGTTCACGGGGCAAGAGATGAACGTGGCCCAGTTCCT CATGCACATGGGCTTCGACATGCAGACGGTGGCCCAGCCGCAGGGACTGGAGCCCAGTGAGCTGCTGGGG ATGCTGAGCAACGGAAGCTAG >OriGene 5' read for NM_178493 unedited
NNNNAATGTTCANAATTTTGTNAATACGAACTNCATNATAGGGNNCGGCCGCGNNAATTC GGCACCAGAANCTCAACGAGGACCTGCGCCTGCACCTCCTACTCAACACCTCGGTGACCT GCAACGACGGCAGCCCCGCCGGCTACTACCTGAAGGAGTCCAGGGGCAGCCGGCGGTGGC TCCTCTTCCTGGAAGGCGGCTGGTACTGCTTCAACCGCGAGAACTGCGACTCCAGATACG ACACCATGCGGCGCCTCATGAGCTCCCGGGACTGGCCGCGCACTCGCACAGGCACAGGGA TCCTGTCCTCACAGCCGGAGGAGAACCCCTACTGGTGGAACGCAAACATGGTCTTCATCC CCTACTGCTCCAGTGATGTTTGGAGCGGGGCTTCATCCAAGTCTGAGAAGAACGAGTACG CCTTCATGGGCGCCCTCATCATCCAGGAGGTGGTGCGGGAGCTTCTGGGCAGAGGGCTGA GCGGGGCCAAGGTGCTGCTGCTGGCCGGGAGCAGCGCGGGGGGCACCGGGGTGCTCCTGA ATGTGGACCGTGTGGCTGAGCAGCTGGAGAAGCTGGGCTACCCAGCCATCCAGGTGCGAG GCCTGGCTGACTCCGGCTGGTTCCTGGACAACAAGCAGTATCGCCACACAGACTGCGTCG ACACGATCACGTGCGCGCCCACGGAGGCCATCCGCCGTGGCATCAGGTACTGGAACGGNG TGGTCCCGGAGCGCTGCCGACGCCAGTTCCAGGAAGGCGAGGAGTGGAACTGCTTCTTTG GCTACAAGGTCTACCCGACCCTGCGCTGCCCTGTGTTCGTGGTGCAGTGGCTGTTTGACG AGCACAGCTGACGGTGGACACGTGCACCTGACGGGGCAGCCGTGCCCAGAGGCCTGCNGC TGTACATCCAGAACCTCGGCCGCGAGCTGCGCCAACACTCAAGGACGTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_178493 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178493.3, NP_848588.2 |
| RefSeq Size | 2281 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 147111 |
| UniProt ID | Q6P988 |
| Gene Summary | Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209331 | NOTUM (Myc-DDK-tagged)-Human notum pectinacetylesterase homolog (Drosophila) (NOTUM) |
CNY 5,488.00 |
|
| RC209331L1 | Lenti ORF clone of Human notum pectinacetylesterase homolog (Drosophila) (NOTUM), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC209331L2 | Lenti ORF clone of Human notum pectinacetylesterase homolog (Drosophila) (NOTUM), mGFP tagged |
CNY 5,890.00 |
|
| RC209331L3 | Lenti ORF clone of Human notum pectinacetylesterase homolog (Drosophila) (NOTUM), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209331L4 | Lenti ORF clone of Human notum pectinacetylesterase homolog (Drosophila) (NOTUM), mGFP tagged |
CNY 5,890.00 |
|
| RG209331 | NOTUM (tGFP-tagged) - Human notum pectinacetylesterase homolog (Drosophila) (NOTUM) |
CNY 7,088.00 |
|
| SC327883 | NOTUM (untagged)-Human notum pectinacetylesterase homolog (Drosophila) (NOTUM) |
CNY 5,856.00 |
