SEM1 (NM_006304) Human Untagged Clone

CAT#: SC111062

SHFM1 (untagged)-Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1)



  "NM_006304" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms C7orf76; DSS1; ECD; PSMD15; SHFD1; Shfdg1; SHFM1; SHSF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111062 sequence for NM_006304 edited (data generated by NextGen Sequencing)
ATGTCAGAGAAAAAGCAGCCGGTAGACTTAGGTCTGTTAGAGGAAGACGACGAGTTTGAA
GAGTTCCCTGCCGAAGACTGGGCTGGCTTAGATGAAGATGAAGATGCACATGTCTGGGAG
GATAATTGGGATGATGACAATGTAGAGGATGACTTCTCTAATCAGTTACGAGCTGAACTA
GAGAAACATGGTTATAAGATGGAGACTTCATAG

Clone variation with respect to NM_006304.1
>OriGene 5' read for NM_006304 unedited
TGGGGGTTCGNATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGG
AGGGTTCCAACTTTTCTGCTTATCTGGGAGGTGTTGGGCGCGGACAGTCGAGATGTCAGA
GAAAAAGCAGCCGGTAGACTTAGGTCTGTTAGAGGAAGACGACGAGTTTGAAGAGTTCCC
TGCCGAAGACTGGGCTGGCTTAGATGAAGATGAAGATGCACATGTCTGGGAGGATAATTG
GGATGATGACAATGTAGAGGATGACTTCTCTAATCAGTTACGAGCTGAACTAGAGAAACA
TGGTTATAAGATGGAGACTTCATAGCATCCAGAAGAAGTGTTGAAGTAACCTAAACTTGA
CCTGCTTAATACATTCTAGGGCAGAGAACCCAGGATGGGACACTAAAAAAATGTGTTTAT
TTCATTATCTGCTTGGATTTATTTGTGTTTTTGTAACACAAAAAATAAATGTTTTGATAT
AATCTTGGTTTGGTTCAGACCACACACACCACCCCCCCCCCCCCCCCCACCCACCCACCC
AAAAAAACTCGACTCTAGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCCCGGG
TGGCATCCCTGTGACCCCTCCCCAGTGCCTTTCCTGGCCCTGGAAGTTGCCACTCCAGTG
CCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGGGTCCTTC
TATATATTATGGGGTGGGGGGGGGGCGTTGGAGCCAGGGGCCATTTGGGAAAACACCCGC
ACGGCCCTCGGGGCCTTATTGGACCCAACCGGAACCGCCGGCCCATCCTGGCTCCTGCAT
TCCCCCCCCCCGGGTCCAAGCCATTTCCCTGCCCCACCT
>OriGene 3' read for NM_006304 unedited
ATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTCGGAACCAAACCAAAATTATTTCAAAACATTTATTTTTTGGGT
TACAAAAACACAAATAAATCCAAGCAGATAATGAAATAAACACATTTTTTTAGGGGCCCA
TCCTGGGTTCTCTGCCCTAAAATGTATTAAGCAGGTCAAGTTTAGGTTACTTCAACACTT
TTTTTGGATGCTATGAAGTCTCCATTTTATAACCATGTTTCTTTAGTTCAGCTCGAAACT
GATTAAAAAAGTCATCCTTTACATTGGCATCATCCCAATTATCCTCCCAAACATGGGCAT
TTTCATTTTCATCTAAGCCAGCCCAGTCTTCGGGAGGGAACTTTTAAAACTCGTCGTTTT
CCTTTAACAAACCTAAGTCTACCGGCTGGTTTTTTTTTGACATTTGGACTGGCCGCGCCC
AACACCTCCCAAATAAGCAAAAAAGTTGGAACCCTCCCTCGTGCCGAATTCGCGGCCGCC
CTATAGGGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAAA
CCTCCCACCGTACACGCCTACCGCCCATTTGGGTCAACGGGGCGGGGTTATTACCACATT
TTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGG
AAACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGTTGTACTGCCAAAAC
CGCATCACCATGGTAATAGCGATGACTAAACNTANATGTACTGCCAAGTAGAAAGTCCCG
TAGGTCATGTACTGGCATAAATGCAGCGGGCCATTTACCGCANTGACGTCAATAGGGGCG
ACTNGCATAAGAACCTGGATGC
Restriction Sites NotI-NotI     
ACCN NM_006304
Insert Size 520 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006304.1, NP_006295.1
RefSeq Size 509 bp
RefSeq ORF 213 bp
Locus ID 7979
UniProt ID P60896
Domains DSS1_SEM1
Protein Pathways Homologous recombination, Proteasome
Gene Summary The product of this gene has been localized within the split hand/split foot malformation locus SHFM1 at chromosome 7. It has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (5) differs in the 3' exon structure and the entire coding region, compared to variant 1. It encodes isoform 3, which has a completely different sequence, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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