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GFM1 (NM_024996) Human Untagged Clone
CAT＃: SC111592
GFM1 (untagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein
CNY 5,512.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1; mtEF-G1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111592 sequence for NM_024996 edited (data generated by NextGen Sequencing)
ATGAGACTCCTGGGAGCTGCAGCCGTCGCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCC CTAGGCTGGCAGAGGAAGCAGGTTAATTGGAAGGCCTGCCGATGGTCTTCATCAGGGGTG ATTCCTAATGAAAAAATACGAAATATTGGAATCTCAGCTCACATTGATTCTGGGAAAACT ACATTAACAGAACGAGTCCTTTACTACACTGGCAGAATTGCAAAGATGCATGAGGTGAAA GGTAAAGATGGAGTTGGTGCTGTCATGGATTCCATGGAACTAGAGAGACAAAGAGGAATC ACTATTCAGTCAGCAGCCACTTACACCATGTGGAAAGATGTCAATATTAACATTATAGAT ACTCCTGGGCATGTGGACTTCACAATAGAAGTGGAAAGGGCCCTGAGAGTGTTGGATGGT GCAGTCCTTGTTCTCTGTGCTGTTGGAGGGGTACAGTGCCAGACCATGACTGTCAATCGT CAGATGAAGCGCTACAACGTTCCGTTTCTAACTTTTATTAACAAATTGGACCGAATGGGC TCCAACCCAGCCAGGGCCCTGCAGCAAATGAGGTCTAAACTAAATCATAATGCAGCGTTT ATGCAGATACCCATGGGTTTGGAGGGTAATTTTAAAGGTATTGTAGATCTTATTGAGGAA CGAGCCATCTATTTTGATGGAGACTTTGGTCAGATTGTTCGATATGGTGAGATTCCAGCT GAATTAAGGGCGGCGGCCACTGACCACCGGCAGGAGCTAATTGAATGTGTTGCCAATTCA GATGAACAGCTTGGTGAGATGTTTCTGGAAGNNAAAATCCCCTCGATTTCTGATTTAAAG CTAGCAATTCGAAGAGCTACTCTGAAAAGATCATTTACTCCTGTATTTTTGGGAAGCGCC TTGAAGAACAAAGGAGTTCAGCCTCTTTTAGATGCTGTTTTAGAATACCTCCCAAATCCA TCTGAAGTCCAGAACTATGCTATTCTCAATAAAGAGGATGACTCAAAAGAGAAAACCAAA ATCCTAATGAACTCCAGTAGAGACAATTCCCACCCATTTGTAGGCCTGGCTTTTAAACTG GAGGTAGGTCGATTTGGACAATTAACTTATGTTCGCAGTTATCAGGGAGAGCTAAAGAAG GGTGACACCATCTATAACACAAGGACAAGAAAGAAAGTACGGTTGCAACGGCTGGCTCGC ATGCATGCCGACATGATGGAGGATGTTGAGGAAGTATATGCCGGAGACATCTGTGCATTG TTTGGCATTGACTGTGCTAGTGGAGACACATTCACAGACAAAGCCAACAGCGGCCTTTCT ATGGAGTCAATTCATGTTCCTGATCCTGTCATTTCAATAGCAATGAAGCCTTCTAACAAG AACGATCTGGAAAAATTTTCAAAAGGTATTGGCAGGTTTACAAGAGAAGATCCCACATTT AAAGTATACTTTGACACTGAGAACAAAGAGACAGTTATATCTGGAATGGGAGAATTACAC CTGGAAATCTATGCTCAGAGGCTGGAAAGAGAGTATGGCTGTCCTTGTATCACAGGAAAG CCAAAAGTTGCCTTTCGAGAGACCATTACTGCCCCTGTCCCGTTTGACTTTACACATAAA AAACAATCAGGTGGTGCAGGCCAGTATGGAAAAGTAATAGGTGTCCTGGAGCCTCTGGAC CCAGAGGACTACACTAAATTGGAATTTTCAGATGAAACATTCGGATCAAATATTCCAAAG CAGTTTGTGCCTGCTGTAGAAAAGGGGTTTTTAGATGCCTGCGAGAAGGGCCCTCTTTCT GGTCACAAGCTCTCTGGGCTCCGGTTTGTCCTGCAAGATGGAGCACACCACATGGTTGAT TCTAATGAAATCTCTTTCATCCGAGCAGGAGAAGGTGCTCTTAAACAAGCCTTGGCAAAT GCAACATTATGTATTCTTGAACCTATTATGGCTGTGGAAGTTGTAGCTCCAAATGAATTT CAGGGACAAGTAATTGCAGGAATTAACCGACGCCATGGGGTAATCACTGGGCAAGATGGA GTTGAGGACTATTTTACACTGTATGCAGATGTCCCTCTAAATGATATGTTTGGTTATTCC ACTGAACTTAGGTCATGCACAGAGGGAAAGGGAGAATACACAATGGAGTATAGCAGGTAT CAGCCATGTTTACCATCCACACAAGAAGACGTCATTAATAAGTATTTGGAAGCTACAGGT CAACTTCCTGTTAAAAAAGGAAAAGCCAAGAACTAA Clone variation with respect to NM_024996.5 812 a=>n;813 a=>n >OriGene 5' read for NM_024996 unedited
