TRIM5 alpha (TRIM5) (NM_033034) Human Untagged Clone
CAT#: SC111855
TRIM5 (untagged)-Human tripartite motif containing 5 (TRIM5), transcript variant alpha
CNY 3,656.00
CNY 7,890.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | RNF88; TRIM5alpha |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_033034 edited
GAATTCGGCACCAGCCAGGACAGGCAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAAT CCTGGTTAATGTAAAGGAGGAGGTGACCTGCCCCATCTGCCTGGAACTCCTGACACAACC CCTGAGCCTGGACTGCGGCCACAGCTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAA GTCCATGCTAGACAAAGGAGAGAGTAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGA GAACATACGGCCTAATCGGCATGTAGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTT GAGCCCAGAGGGGCAGAAAGTTGATCATTGTGCACGCCATGGAGAGAAACTTCTACTCTT CTGTCAGGAGGACGGGAAGGTCATTTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGG TCACCACACGTTCCTCACAGAGGAGGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGC TCTGGAGATGCTGAGGCAGAAGCAGCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGA AGAGAAAGCTTCCTGGAAGACTCAAATACAGTATGACAAAACCAACGTCTTGGCAGATTT TGAGCAACTGAGAGACATCCTGGACTGGGAGGAGAGCAATGAGCTGCAAAACCTGGAGAA GGAGGAGGAAGACATTCTGAAAAGCCTTACGAACTCTGAAACTGAGATGGTGCAGCAGAC CCAGTCCCTGAGAGAGCTCATCTCAGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGA GCTGCTTCAGGGTGTGGATGGCGTCATAAAAAGAGCTGACAGATGTCCGACGCTACTGGG TTGATGTGACAGTGGCTCCAAACAACATTTCATGTGCTGTCATTTCTGAAGATAAGAGAC AAGTGAGCTCTCCGAAACCACAGATAATATATGGGGCACGAGGGACAAGATACCAGACAT TTGTGAATTTCAATTATTGTACTGGCATCCTGGGCTCTCAAAGTATCACATCAGGGAAAC ATTACTGGGAGGTAGACGTGTCCAAGAAAACTGCTTGGATCCTGGGGGTATGTGCTGGCT TCCAACCTGATGCAATGTGTAATATTGAAAAAAATGAAAATTATCAACCTAAATACGGCT ACTGGGTTATAGGGTTAGAGGAAGGAGTTAAATGTAGTGCTTTCCAGGATAGTTCCTTCC ATACTCCTTCTGTTCCTTTCATTGTGCCCCTCTCTGTGATTATTTGTCCTGATCGTGTTG GAGTTTTCCTAGACTATGAGGCTTGCACTGTCTCATTCTTCAATATCACAAACCATGGAT TTCTCATCTATAAGTTTTCTCACTGTTCTTTTTCTCAGCCTGTATTTCCATATTTAAATC CTAGAAAATGTGGAGTCCCCATGACTCTGTGCTCACCAAGCTCTTGAACCTTCTTACACA CTCAGCCCCTTCTGTACAGCACCTCTTGTCCAGGTGCATCTCATACACCTGAACTCATTT GCATCATTTTAACCATCTTTTCCTTGCTGTCTCCCTTCTTTCTATTTGAACGTCCTTCAC TCATCAGTAAAATGTAATAATTGCCTTGTGCCATATTGTCCCCAATATTTTATTGACATT TGATAGCAATTTTTTTCATCATTTTCCGTACTCCTAAGGAAAACTGACCTATACCTCATA AAATGAGACCGCTATTTAGGTATTACTTCTGCCAGATATTTATCACCCAATTGCCTCTGA CACTGACTAAGAAGATGAAGAAAAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATAA TTGTTCACCAATGAATGAGTCCTTAGCCCTGTGTCAGTTTACCCTCGATGCCCTTATTTG TGAGTTAAAGAGAAAATATCATAAATGGTATACTCTTAAGTATAGAGGTTTTGTATCTAG AGGATCTCAGTTCAACTCCTGTCTCTCCATATACCAGCAGTGTAACTGTGAATAACATAC TTAAATGGCTGTGCTTATTTCCTTTTCTTTTCTTTTTTCTTTTTTTTTTTTTTTGAGATG AAGTTTTGCTCTTGTTCCCCAGGCTGGAGTGCAATGGCACGATCTCGGTTCACTGCAACC TCCACCTCTCAGATTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAG GTGCCCACCACCACCCCTGGCTAAATTTGTATTTTCAGTAGAGACGGGGTTTCCCCATGT TGGTTAGGCTCGTCTAGAACCTCTGACCTCAGGTGATCCACCCGCCTCGGCCTCCCAAAG TGCTGGGATTACAGGCGTGAGCCACGGCGCCCAGCCTGTGCTTATTTTCTTAAAATAATT TTTGTATTAAAAACTTCACATTAAATAAGTGCTAATGTTTTATTGCATAGTAGGGTGACT AGAGTTAACAATAACCTATTGCATATATTTTGAAATAGCTAGAAGAGAGGATTTTGAAAG TTCTCAACACAAAGAAATGACACATATTTGAGGTGATGGATATGCTAATTACCCTGGTTC GGTTATTACGCAATGTATACATGTATCAAAACATCACACTGTACCACATAAATATGTATA TTTATTATTTGTCAATTAAAAGCAAAATAAAACAAAAAACCTTCATCTAATACTTTGGAT CATTGTGAAAAAATAAATTCCTGAAGTATAAAGCATCTXXXXXXXXXXXXXXXXAACTCG AC >OriGene 5' read for NM_033034 unedited
