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TPK1 (NM_022445) Human Untagged Clone
CAT＃: SC111236
TPK1 (untagged)-Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1
CN¥ 2,400.00
CN¥ 2,950.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HTPK1; PP20; THMD5 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_022445, the custom clone sequence may differ by one or more nucleotides
ATGGAGCATGCCTTTACCCCGTTGGAGCCCCTGCTTTCCACTGGGAATTTGAAGTACTGCCTTGTAATTC TTAATCAGCCTTTGGACAACTATTTTCGTCATCTTTGGAACAAAGCTCTTTTAAGAGCCTGTGCCGATGG AGGTGCCAACCGCTTATATGATATCACCGAAGGAGAGAGAGAAAGCTTTTTGCCTGAATTCATCAATGGA GACTTTGATTCTATTAGGCCTGAAGTCAGAGAATACTATGCTACTAAGGGATGTGAGCTCATTTCAACTC CTGATCAAGACCACACTGACTTTACTAAGTGCCTTAAAATGCTCCAAAAGAAGATAGAAGAAAAAGACTT AAAGGTTGATGTGATCGTGACACTGGGAGGCCTTGCTGGGCGTTTTGACCAGATTATGGCATCTGTGAAT ACCTTGTTCCAAGCGACTCACATCACTCCTTTTCCAATTATAATAATCCAAGAGGAATCGCTGATCTACC TGCTCCAACCAGGAAAGCACAGGTTGCATGTAGACACTGGAATGGAGGGTGATTGGTGTGGCCTTATTCC TGTTGGACAGCCTTGTATGCAGGTTACAACCACAGGCCTCAAGTGGAACCTCACAAATGATGTGCTTGCT TTTGGAACATTGGTCAGTACTTCCAATACCTACGACGGGTCTGGTGTTGTGACTGTGGAAACTGACCACC CACTCCTCTGGACCATGGCCATCAAAAGCTAA >OriGene 5' read for NM_022445 unedited
GTCAAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGNATCTCCA GTCTGTGGCTCCTACCAGCCATTGTAGGCCAATAATCCGTTATGGAGCATGCCTTTACCC CGTTGGAGCCCCTGCTTTCCACTGGGAATTTGAAGTACTGCCTTGTAATTCTTAATCAGC CTTTGGACAACTATTTTCGTCATCTTTGGAACAAAGCTCTTTTAAGAGCCTGTGCCGATG GAGGTGCCAACCGCTTATATGATATCACCGAAGGAGAGAGAGAAAGCTTTTTGCCTGAAT TCATCAATGGAGACTTTGATTCTATTAGGCCTGAAGTCAGAGAATACTATGCTACTAAGG GATGTGAGCTCATTTCAACTCCTGATCAAGACCACACTGACTTTACTAAGTGCCTTAAAA TGCTCCAAAAGAAGATAGAAGAAAAAGACTTAAAGGTTGATGTGATCGTGACACTGGGAG GCCTTGCTGGGCGTTTTGACCAGATTATGGCATCTGTGAATACCTTGTTCCAAGCGACTC ACATCACTCCTTTTCCAATTATAATAATCCAAGAGGAATCGCTGATCTACCTGCTCCAAC CAGGAAAGCACAGGTTGCATGTAGACACTGGAATGGAGGGTGATTGGTGTGGCCTTATTC CTGTTGGACAGCCTTGTATGCAGGTTACAACCACAGGCCTCAAGTGGAACCTCACAAATG ATGTGCTTGCTTTTGGAACATTGGTCAGTACTTCCAATACCTACGACGGGTCTGGTGTTG TGACTGTGGAAACTGACCACCCACTCCTCTGGACCATGGCCATCANAAGCTAACCTGTTG ACTGGCATCCATAAGTGTGCCTCTGCCTTATCTCATTNCTCACAGNTCATTGCTCNACAG AACGATNACCCTGGGTTNGCAGAATACTAACTCTCTAGGGGAACCCACTG >OriGene 3' read for NM_022445 unedited
GGGCAATCCAGCCATTTTTNNAANNGCGTCGTGAACGCGGCCGCATACTANAGCGAGTTT TTTTTTTTTTTTTTTGGAAAGTATCCATTTATTAACACTGGTAACATTTTGAGGGCACAC AGAACATGCATTCTTTGGTGGCAAAATTTTAAAATCACAGATATATTTCTTTTATATATT ATGAATATATTTCTAGTAAGGAATATATAACTAGTCTTCAGAAACTGAAGATGGAAACAT AGCTAAGCCTCAACTTCCGGTCAAGCAGAAAGTAATGAAAAGTGCCGAAAATGCCACAGA CACATGGGCCACTGCTCCTTAAATGCCACAATCACGCGGGCCACTGCTCCTTTCATTCTC TACAGACAACCTGAGTCTCACACTGTCATAGCACCAAGAAGAAAACAGTTACTTTCACTT TTCTTATAGCAACATAGGGATTTGTGTACATAAAACATGTTGGACAGGTTCTACTTCAAT CATGGTTTGTATGTCAACAGTTGGTTTCATAAAACAGAGAAAGGTGGAAAGAAGATAGAA GATGGTCTCTTTACCAAAGAAAGGTGAATACATTTCGATGCACCAGGTGTTTAGGCTAGT CAGTAACTGTGTGTATTATAGCCCTATGACTGCTAAGTGCAATATTTAAAATTCAGTGTG GGAAAGACCTGAGAAACCATTAGTCCAACCGCTTTCATTNTACAGCTAAGGAAAAAGGTT CCCGAAACTTAGGTGAATATCATACTGGGATGAAAACCCAAGCTTTGGAGTTAGGCTCCT TAGTTTGGAAGTTATGTTTCCCCATAAACAAACTAATTTCTTGTTAATATGAATCCCCTC TGGGAGAAACCAACCATTTTTAAGTCAAAGTGATTGTAAAAACAAATCAATTTTATGGTA AAGTTCCTTGGCCAACTTTTTTACTTAAGGGTGGGAAAAAGAATCAAACCGTTACCCCCC TTAAAACTTGGCCTTTTACTTTTGAAATTNGGGGTATAGGGGAATAAGGGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022445 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022445.2, NP_071890.2 |
| RefSeq Size | 2449 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 27010 |
| UniProt ID | Q9H3S4 |
| Domains | TPK_catalytic, TPK_B1_binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Thiamine metabolism |
| Gene Summary | The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209721 | TPK1 (Myc-DDK-tagged)-Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1 |
CN¥ 2,400.00 |
|
| RC209721L3 | Lenti ORF clone of Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1, Myc-DDK-tagged |
CN¥ 5,890.00 |
|
| RC209721L4 | Lenti ORF clone of Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1, mGFP tagged |
CN¥ 5,890.00 |
|
| RG209721 | TPK1 (tGFP-tagged) - Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1 |
CN¥ 4,000.00 |