AGGCATATTTGTNAATACGCACCCACTATAGGGCGCGCCGCGAATTCGCACGAGGCGGCG CCACGGGACTTTGACGCGTGCTCTGCGCTTGCCATGAGACTCCTGGGAGCTGCAGCCGTC GCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCCCTAGGCTGGCAGAGGAAGCAGGTTAAT TGGAAGGCCTGCCGATGGTCTTCATCAGGGGTGATTCCTAATGAAAAAATACGAAATATG GGAATCTCAGCTCACATTGATTCTGGGAAAACTACATTAACAGAACGAGTCCTTTACTAC ACTGGCAGAATTGCAAAGATGCATGAGGTGAAAGGTAAAGATGGAGTTGGTGCTGTCATG GATTCCATGGAACTAGAGAGACAAAGAGGAATCACTATTCAGTCAGCAGCCACTTACACC ATGTGGAAAGATGTCAATATTAACATTATAGATACTCCTGGGCATGTGGACTTCACAATA GAAGTGGAAAGGGCCCTGAGAGTGTTGGATGGTGCAGTCCTTGTTCTCTGTGCTGTTGGA GGGGTACAGTGCCAGACCATGACTGTCAATCGTCAGATGAAGCGCTACAACGTTCCGTTT CTAACTTTTATTAACAAATTGGACCGAATGGGCTCCAACCCAGCCAGGGCCCTGCAGCAA ATGAGGTCTAAACTCAATCATAATGCAGCGTTTATGCAAATACCCATGGGTTTGGAGGGT AATTTTAAAGGTATTGTAAATCTTATTGAGGAACGAGCCATCTATTTTGATGGAGACCTT TGTCANATTGTTCGAATGGTGAGATCCACCTGAAATAGGGGCGCNGCCACTGACCACGGN CGGGGCTAATTGAATGGGTTGCCATTAAAAGACACCTTGTGAGATGTTCGGAA >OriGene 3' read for NM_024996 unedited
CTTTGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTCCAAATTAAACTAGCATTCATTAGGTTGATGAAACTAACTCTATAA ATTACATTTGATTTACCTGTCCAAAAACTTTAAGATGAACACAAACAAGAATAGTTTATG CAAGGGTCTACTTTTTACTTCACAATAAACCACAGGGGAAGTNAATACTTATACTCCTAA AACAGAGGCAGTTACAAATTTGCAGAAACCACAAACTGAAATAAACAGACTTTTTTAAAA CAGAGAGAAATTGGGCTAATATTTTAATATCCCAATGAAGAATCTGAAAACACTCTCTAT GAAGAAATACGGAATCCACTCTTCAACAGAAAAGCCTGCCCATTAATGACACTCCAAGAA GTTCATGATATATGCGATTCACTTTCAAGTCTCAGCAAACTAAAAATAGAGTNTTGAGAC TTAAACAGATAATGGACGGTGAAAGCAAATNTGACAAAACAAATATTATTTGATAAACAA CCCGTTAACAAGGAAGTANNATGAAAGTTGTGGTATCCTTTGCATTTTTCAGCTTTCTCT ATGTGAAACTCTTATGATCAAAATTNGAAAATGACTACATTCTAAGCTAAAAGTTTACTG AAATAGTCTCTTTCCCCTAAATATTAACATTTTCAGNATAATATTTTTAATTGGGGATTT TCATGACTTTAAACCAATCATTGAGTGGAAATGACTAGAATACCTTTGAATTAACAACCC TCTGAATTAACAAAAAGGCTTTTTTGAAGAAAAAAACCCGTTTCTGGGCTAAAATTTTTG TTTAACACCCGAATTTTTCCCTGGATCCCTCAAGGTTAAACCCCCCATTCATTTAGGTCG CCACTCCATAGCAAGTTATTTTTGCTTTCCTTTTAAAAGAAAGGCCCGTACTCCAATCTT TAAAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_024996 |
| Insert Size | 3110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024996.5, NP_079272.4 |
| RefSeq Size | 3468 bp |
| RefSeq ORF | 2256 bp |
| Locus ID | 85476 |
| UniProt ID | Q96RP9 |
| Domains | EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV |
| Gene Summary | Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208239 | GFM1 (Myc-DDK-tagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein |
CNY 5,504.00 |
|
| RC208239L1 | Lenti ORF clone of Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,904.00 |
|
| RC208239L2 | Lenti ORF clone of Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC208239L3 | Lenti ORF clone of Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208239L4 | Lenti ORF clone of Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG208239 | GFM1 (tGFP-tagged) - Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein |
CNY 7,104.00 |
|
| SC324018 | GFM1 (untagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein |
CNY 5,512.00 |
