NEDD8 (NM_006156) Human Untagged Clone

CAT#: SC110960

NEDD8 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 8 (NEDD8)



  "NM_006156" in other vectors (6)

CNY 1,200.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-NEDD8 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NEDD-8
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_006156 edited
GAATTCGGCACGAGGGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCGGCGACCGGA
GCGGTAGGAGCAGCAATTTATCCGTGTGCAGCCCCAAACTGGAAAGAAGATGCTAATTAA
AGTGAAGACGCTGACCGGAAAGGAGATTGAGATTGACATTGAACCTACAGACAAGGTGGA
GCGAATCAAGGAGCGTGTGGAGGAGAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCAT
CTACAGTGGCAAGCAGATGAATGATGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGG
TTCAGTCCTTCACCTGGTGTTGGCTCTGAGAGGAGGAGGTGGTCTTAGGCAGTGATGGAC
CCTCCATTTTACCTCTTTACCCTGTCGCTCATAATGAGGCATCATATATCCTCTCACTCT
CTGGGACACCATAGCCACTGCCCCCTCCCCTGGATGCCCAGTAATGTATGTCTACTGGTG
GGAGACTGTGAGGATCCCAGGATTCAGTATTCCTGGCCCAGAGGGCCCTTGCTGGCTACT
GGGTGTTAGTTTGCAGTCCTGTGTGCTTCCCTCTCTTATGACTGTGTCCCTGGTTGTCAA
TAAAATATTTCCTGGCCTCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_006156 unedited
GGTTGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCCCTTG
CAGCAAGATGCTGGAGGGCGGCAGCGGCGACCGGAGCGGTAGGAGCAGCAATTTATCCGT
GTGCAGCCCCAANACTGGAAAGAAGATGCTAATTAAAGTGAAGACGCTGACCGGAAAGGA
GATTGAGATTGACATTGAACCTACAGACAAGGTGGAGCGAATCAAGGAGCGTGTGGAGGA
GAAAGAGGGAATCCCCCCACAACAGCAGAGGCTCATCTACAGTGGCAAGCAGATGAATGA
TGAGAAGACAGCAGCTGATTACAAGATTTTAGGTGGTTCAGTCCTTCACCTGGTGTTGGC
TCTGAGAGGAGGAGGTGGTCTTAGGCAGTGATGGACCCTCCATTTTACCTCTTTACCCTG
TCGCTCATAATGAGGCATCATATATCCTCTCACTCTCTGGGACACCATAGCCACTGCCCC
CTCCCCTGGATGCCCAGTAATGTATGTCTACTGGTGGGAGACTGTGAGGATCCCAGGATT
CAGTATTCCTGGCCCAGAGGGCCCTTGCTGGCTACTGGGTGTTAGTTTGCAGTCCTGTGT
GCTTCCCTCTCTTATGACTGTGTCCCTGGTTGTCAATAAAATATTTCCTGGCCTCCTGGA
AAAAAAAAAAANNNNNAAAANNNNAAAAAAAAANAAAAAANAAAAAAAANAAAAAAAAAN
AAAAAAAANNAAAAAAAANNAANAAAAAAAAAAAAANAAAAAAAAANANNANAAAACAAA
AAAAAAAAANNCCTCTCCTTTTTTTTTGTGGGCGGGGTTAAATTTTGTTTTTAAAAAAAA
CCCGGGGGGCTTCTTTTGACCCCCCCCCAAGGTTTTTTTTGGGGCGGGAAGGTCCCCTCC
GGGGCGCCCCATTTTGTTTATAAAN
>OriGene 3' read for NM_006156 unedited
TATCTTATTTTTAACCAGGGNACACAGCTCATAAGAATAGGGGAAGCACACAGGACTGCA
AACTAACACCCAGTAGCCAGCAAGGGCCCTCTGGGCCAGGAATACTGAATCCTGGGATCC
TCACAGTCTCCCACCAGTAGACATACATTACTGGGCATCCAGGGGAGGGGGCAGTGGCTA
TGGTGTCCCAGAGAGTGAGAGGATATATGATGCCTCATTATGAGCGACAGGGTAAAGAGG
TGAAATGGAGGGTCCATCACTGCCTAAGACCACCTCCTCCTCTCAGAGCCAACACCAGGT
GAAGGACTGAACCACCTAAAATCTTGTAATCAGCTGCTGTCTTCTCATCATTCATCTGCT
TGCCACTGTAGATGAGCCTCTGCTGTTGTGGGGGGATTCCCTCTTTCTCCTCCACACGCT
CCTTGATTCGCTCCACCTTGTCTGTAGGTTCAATGTCCATCTCAATCTCCTTTCCGGTCA
GCGTCTTCACTTTAATTACCATCCTCTTTCCAGTTTGGGGCTGCACACGGATAAATTGCT
GCTCCTACCGCTCCGGTCGCCGCTGCCGCCCTCAGCACTCTTGGTGCATAGGCCCCTCAG
CCGAATTCCCGGCCGCCTATAATGAGCGTATTAAAAATTCTGACGGGTCCCTAACCGAGC
CTGCTTATATAGACCCTCCACCGTACCACGCTACCGCCCATTTGCGTAACGGGGCGGGGC
TATACCACCTTTTGGAAATTCCCGTGATTTTGGGCCCAACAAATTTCCTTTGCTCCAGGG
GGGGGAACTTTGGAATCCCTGGGTACAACCCTCTCCCCCCCACTGGGGTTTCGCATACCC
TTTCCCCTGGGATTCCGTGATCATCCCTATATTCCCCCTCACCAAATCCTTTATGTATTC
CGGCTCCCCCCTTGGGTCCTCCCCGCTGGACTAAAGGGGGGCCTCCTTTAACCACCTGTT
TTCCCGGCGTTTCGTACATCCCCTTGGTATGAAACCTCTGGCCATGGCATAN
Restriction Sites NotI-NotI     
ACCN NM_006156
Insert Size 1870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006156.1, NP_006147.1
RefSeq Size 605 bp
RefSeq ORF 246 bp
Locus ID 4738
UniProt ID Q15843
Protein Families Druggable Genome
Gene Summary Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...