hemoglobin subunit gamma 1 (HBG1) (NM_000559) Human Untagged Clone
CAT#: SC110916
HBG1 (untagged)-Human hemoglobin, gamma A (HBG1)
CNY 1,200.00
CNY 2,950.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HBG-T2; HBGA; HBGR; HSGGL1; PRO2979 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_000559, the custom clone sequence may differ by one or more nucleotides
ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATG CTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGG CAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACT TCCTTGGGAGATGCCACAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACT GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAAT CCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGT GCCCTGTCCTCCAGATACCACTGA >OriGene 5' read for NM_000559 unedited
GGGTCCACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGAACGTCT GAGGTTATCAATAAGCTCCTAGTCCAGACGCCATGGGTCATTTCACAGAGGAGGACAAGG CTACTATCACAAGCCTGTGGGGCAAGGTGAATGTGGAAGATGCTGGAGGAGAAACCCTGG GAAGGCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGT CCTCTGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGA CTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGA GTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATG TGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTT CCTGGCAGAAGATGGTGACTGGAGTGGCCAGTGCCCTGTCCTCCAGATACCACTGAGCTC ACTGCCCATGATGCAGAGCTTTCAAGGATAGGCTTTATTCTGCAAGCAATCAAATAATAA ATCTATTCTGCTAAGAGATCAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC TCTCCTGGCCCTGGAAAGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAG TTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATATGGGGTGAGGGGGTGGGGTT TGGACCAGGGGCCAATTGGAAAACAACCCGAAGGCCCCCGGGTCTATGGGAAC >OriGene 3' read for NM_000559 unedited
NNAAGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGATCT CTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAAGCTC TGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACTCCAGTCA CCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATTGCCA AAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGCTTGT CACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGG CATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGA TGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGGTAGA CAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCCCACA GGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCTGGACTAGGAG CTTATTGATAACCTCAGACGTTCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCG TATTACAAAAATCTGACGGTTCACCTAACGAGCTCTGCTTATATAGACCTCCCACCGTAC ACGCCTACCGGCCATTTGGGGCAACGGGGCGGGGTTATACCACATTTTGGAAAGTCCCCG TGATTTTGGGGCCAAAACAACTCCCATTGACGCAATGGGGCGGAGCTTTGGAAATCCCCG GAGGCAAACCCGCTCTCCCGCCCTGGGCGGACCGCCCAACCCCCCTCCCCTGGTAAAAGC AGGCCTATACCGCATGGCCGCCCACCGCAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_000559 |
Insert Size | 444 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_000559.2, NP_000550.2 |
RefSeq Size | 584 bp |
RefSeq ORF | 444 bp |
Locus ID | 3047 |
UniProt ID | P69891 |
Domains | globin |
Gene Summary | The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism
,Derek M. Dykxhoorn, Lisa D. Schlehuber, Irving M. London, and Judy Lieberman,
Proc Natl Acad Sci USA 2006 Apr 11;103(15):5953-8.
[HBG1]
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Other Versions
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RC203084 | HBG1 (Myc-DDK-tagged)-Human hemoglobin, gamma A (HBG1) |
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RC203084L1 | Lenti ORF clone of Human hemoglobin, gamma A (HBG1), Myc-DDK-tagged |
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RC203084L2 | Lenti ORF clone of Human hemoglobin, gamma A (HBG1), mGFP tagged |
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RC203084L3 | Lenti ORF clone of Human hemoglobin, gamma A (HBG1), Myc-DDK-tagged |
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RC203084L4 | Lenti ORF clone of Human hemoglobin, gamma A (HBG1), mGFP tagged |
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RG203084 | HBG1 (tGFP-tagged) - Human hemoglobin, gamma A (HBG1) |
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