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SPTLC1 (NM_006415) Human Untagged Clone
CAT＃: SC111752
SPTLC1 (untagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1
CNY 3,656.00
CNY 7,510.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111752 sequence for NM_006415 edited (data generated by NextGen Sequencing)
ATGGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCT CCTGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTC TCTAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTG ATTGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTC AACTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGT ATAAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCA GCTTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGC ACATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAA GCCATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAA AGAGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTTTGCTATTCAGAAAGGATTACAG GCATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTA CTAAAAGAACAAGAGATCGAAGATCAAAAGAATCCTCGCAAGGCTCGTGTAACTCGGCGT TTCATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAATTG GTTAAGTTAAAATACAAATACAAAGCAAGAATCTTCCTGGAGGAAAGCCTTTCATTTGGA GTCCTAGGAGAGCATGGCCGAGGAGTCACTGAACACTATGGAATCAATATTGATGATATT GATCTTATCAGTGCCAACATGGAGAATGCACTTGCTTCTATTGGAGGTTTCTGCTGTGGC AGGTCTTTTGTAATTGACCATCAGCGACTTTCCGGCCAGGGATACTGCTTTTCAGCTTCG TTACCTCCCCTGTTAGCTGCTGCAGCAATTGAGGCCCTCAACATCATGGAAGAGAATCCA GGTATTTTTGCAGTGTTGAAGGAAAAGTGCGGACAAATTCATAAAGCTTTACAAGGCATT TCTGGATTAAAAGTGGTGGGGGAGTCCCTTTCTCCAGCCTTTCACCTACAACTGGAAGAG AGCACTGGGTCTCGCGAGCAAGATGTCAGACTGCTTCAGGAAATTGTAGATCAATGCATG AACAGAAGTATTGCATTAACTCAGGCGCGCTACTTGGAGAAAGAAGAGAAGTGTCTCCCT CCTCCCAGCATTCGGGTTGTGGTCACGGTGGAACAAACAGAGGAAGAACTGGAGAGAGCT GCGTCCACCATCAAGGAGGTAGCCCAGGCCGTCCTGCTCTAG Clone variation with respect to NM_006415.2 >OriGene 5' read for NM_006415 unedited
GGGTTCANAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTAACTAT GGCGACCGCCACGGAGCAGTGGGTTCTGGTGGAGATGGTACAGGCGCTTTACGAGGCTCC TGCTTACCATCTTATTTTGGAAGGGATTCTGATCCTCTGGATAATCAGACTTCTTTTCTC TAAGACTTACAAATTACAAGAACGATCTGATCTTACAGTCAAGGAAAAAGAAGAACTGAT TGAAGAGTGGCAACCAGAACCTCTTGTTCCTCCTGTCCCAAAAGACCATCCTGCTCTCAA CTACAACATCGTTTCAGGCCCTCCAAGCCACAAAACTGTGGTGAATGGAAAAGAATGTAT AAACTTCGCCTCATTTAATTTTCTTGGATTGTTGGATAACCCTAGGGTTAAGGCAGCAGC TTTAGCATCTCTAAAGAAGTATGGCGTGGGGACTTGTGGACCCAGAGGATTTTATGGCAC ATTTGATGTTCATTTGGATTTGGAAGACCGCCTGGCAAAATTTATGAAGACAGAAGAAGC CATTATATACTCATATGGATTTGCCACCATAGCCAGTGCTATTCCTGCTTACTCTAAAAG AGGGGACATTGTTTTTGTAGATAGAGCTGCCTGCTNTGCTATTCAGAAAGGATTACAGGC ATCCCGTAGTGACATTAAGTTATTTAAGCATAATGACATGGCTGACCTCGAGCGACTACT AAAAGAACCAAGAGATCGAGATCAAAAGAATCCTCGCAGGGCTCGTGTAACTCGGCGTTT CATTGTAGTAGAAGGATTGTATATGAATACTGGAACTATTTGTCCTCTTCCAGAAATTGG TAAGTTAAAATACANATTCAAAGCAAGAATCTTTCTGGNAGGAAAGNCCTTTCATTTGNN AGTCCCTAGAGAGCATGGNCCGAGNAGTCACTGNACN >OriGene 3' read for NM_006415 unedited
ACCGCGGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGGAATTAGGTTTAT TTCATTAAAAAATAAAACAAAACAAACCAGACTCATCCCCCAAAGAGTACTTTATCCTTT AAAGCAGCAGCAATCACAAGGCACATATGTACAAAATACCTCAAGCAAAATAGAAACTGA ACATTCAAAAAGTATGTGTTGTTTAAAAAGATGTTGAAGACCATTTGAGTAATAAGCACA GGTTATAAAAATCACAATTTTATTCTTCTACCTTTAACAAGAGAAGGAATGATGGCATAA TATTTATATGCTATATTGTGGCACGTATAACAAACCTTTACACCACATAACCTGGTTTGC TAAAGAAAAAACAGGTAAAGTACTGCTTAAAAATGGTATTTTTAGTGCTTTCACATCTTT TGTAGATATCTGGAATTTGTAAGATGAAAAGAAAAATTCTCTTTTTCCTTGGCTTAGTAA AAGCCTGTAGTTACACGTCCCTAGGCAAACCCACCATACAAAAAAGGAATACATTTGAGA TATTCTAAAATATAAAGGATATGATTTATATTATGGGATCAACATAATAACCAACTCTCT TCCTAAAACAGATTAGAGGTGAACACATAAGAAATGTAAAGAAAGGTTAATTACATTAAA ATAAAATAGCTTCTGATTTAAATTATTGCTCACTTAGGAAGTATAATGAATATTCTCTGG CTAAGGATGCTCAGATTAGAAATGGTGTGACATCAATATTTAGTGTGTACAAAAAGTCTA AGATTAAATACATTGCANAGTTATAAATAATCACACAAAAGTGTCCTTTTAGCCACCATT TAGCTCACACAACGAAGACTGGAAAATACGTGGTTATTTAGATCTTCATATAAAATTGTA CACATATGTTGAAGTGTCCTCCTAGCTTTATGTTGCAGTCATTTTCTAATGAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006415 |
| Insert Size | 3010 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006415.2, NP_006406.1 |
| RefSeq Size | 2780 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 10558 |
| UniProt ID | O15269 |
| Domains | aminotran_1_2 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (1) represents the longest transcript, and encodes isoform a. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Expression of the ORMDLS, Modulators of Serine Palmitoyltransferase, is Regulated by Sphingolipids in Mammalian Cells
,Gupta, SD;Gable, K;Alexaki, A;Chandris, P;Proia, RL;Dunn, TM;Harmon, JM;,
J. Biol. Chem.
,PubMed ID 25395622
[SPTLC1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222985 | SPTLC1 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1 |
CNY 3,656.00 |
|
| RC222985L1 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222985L2 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, mGFP tagged |
CNY 6,080.00 |
|
| RC222985L3 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222985L4 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG222985 | SPTLC1 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1 |
CNY 5,256.00 |
