NFH (NEFH) (NM_021076) Human Untagged Clone

CAT#: SC110961

NEFH (untagged)-Human neurofilament, heavy polypeptide (NEFH)



  "NM_021076" in other vectors (4)

CNY 7,112.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
NEFH mouse monoclonal antibody,clone OTI10A7
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CMT2CC; NFH
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110961 sequence for NM_021076 edited (data generated by NextGen Sequencing)
ATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCATGGC
GGCGGCAGCCTCCACTACGCGCTAGCCCGAAAGGGTGGCGCAGGCGGGACGCGCTCCGCC
GCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTCCGTGAGCTCCGTGTCCGCC
TCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCACCGACTCGCTGGACACGCTG
AGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTCACGCAGTGAGAAGGAGCAG
CTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAAGGTGCGGCAGCTGGAGGCG
CACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCAGCAGCAGGCGGGCCGCTCC
GCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCGCGGCGCGGTGCTGCGCCTG
GGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCTGCTCGAGGACATCGCGCAC
GTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGAGGCCGAGGCGGCGGCCCGC
GCGCTGGCGCGCTTCGCGCAGGAGGCCGAGGCGGCGCGCGTGGACCTGCAGAAGAAGGCG
CAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCACCAGGAAGAGGTGGGCGAG
CTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCAGGCGCAGATGCAGGCCGAGACGCGC
GACGCCCTGAAGTGCGACGTGACGTCGGCGCTGCGCGAGATTCGCGCGCAGCTTGAAGGC
CACGCGGTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGCTGGACCGACTG
TCGGAGGCAGCCAAGGTGAACACAGACGCTATGCGCTCAGCGCAGGAGGAGATAACTGAG
TACCGGCGTCAGCTGCAGGCCAGGACCACAGAGCTGGAGGCACTGAAAAGCACCAAGGAC
TCACTGGAGAGGCAGCGCTCTGAGCTGGAGGACCGTCATCAGGCCGACATTGCCTCCTAC
CAGGAAGCCATTCAGCAGCTGGACGCTGAGCTGAGGAACACCAAGTGGGAGATGGCCGCC
CAGCTGCGAGAATACCAGGACCTGCTCAATGTCAAGATGGCTCTGGATATAGAGATAGCC
GCTTACAGAAAACTCCTGGAAGGTGAAGAGTGTCGGATTGGCTTTGGCCCAATTCCTTTC
TCGCTTCCAGAAGGACTCCCCAAAATTCCCTCTGTGTCCACTCACATAAAGGTGAAAAGC
GAAGAGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAGGAACAG
ACAGAGGAGACCCAAGTGACTGAAGAAGTGACTGAAGAAGAGGAGAAAGAGGCCAAAGAG
GAGGAGGGCAAGGAGGAAGAAGGGGGTGAAGAAGAGGAGGCAGAAGGGGGAGAAGAAGAA
ACAAAGTCTCCCCCAGCAGAAGAGGCTGCATCCCCAGAGAAGGAAGCCAAGTCACCAGTA
AAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCC
CCAGCCGAAGTCAAGTCCCCTGAGAAGGCCAAGTCTCCAGCAAAGGAAGAGGCAAAGTCA
CCGCCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCTCCAGCTGAGGTCAAGTCC
CCCGAGAAGGCCAAGTCCCCAGCAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCT
CCAGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCACCGGCTGAGGCCAAGTCC
CCAGTGAAGGAAGAAGCAAAATCTCCAGCTGAGGTCAAGTCCCCGGAAAAGGCCAAGTCT
CCAACGAAGGAGGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGAGAAGGAAGAGGCC
AAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCC
AAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAA
GAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCCCCTGAG
AAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGACCCCCGAGAAGGCCAAGTCCCCAGTG
AAGGAAGAAGCCAAGTCCCCAGAGAAGGCCAAGTCCCCAGAGAAGGCCAAGACTCTTGAT
GTGAAGTCTCCAGAAGCCAAGACTCCAGCGAAGGAGGAAGCAAGGTCCCCTGCAGACAAA
TTCCCTGAAAAGGCCAAAAGCCCTGTCAAGGAGGAGGTCAAGTCCCCAGAGAAGGCGAAA
TCTCCCCTGAAGGAGGATGCCAAGGCCCCTGAGAAGGAGATCCCAAAAAAGGAAGAGGTG
AAGTCCCCAGTGAAGGAGGAGGAGAAGCCCCAGGAGGTGAAAGTCAAAGAGCCCCCAAAG
AAGGCAGAGGAAGAGAAAGCCCCTGCCACACCAAAAACAGAGGAGAAGAAGGACAGCAAG
AAAGAGGAGGCACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCT
GCTGTCGAAAAGCCCAAAGAATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGAAGATAAG
AAAAAAGTCCCCACCCCAGAGAAGGAGGCTCCTGCCAAGGTGGAGGTGAAGGAAGACGCT
AAACCCAAAGAAAAGACAGAGGTGGCCAAGAAGGAACCAGATGATGCCAAGGCCAAGGAA
CCCAGCAAACCAGCAGAGAAGAAGGAGGCAGCACCGGAGAAAAAAGACACCAAGGAGGAG
AAGGCCAAGAAGCCTGAGGAGAAACCCAAGACAGAGGCCAAAGCCAAGGAAGATGACAAG
ACCCTCTCAAAAGAGCCTAGCAAGCCTAAGGCAGAAAAGGCTGAAAAATCCTCCAGCACA
GACCAAAAAGACAGCAAGCCTCCAGAGAAGGCCACAGAAGACAAGGCCGCCAAGGGGAAG
TAA

