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MTHFD1L (NM_015440) Human Untagged Clone
CAT＃: SC111221
MTHFD1L (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein
CNY 8,064.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | dJ292B18.2; FTHFSDC1; MTC1THFS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015440, the custom clone sequence may differ by one or more nucleotides
ATGGGCACGCGTCTGCCGCTCGTCCTGCGCCAGCTCCGCCGCCCGCCCCAGCCCCCGGGCCCTCCGCGCC GCCTCCGTGTGCCCTGTCGCGCTAGCAGCGGCGGCGGCGGAGGCGGCGGCGGTGGCCGGGAGGGCCTGCT TGGACAGCGGCGGCCGCAGGATGGCCAGGCCCGGAGCAGCTGCAGCCCCGGCGGCCGAACGCCCGCGGCG CGGGACTCCATCGTCAGAGAAGTCATTCAGAATTCAAAAGAAGTTCTAAGTTTATTGCAAGAAAAAAACC CTGCCTTCAAGCCGGTTCTTGCAATTATCCAGGCAGGTGACGACAACTTGATGCAGGAAATCAACCAGAA TTTGGCTGAGGAGGCTGGTCTGAACATCACTCACATTTGCCTCCCTCCAGATAGCAGTGAAGCCGAGATT ATAGATGAAATCTTAAAGATCAATGAAGATACCAGAGTACATGGCCTTGCCCTTCAGATCTCTGAGAACT TGTTTAGCAACAAAGTCCTCAATGCCTTGAAACCAGAAAAAGATGTGGATGGAGTAACAGACATAAACCT GGGGAAGCTGGTGCGAGGGGATGCCCATGAATGTTTTGTTTCACCTGTTGCCAAAGCTGTAATTGAACTT CTTGAAAAATCAGGTGTCAACCTAGATGGAAAGAAGATTTTGGTAGTGGGGGCCCATGGGTCTTTGGAAG CTGCTCTACAATGCCTGTTCCAGAGAAAAGGGTCCATGACAATGAGCATCCAGTGGAAAACACGCCAGCT TCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGCCAGAAGAGATTCCCCTTACT TGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATGACTTCCTGTCAGGGAAGGTTGGGTGTGGCT CTCCAAGAATACATTTTGGTGGACTCATTGAGGAAGATGATGTGATTCTCCTTGCTGCAGCTCTGCGAAT TCAGAACATGGTCAGTAGTGGAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGGTGGAGACTTCACTGC TTGAAACTTCAGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGACAAACTCCAAAAGCTG TGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAAAGCAAAGCCAA AGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAATACGTCTTAGTTGCTGGGATC ACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCATCGGGCTTGTGCAGGCTCTGACCGCACACC TGAATGTCAACTCCTTTGCCTGCTTGAGGCAGCCTTCCCAAGGACCGACGTTTGGAGTGAAAGGAGGAGC CGCGGGTGGTGGATATGCCCAGGTCATCCCCATGGAGGAGTTCAACCTTCACTTGACTGGAGACATCCAC GCCATCACCGCTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGATTCTTCATGAAAACACGCAAA CAGATAAGGCTCTGTATAATCGGCTGGTTCCTTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCAGCT TGCTCGGCTAAAAAAACTGGGAATAAATAAGACTGATCCGAGCACACTGACAGAAGAGGAAGTGAGTAAA TTTGCCCGTCTCGACATCGACCCATCTACCATCACGTGGCAGAGAGTATTGGATACAAATGACCGATTTC TACGAAAAATAACCATCGGGCAGGGAAACACAGAGAAGGGCCATTACCGGCAGGCGCAGTTTGACATCGC AGTGGCCAGCGAGATCATGGCGGTGCTGGCCCTGACGGACAGCCTCGCAGACATGAAGGCACGGCTGGGA AGGATGGTGGTGGCCAGTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGGGGGTGACAGGTGCTT TGACAGTTTTGATGAAAGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGGGACACCTGTGTTCGT GCATGCGGGCCCTTTTGCTAACATTGCTCACGGCAACTCTTCAGTGTTGGCTGATAAAATTGCCCTGAAA CTGGTTGGTGAAGAAGGATTTGTAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAATTCT TCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGCTCT GAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAGAAC ATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGGTTC CCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCTTGC AAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTGGAC TTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGGTTC CAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCCTGA GGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAGACC CACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTGACG TCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACTGCC CACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGCTTGTTCTAA >OriGene 5' read for NM_015440 unedited
GGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGTCTTTGGAA GCTGCTCTACAATGCCTGTTCCAGAGAAAGGGTCCATGACAATGAGCATCCAGTGGAAAA CACGCCAGCTTCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGC CAGAAGAGATTCCCCTTACTTGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATG ACTTCCTGTCAGGGAAGGTTGGGTGTGGCTCTCCAAGAATACATTTTGGTGGACTCATTG AGGAAGATGATGTGATTCTCCTTGCTGCAGCTCTGCGAATTCAGAACATGGTCAGTAGTG GAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGGTGGAGACTTCACTGCTTGAAACTTC AGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGACAAACTCCAAAAGCTG TGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAAA GCAAAGCCAAAGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAAT ACGTCTTAGTTGCTGGGATCACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCA TCGGGCTTGTGCANGCTCTGACCGCACACCTGAATGTCAACTCCTTTGCCTGCTTGAGGC AGCCTTCCCAAGGACCGACGTTTGGAGTGAAAGGAGGAGCCGCGGGTGGTGGATATGNCC CAGTCATCCCCATGGGAGAGNTCACCCTTCACTTGACTGGAGACATNCACGCCATCACCG CTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGAT >OriGene 3' read for NM_015440 unedited
TTGTACATTATGNACCGCGCCGCATTCTGANGATCGGTTTTTTTTTTTTTTTTTTTTGGT TTTCTAGATCCTGTTTATTTTCAGTTCAAACCAAAGTAGAAAACTTGAGAGTGGAAAATG TTACCTTTTAGTTCACACTCCTAATCCCTTAGTCCCCATAAAATAAACATTCTAAAGTGT AAGCAGTAGAAATAATGGAAACTCCACAGAAACAGAAATAAATTAGTTTCTTTCAGTCTT GGTGGAGGTCCTTTTGCCGAACACCATACTCCACTGTGAACAGAATTCATCTTGAACGAA GAAGAAATCTTTGGCCTATTTCACCACGTCTCCAGCATTGCATAACAGACATTTTTCAAA TTCAGTTTCTTCTCCAACTGCAGCAAAAAGGCAAAGAGTAGTCTGTTTCAGGAGTCTGCA TCGGGTCCTGTGAGAGCCTTGTCCACTTAGAACAAGCCTTTAACTTGTTCTGTTTCGGTA TCAAGATCTATGTCATAAAAGCAGGGCCGGGTGGGCAGTCCTGGCATGGTGCTCATCGTT CCGACCAAAGGGTAAATGAACCCAGCGCCTATGCTGGCCCGGACGTCACTGATAGGTAAG ATGAAGTCCCTTGGCACACCTTTTTTGTCAGGTTGGTGAGATAGAGAAAGGTGGGTCTTT GCCATGCAGATGGGCAAATTTCCAAAACCCTGTTGAGTGTAACGATCTATTTTGGCTTGT GCCTCAGGAGAGAGTTCAATATCTTTGGCTCCATAGACAGCCTGAGCAATGGTCCTTATC TTGTCCACAATTGGNAACCTGAACATCATACAGGAACTGGNAATCGCTTCTTTTACTCGC AGCCTCTCTCACAGCCCGAGCCAAGTCCACCGATTCTTTTCCACCACGGACCAGTGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015440 |
| Insert Size | 2750 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015440.1, NP_056255.1 |
| RefSeq Size | 3487 bp |
| RefSeq ORF | 2202 bp |
| Locus ID | 25902 |
| UniProt ID | Q6UB35 |
| Domains | FTHFS |
| Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate |
| Gene Summary | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223034 | MTHFD1L (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 10,744.00 |
|
| RC223034L1 | Lenti-ORF clone of MTHFD1L (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 13,144.00 |
|
| RC223034L2 | Lenti-ORF clone of MTHFD1L (mGFP-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC223034L3 | Lenti-ORF clone of MTHFD1L (Myc-DDK-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 5,890.00 |
|
| RC223034L4 | Lenti-ORF clone of MTHFD1L (mGFP-tagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 13,144.00 |
|
| RG223034 | MTHFD1L (tGFP-tagged) - Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC318430 | MTHFD1L (untagged)-Human methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), nuclear gene encoding mitochondrial protein |
CNY 7,220.00 |
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