CAAATTTATGTATACGACTCACTATAGGGCGGCCGCGNAATTCGCACCAGCCAGGACAGG CAGGAGCAGTGGAATAGCTAGTATGGCTTCTGGAATCCTGGTTAATGTAAAGGAGGAGGT GACCTGCCCCATCTGCCTGGAACTCCTGACACAACCCCTGAGCCTGGACTGCGGCCACAG CTTCTGCCAAGCATGCCTCACTGCAAACTACAAGAAGTCCATGCTAGACAAAGGAGAGAG TAGCTGCCCTGTGTGCCGGATCAGTTACCAGCCTGAGAACATACGGCCTAATCGGCATGT AGCCAACATAGTGGAGAAGCTCAGGGAGGTCAAGTTGAGCCCAGAGGGGCAGAAAGTTGA TCATTGTGCACGCCATGGAGAGAAACTTCTACTCTTCTGTCAGGAGGACGGGAAGGTCAT TTGCTGGCTTTGTGAGCGGTCTCAGGAGCACCGTGGTCACCACACGTTCCTCACAGAGGA GGTTGCCCGGGAGTACCAAGTGAAGCTCCAGGCAGCTCTGGAGATGCTGAGGCAGAAGCA GCAGGAAGCTGAAGAGTTAGAAGCTGACATCAGAGAAGAGAAAGCTTCCTGGAAGACTCA AATACAGTATGACAAAACCAACGTCTTGGCAGATTNTGAGCAACTGAGAGACATCCTGGA CTGNGAGGAGAGCAATGAGCTGCANAACCTGNAGAAAGAGGANGAAGACATTCTGAAAAG CCTTACGAACTCTGAAACTGAGATGGTGCAGCAGACCCAGTCCCTGAGAGAGCTCATCTC AGATCTGGAGCATCGGCTGCAGGGGTCAGTGATGGAGCTGCCTCAAGGTGTTGGATTGCG TCATAAAAAGAGCTGACAGATGTCCGACGCTACTNGGGTTGATGTGACAGTGGCTCCAAA CAACATTAATGGGCTGCCATTCCTGAGAATAGAGAAAGGGAGCCTTCGGAACCCAGTAAA TATATGG >OriGene 3' read for NM_033034 unedited
TTGGACCGCGGCCGCAATCTACAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTAAAAGGCTTAAAACTTCGAGGAATTTATTTTTTACCAAGGATCCC AAAGTTTTAAAGGAGGGGTTTTGGTTTAATTTGGCTTTAAATGGCCAAAAAAAAAATATC CCTATTTATGGGGCCCAGGGGGAGGTTTTGAAACATGTTTACTTTGGGAAAAACCCGACC CAGGGGAATAACCAAACCCTTTTTTTTAAAAAGGGGCCATTTTTTTGGGGGGAAAACTTT AAAAATCCTTTTTTTTAGCTATTCCAAAATATATGCAAAAGGTTATTGTTAACCTTAGCC CCCCTCCTTTGCAAAAAAACATTCCCCCTTATTTAAGGGGAAGCTTTTAAATCAAAAATT ATTTTAAAAAAAAAACCCCCGGCTGGGCCCCGGGGCTCCCCCCTGAAACCCCACACTTGG GGAGGCCCAGGCGGGGGGACCCCCTGGGGTCACAGGCTCAACACCACCCCCACACACATG GGGCACACCCCCCCTTCCAATGAACCACCAACCCCAACCACCGGGTGGGGGGGGGCCCCT CCACACCCCCCCCTGGCGCGGCCCGCCCCGAACACCCTTCTCTCCCTCCACCCGAGCGGC CACACCACCCCACAACCTCCCCCCTCCCCCCACACCGGCCCCATCCACCCACCATCTTTC TCTCTCCCCCTCAATCCCAACCCCTCCTTTACGCCCACCCCCCTCTCCTCCCCCCCTTCC TCCCCCCCCACCCCTTCCCCTATCTCACCACCCCCCGCTAACAACTCCCTCACTCACACT CCCCCCCCCCCCCCCCAAACCACCCTCCCCCCCCCCCCCCCCCCAAAACCCAATATCCTC CCCCCTCCCCCCCCATACCCATTCCCCCACCCCCCCCACCTTACTCTACCCCACTTCCAC CCCCCACTCCCTCCCCCCACCCCCCCACCCCCTCCT |
Restriction Sites | NotI-NotI |
ACCN | NM_033034 |
Insert Size | 2750 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_033034.1, NP_149023.1 |
RefSeq Size | 2990 bp |
RefSeq ORF | 1482 bp |
Locus ID | 85363 |
UniProt ID | Q9C035 |
Domains | zf-B_box, RING, SPRY |
Protein Families | Druggable Genome |
Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009] Transcript Variant: This variant (alpha) encodes the longest isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212800 | TRIM5 (Myc-DDK-tagged)-Human tripartite motif containing 5 (TRIM5), transcript variant alpha |
CNY 3,656.00 |
|
RC212800L1 | Lenti ORF clone of Human tripartite motif containing 5 (TRIM5), transcript variant alpha, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC212800L2 | Lenti ORF clone of Human tripartite motif containing 5 (TRIM5), transcript variant alpha, mGFP tagged |
CNY 5,890.00 |
|
RC212800L3 | Lenti ORF clone of Human tripartite motif containing 5 (TRIM5), transcript variant alpha, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212800L4 | Lenti ORF clone of Human tripartite motif containing 5 (TRIM5), transcript variant alpha, mGFP tagged |
CNY 5,890.00 |
|
RG212800 | TRIM5 (tGFP-tagged) - Human tripartite motif containing 5 (TRIM5), transcript variant alpha |
CNY 5,256.00 |
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