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POLR3H (NM_138338) Human Untagged Clone
CAT＃: SC111545
POLR3H (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C25; RPC8; RPC22.9 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111545 sequence for NM_138338 edited (data generated by NextGen Sequencing)
ATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGG AAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTAC AACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTC CCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATCCATTC CTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCT CTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTC GACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTC TACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACG TCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCAAAGAAG GAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCCTTCTCTCCTGG TGGACCAGCAACTAG Clone variation with respect to NM_138338.3 >OriGene 5' read for NM_138338 unedited
GTCCGAATATGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCGGGGT CTCGCGGCGCGGGCGCGCACCCGNGACTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGT TTCCTCATGTTGTAGTAGCGGAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCA ACCTCACCGCCAGCCTGGGGGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTC TCCCTGCCCCCGGCCCTGGTGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGG CCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTG AGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCG TGTACAACGTGGGACTCTGCATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATG TATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATC CATTCCTAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACG TCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCA AGTTCGACGAAGCGGAGCAAGTGTGGGTGTGGGAGTACCAGACGGAGGAAGGAGCACACG ACCTCTACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGNTG ACACGTNCCCCACAGGNCCCAGCTCAGCAGATGCCACCACTTNCAGTGANGAGCTGCCAA AGAAAGAGCTNCGTACACGCTTTGTGGATCCATCAGTGAGCCAGGCCCTGGCCTTCTCTT CTGGTGGACCCAGCACTAGCCCTGGNGGCTGNAAGTGAACCCTACCACCTGCGGNAANGG TGTATGNCNGCATGGAAGACACGCAGCTGN >OriGene 3' read for NM_138338 unedited
GGGTTTTATTCTGTTTCAGGCTCCCTCTAAGTTTTTCTCCTGCCTGATCATTTCATTGGT GGCTGAAGGATTCTAGAGAACCTTTTGTTCTTGCAAGGAAAACAAGAATCCAAAACCAGT GACTGTTCTGTGAGTGATTGGTGTCTGTGCCGTTTGTTGTCAAGTCCAGGGTCCAGGAAG GGTCTTTTCAGTCAAGGTCAGTGTTTTCCCAACAGCCACAGCCACAGATGGATCCATCAC TGCAGTGAAGGAGGAGCAGGGCCTAGATGGTGGAGGAGCGGGGCAAGCTGGTGGGCACTC CTGGCCTTGCCTCACTGTCCAGCTCGAGACACTATCTCCTTAGCATCGGCCTCAGCTGCT GTTGTCTTCACAGCCGGCCATACCACCTTCCCGCAGGCTGGTAGGGTCCACTGTCCAGCC CCAGGGCTAGTTGCTGGTCCACCAGGAGAGAAGGCCCAGGCCTGGCTCACTGATGGATCC CACAAGCGTGTACGGAGCCTCCTTCTTTGGCAGCTCCTCACTGGAAGTGGTGGCATCTGC TGAGCTGGGCCCTGTGGGGGACGTGTCAACAAAGCTCTCGTCCACCACCCGGAAGCGGAT CTCCTCGCCGGTGTCCATGTAGAGGTCGTGTGCTCCTTCCTCCGTCTCGTACTCCCACAC CCACACCTGGCTCGCTTCGTCGAACTTGGCTGGCTGCTGCAGTGACTCTGGGGGGATGAG AATGTCATCGAAAGAGCCTAGAGAGACGTGCACTCCTTCTGGGCTGCAGCCTTTGATCCT TCCNATGAGGATCTCAATCTAGGAATGGATGAAACCCACGCCAGCGAAAATGGACTTTGG TGTGTGAGCGCCATCCCCAGGGATACATGGCCATCCTCAGTTTTGGGGAATCAAACAACA AATGCGCAGTCCACGTTTTACCGAACTTGGTGGGCACCTTTCTGGTCACCTCCTCGGCAT GGG |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_138338 |
| Insert Size | 1400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138338.2, NP_612211.1 |
| RefSeq Size | 4159 bp |
| RefSeq ORF | 615 bp |
| Locus ID | 171568 |
| UniProt ID | Q9Y535 |
| Domains | RNA_pol_Rpb7_N |
| Protein Families | Transcription Factors |
| Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 3, 5, and 6 encode the same isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220534 | POLR3H (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1 |
CNY 2,400.00 |
|
| RC220534L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220534L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG220534 | POLR3H (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 1 |
CNY 4,000.00 |
