NFH (NEFH) (NM_021076) Human Untagged Clone
CAT#: SC110961
NEFH (untagged)-Human neurofilament, heavy polypeptide (NEFH)
CNY 7,112.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CMT2CC; NFH |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC110961 sequence for NM_021076 edited (data generated by NextGen Sequencing)
ATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCATGGC GGCGGCAGCCTCCACTACGCGCTAGCCCGAAAGGGTGGCGCAGGCGGGACGCGCTCCGCC GCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTCCGTGAGCTCCGTGTCCGCC TCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCACCGACTCGCTGGACACGCTG AGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTCACGCAGTGAGAAGGAGCAG CTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAAGGTGCGGCAGCTGGAGGCG CACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCAGCAGCAGGCGGGCCGCTCC GCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCGCGGCGCGGTGCTGCGCCTG GGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCTGCTCGAGGACATCGCGCAC GTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGAGGCCGAGGCGGCGGCCCGC GCGCTGGCGCGCTTCGCGCAGGAGGCCGAGGCGGCGCGCGTGGACCTGCAGAAGAAGGCG CAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCACCAGGAAGAGGTGGGCGAG CTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCAGGCGCAGATGCAGGCCGAGACGCGC GACGCCCTGAAGTGCGACGTGACGTCGGCGCTGCGCGAGATTCGCGCGCAGCTTGAAGGC CACGCGGTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGCTGGACCGACTG TCGGAGGCAGCCAAGGTGAACACAGACGCTATGCGCTCAGCGCAGGAGGAGATAACTGAG TACCGGCGTCAGCTGCAGGCCAGGACCACAGAGCTGGAGGCACTGAAAAGCACCAAGGAC TCACTGGAGAGGCAGCGCTCTGAGCTGGAGGACCGTCATCAGGCCGACATTGCCTCCTAC CAGGAAGCCATTCAGCAGCTGGACGCTGAGCTGAGGAACACCAAGTGGGAGATGGCCGCC CAGCTGCGAGAATACCAGGACCTGCTCAATGTCAAGATGGCTCTGGATATAGAGATAGCC GCTTACAGAAAACTCCTGGAAGGTGAAGAGTGTCGGATTGGCTTTGGCCCAATTCCTTTC TCGCTTCCAGAAGGACTCCCCAAAATTCCCTCTGTGTCCACTCACATAAAGGTGAAAAGC GAAGAGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAGGAACAG ACAGAGGAGACCCAAGTGACTGAAGAAGTGACTGAAGAAGAGGAGAAAGAGGCCAAAGAG GAGGAGGGCAAGGAGGAAGAAGGGGGTGAAGAAGAGGAGGCAGAAGGGGGAGAAGAAGAA ACAAAGTCTCCCCCAGCAGAAGAGGCTGCATCCCCAGAGAAGGAAGCCAAGTCACCAGTA AAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCC CCAGCCGAAGTCAAGTCCCCTGAGAAGGCCAAGTCTCCAGCAAAGGAAGAGGCAAAGTCA CCGCCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCTCCAGCTGAGGTCAAGTCC CCCGAGAAGGCCAAGTCCCCAGCAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCT CCAGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCACCGGCTGAGGCCAAGTCC CCAGTGAAGGAAGAAGCAAAATCTCCAGCTGAGGTCAAGTCCCCGGAAAAGGCCAAGTCT CCAACGAAGGAGGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGAGAAGGAAGAGGCC AAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCC AAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAA GAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCCCCTGAG AAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGACCCCCGAGAAGGCCAAGTCCCCAGTG AAGGAAGAAGCCAAGTCCCCAGAGAAGGCCAAGTCCCCAGAGAAGGCCAAGACTCTTGAT GTGAAGTCTCCAGAAGCCAAGACTCCAGCGAAGGAGGAAGCAAGGTCCCCTGCAGACAAA TTCCCTGAAAAGGCCAAAAGCCCTGTCAAGGAGGAGGTCAAGTCCCCAGAGAAGGCGAAA TCTCCCCTGAAGGAGGATGCCAAGGCCCCTGAGAAGGAGATCCCAAAAAAGGAAGAGGTG AAGTCCCCAGTGAAGGAGGAGGAGAAGCCCCAGGAGGTGAAAGTCAAAGAGCCCCCAAAG AAGGCAGAGGAAGAGAAAGCCCCTGCCACACCAAAAACAGAGGAGAAGAAGGACAGCAAG AAAGAGGAGGCACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCT GCTGTCGAAAAGCCCAAAGAATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGAAGATAAG AAAAAAGTCCCCACCCCAGAGAAGGAGGCTCCTGCCAAGGTGGAGGTGAAGGAAGACGCT AAACCCAAAGAAAAGACAGAGGTGGCCAAGAAGGAACCAGATGATGCCAAGGCCAAGGAA CCCAGCAAACCAGCAGAGAAGAAGGAGGCAGCACCGGAGAAAAAAGACACCAAGGAGGAG AAGGCCAAGAAGCCTGAGGAGAAACCCAAGACAGAGGCCAAAGCCAAGGAAGATGACAAG ACCCTCTCAAAAGAGCCTAGCAAGCCTAAGGCAGAAAAGGCTGAAAAATCCTCCAGCACA GACCAAAAAGACAGCAAGCCTCCAGAGAAGGCCACAGAAGACAAGGCCGCCAAGGGGAAG TAA Clone variation with respect to NM_021076.3 2232 t=>c;2784 a=>g >OriGene 5' read for NM_021076 unedited
NGGTCATAATTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGCCGTCCCGGC CTCGCGCACCTGCTCAGGCCATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCC CGTTCGCGCCGCTGCATGGCGGCGGCAGCCTCCACTACGCGCTAGCCCGAAGGGTGGCGC AGGCGGGACGCGCTCCGCCGCTGGCTCCTCCAGCGGCTTCCACTCGTGGACACGGACGTC CGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGTGGCGCAGGCGCCGCCTCAAGCAC CGACTCGCTGGACACGCTGAGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCTC ACGCAGTGAGAAGGAGCAGCTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAA GGTGCGGCAGCTGGAGGCGCACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCA GCAGCAGGCGGGCCGCTCCGCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCG CGGCGCGGTGCTGCGCCTGGGCGCGGCGCGCGGTCAGCTACGCCTGGAGCAGGAGCACCT GCTCGAGGACATCGCGCACGTGCGCCAGCGCCTAGACGACGAGGCCCGGCAGCGAGAGGA GGCCGAGGCGGCGGCCCGCGCGCTGGCGCGCTTCGCGCANGAGGCCGAGGCGGCGCGCGT GGACCTGCAGAAGAAGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCACCAC CAGGAAGAGGTGGGCGAGCTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCCAGCGCAG ATGCANGCCGAGACGCGCGACGCCCTGAAGTGCGACGTGACGTCCGCGCTGCGCGAGATC C >OriGene 3' read for NM_021076 unedited
GTACCGCGGCACGCAATCTAGTAGTCGAGNNNNNNTTTTTTTTTTTTTTTTTTTCCATTT TATAAGCACTTGGTTTTATTGCACAGATACAATTGAAAGTGAACTCCAGTGGAAAGTGCG CCCTGGCATAATTCATTGGCCAGTGCCCGGCCCCACCTGGACCGCTCTTCCAAAATAAAG AGCTCTGGGCAAGGGCTTGACCACTCCTTTGGAAAACAGAAGGCATTTGGGAATAGGTTA AGACATCTCAAGCATAATGTATTTCTAACTGCTCATTCATTATTGCGGTCATCTGTCAGT TGCACATAGAGGCAATAAATGCACTTATTTGGAAAGGAGGGGGGCAGTTTTTATAGTCAA GTGTTTACGTGTGGCATTCGGCATGTATCACATTCAGCTACATAAGCTATCATAATTGTC CATCGCCTGGGGAGGGCATGGGGGCAGGGGCCTGGGAGTGCCCTCTCTTGCTAACATATG GCTAACAAAAATTCCTGTTTGAAGAAGGAGGCCCCGTCATCTAACCAGTTTTCTTTTTAC TTAAAAACAGAAAAAGTGAAAATTGTGTTACTCTGATCCTTGACCTCCGGGACTCTTCTG AGTTTTTTGGCCTGTTCCGGTGTTCCTTTCCCCTGCCTTACTTCCCCTGGGCGGCCTTGT TTTTTGGGGCCTTCTTTGAAGCTCGCTGTCTTTTTGGCCGGGCCTGAAGAATTTTAAACC CTTTCTGCCCTAGCTTGCAAGGCTCTTTAAAAAGGCCTTCGGAATTTCTCTTGGCTTAGC CCTCTGTCTTGGGTTCCTCCCAAAAGCTTTTGGACCCTTCCCCCCCTCGGTGTCTCTTTN TCCCCGGGCCCGCCCCTTTCTCTCTGCCCGTTTCGCGTGGTCTCTCCGCCCGGTCATATC TCGTTTTCTTTTTTCCCACCCCCGGTTTCCTTTGACTAAGCGCTTCTCCTATCCTCCACT TTATGAGACTACTCTTTTGGCGGTCCACTCCTCTCTTCTTCCGCCCGTCCTCCTCGGTCC CTGCCCGTCTCTCCTCG |
Restriction Sites | NotI-NotI |
ACCN | NM_021076 |
Insert Size | 3740 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_021076.2, NP_066554.2 |
RefSeq Size | 3695 bp |
RefSeq ORF | 3063 bp |
Locus ID | 4744 |
UniProt ID | P12036 |
Domains | filament |
Protein Families | Druggable Genome |
Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
Gene Summary | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213487 | NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH) |
CNY 7,104.00 |
|
RC213487L3 | Lenti-ORF clone of NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH) |
CNY 9,504.00 |
|
RC213487L4 | Lenti-ORF clone of NEFH (mGFP-tagged)-Human neurofilament, heavy polypeptide (NEFH) |
CNY 12,070.00 |
|
RG213487 | NEFH (tGFP-tagged) - Human neurofilament, heavy polypeptide (NEFH) |
CNY 8,704.00 |