Clone variation with respect to NM_021076.3
2232 t=>c;2784 a=>g
>OriGene 5' read for NM_021076 unedited
NGGTCATAATTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGCCGTCCCGGC
CTCGCGCACCTGCTCAGGCCATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCC
CGTTCGCGCCGCTGCATGGCGGCGGCAGCCTCCACTACGCGCTAGCCCGAAGGGTGGCGC
AGGCGGGACGCGCTCCGCCGCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTC
CGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCAC
CGACTCGCTGGACACGCTGAGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTC
ACGCAGTGAGAAGGAGCAGCTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAA
GGTGCGGCAGCTGGAGGCGCACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCA
GCAGCAGGCGGGCCGCTCCGCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCG
CGGCGCGGTGCTGCGCCTGGGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCT
GCTCGAGGACATCGCGCACGTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGA
GGCCGAGGCGGCGGCCCGCGCGCTGGCGCGCTTCGCGCANGAGGCCGAGGCGGCGCGCGT
GGACCTGCAGAAGAAGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCAC
CAGGAAGAGGTGGGCGAGCTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCCAGCGCAG
ATGCANGCCGAGACGCGCGACGCCCTGAAGTGCGACGTGACGTCCGCGCTGCGCGAGATC
C
>OriGene 3' read for NM_021076 unedited
GTACCGCGGCACGCAATCTAGTAGTCGAGNNNNNNTTTTTTTTTTTTTTTTTTTCCATTT
TATAAGCACTTGGTTTTATTGCACAGATACAATTGAAAGTGAACTCCAGTGGAAAGTGCG
CCCTGGCATAATTCATTGGCCAGTGCCCGGCCCCACCTGGACCGCTCTTCCAAAATAAAG
AGCTCTGGGCAAGGGCTTGACCACTCCTTTGGAAAACAGAAGGCATTTGGGAATAGGTTA
AGACATCTCAAGCATAATGTATTTCTAACTGCTCATTCATTATTGCGGTCATCTGTCAGT
TGCACATAGAGGCAATAAATGCACTTATTTGGAAAGGAGGGGGGCAGTTTTTATAGTCAA
GTGTTTACGTGTGGCATTCGGCATGTATCACATTCAGCTACATAAGCTATCATAATTGTC
CATCGCCTGGGGAGGGCATGGGGGCAGGGGCCTGGGAGTGCCCTCTCTTGCTAACATATG
GCTAACAAAAATTCCTGTTTGAAGAAGGAGGCCCCGTCATCTAACCAGTTTTCTTTTTAC
TTAAAAACAGAAAAAGTGAAAATTGTGTTACTCTGATCCTTGACCTCCGGGACTCTTCTG
AGTTTTTTGGCCTGTTCCGGTGTTCCTTTCCCCTGCCTTACTTCCCCTGGGCGGCCTTGT
TTTTTGGGGCCTTCTTTGAAGCTCGCTGTCTTTTTGGCCGGGCCTGAAGAATTTTAAACC
CTTTCTGCCCTAGCTTGCAAGGCTCTTTAAAAAGGCCTTCGGAATTTCTCTTGGCTTAGC
CCTCTGTCTTGGGTTCCTCCCAAAAGCTTTTGGACCCTTCCCCCCCTCGGTGTCTCTTTN
TCCCCGGGCCCGCCCCTTTCTCTCTGCCCGTTTCGCGTGGTCTCTCCGCCCGGTCATATC
TCGTTTTCTTTTTTCCCACCCCCGGTTTCCTTTGACTAAGCGCTTCTCCTATCCTCCACT
TTATGAGACTACTCTTTTGGCGGTCCACTCCTCTCTTCTTCCGCCCGTCCTCCTCGGTCC
CTGCCCGTCTCTCCTCG
Restriction Sites NotI-NotI     
ACCN NM_021076
Insert Size 3740 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021076.2, NP_066554.2
RefSeq Size 3695 bp
RefSeq ORF 3063 bp
Locus ID 4744
UniProt ID P12036
Domains filament
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS)
Gene Summary Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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