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HIF 2 alpha (EPAS1) (NM_001430) Human Untagged Clone
CAT＃: SC110883
EPAS1 (untagged)-Human endothelial PAS domain protein 1 (EPAS1)
CNY 9,568.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001430 edited
CCCGCCCGGGCCGCGGGGAGCGGACGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAG GTGCTCGGCGTCTGAACGTCTCAAAGGGCCACAGCGACAATGACAGCTGACAAGGAGAAG AAAAGGAGTAGCTCGGAGAGGAGGAAGGAGAAGTCCCGGGATGCTGCGCGGTGCCGGCGG AGCAAGGAGACGGAGGTGTTCTATGAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTG AGCTCCCATCTGGACAAGGCCTCCATCATGCGACTGGCAATCAGCTTCCTGCGAACACAC AAGCTCCTCTCCTCAGTTTGCTCTGAAAACGAGTCCGAAGCCGAAGCTGACCAGCAGATG GATAACTTGTACCTGAAAGCCTTGGAGGGTTTCATTGCCGTGGTGACCCAAGATGGCGAC ATGATCTTTCTGTCAGAAAACATCAGCAAGTTCATGGGACTTACACAGGTGGAGCTAACA GGACATAGTATCTTTGACTTCACTCATCCCTGCGACCATGAGGAGATTCGTGAGAACCTG AGTCTCAAAAATGGCTCTGGTTTTGGGAAAAAAAGCAAAGACATGTCCACAGAGCGGGAC TTCTTCATGAGGATGAAGTGCACGGTCACCAACAGAGGCCGTACTGTCAACCTCAAGTCA GCCACCTGGAAGGTCTTGCACTGCACGGGCCAGGTGAAAGTCTACAACAACTGCCCTCCT CACAATAGTCTGTGTGGCTACAAGGAGCCCCTGCTGTCCTGCCTCATCATCATGTGTGAA CCAATCCAGCACCCATCCCACATGGACATCCCCCTGGATAGCAAGACCTTCCTGAGCCGC CACAGCATGGACATGAAGTTCACCTACTGTGATGACAGAATCACAGAACTGATTGGTTAC CACCCTGAGGAGCTGCTTGGCCGCTCAGCCTATGAATTCTACCATGCGCTAGACTCCGAG AACATGACCAAGAGTCACCAGAACTTGTGCACCAAGGGTCAGGTAGTAAGTGGCCAGTAC CGGATGCTCGCAAAGCATGGGGGCTACGTGTGGCTGGAGACCCAGGGGACGGTCATCTAC AACCCTCGCAACCTGCAGCCCCAGTGCATCATGTGTGTCAACTACGTCCTGAGTGAGATT GAGAAGAATGACGTGGTGTTCTCCATGGACCAGACTGAATCCCTGTTCAAGCCCCACCTG ATGGCCATGAACAGCATCTTTGATAGCAGTGGCAAGGGGGCTGTGTCTGAGAAGAGTAAC TTCCTATTCACCAAGCTAAAGGAGGAGCCCGAGGAGCTGGCCCAGCTGGCTCCCACCCCA GGAGACGCCATCATCTCTCTGGATTTCGGGAATCAGAACTTCGAGGAGTCCTCAGCCTAT GGCAAGGCCATCCTGCCCCCGAGCCAGCCATGGGCCACGGAGTTGAGGAGCCACAGCACC CAGAGCGAGGCTGGGAGCCTGCCTGCCTTCACCGTGCCCCAGGCAGCTGCCCCGGGCAGC ACCACCCCCAGTGCCACCAGCAGCAGCAGCAGCTGCTCCACGCCCAATAGCCCTGAAGAC TATTACACATCTTTGGATAACGACCTGAAGATTGAAGTGATTGAGAAGCTCTTCGCCATG GACACAGAGGCCAAGGACCAATGCAGTACCCAGACGGATTTCAATGAGCTGGACTTGGAG ACACTGGCACCCTATATCCCCATGGACGGGGAAGACTTCCAGCTAAGCCCCATCTGCCCC GAGGAGCGGCTCTTGGCGGAGAACCCACAGTCCACCCCCCAGCACTGCTTCAGTGCCATG ACAAACATCTTCCAGCCACTGGCCCCTGTAGCCCCGCACAGTCCCTTCCTCCTGGACAAG TTTCAGCAGCAGCTGGAGAGCAAGAAGACAGAGCCCGAGCACCGGCCCATGTCCTCCATC TTCTTTGATGCCGGAAGCAAAGCATCCCTGCCACCGTGCTGTGGCCAGGCCAGCACCCCT CTCTCTTCCATGGGGGGCAGATCCAATACCCAGTGGCCCCCAGATCCACCATTACATTTT GGGCCCACAAAGTGGGCCGTCGGGGATCAGCGCACAGAGTTCTTGGGAGCAGCGCCGTTG GGGCCCCCTGTCTCTCCACCCCATGTCTCCACCTTCAAGACAAGGTCTGCAAAGGGTTTT GGGGCTCGAGGCCCAGACGTGCTGAGTCCGGCCATGGTAGCCCTCTCCAACAAGCTGAAG CTGAAGCGACAGCTGGAGTATGAAGAGCAAGCCTTCCAGGACCTGAGCGGGGGGGACCCA CCTGGTGGCAGCACCTCACATTTGATGTGGAAACGGATGAAGAACCTCAGGGGTGGGAGC TGCCCTTTGATGCCGGACAAGCCACTGAGCGCAAATGTACCCAATGATAAGTTCACCCAA AACCCCATGAGGGGCCTGGGCCATCCCCTGAGACATCTGCCGCTGCCACAGCCTCCATCT GCCATCAGTCCCGGGGAGAACAGCAAGAGCAGGTTCCCCCCACAGTGCTACGCCACCCAG TACCAGGACTACAGCCTGTCGTCAGCCCACAAGGTGTCAGGCATGGCAAGCCGGCTGCTC GGGCCCTCATTTGAGTCCTACCTGCTGCCCGAACTGACCAGATATGACTGTGAGGTGAAC GTGCCCGTGCTGGGAAGCTCCACGCTCCTGCAAGGAGGGGACCTCCTCAGAGCCCTGGAC CAGGCCACCTGAGCCAGGCCTTCTACCTGGGCAGCACCTCTGCCGACGCCGTCCCACCAG CTTCACTCTCTCCGTCTGTTTTTGCAACTAGGTATTTCTAACGCCAGCACAC >OriGene 5' read for NM_001430 unedited
CCCAATCGCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGGCCACACGGGTCCGGTGCCCGCTGCGCTTCCGCCCCAGCGCTCC TGAGGCGGCCGTACAATCCTCGGCAGTGTCCTGAGACTGTATGGTCAGCTCAGCCCGGCC TCCGACTCCTTCCGACTCCCAGCATTCGAGCCACTTTTTTTTTCTTTGAAAACTCAGAAA AGTGACTCCTTTTCCAGGGAAAAAGGAACTTGGGTTCCCTTCTCTCCGTCCTCTTTTCGG GTCTGACAGCCTCCACCCACTCCTTCCCCGGACCCCGCCTCCGCGCGCAGGTTCCTCCCA GTCACCTTTCTCCACCCCCGCCCCCGCACCTAGCCCGCCGCGCGCCACCTTCCACCTGAC TGCGCGGGGCGCTCGGGACCTGCGCGCACCTCGGACCTTCACCACCCGCCCGGGCCGCGG GGAGCGGACGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAGGTGCTCGGCGTCTGAA CGTCTCAAAGGGCCACAGCGACAATGACAGCTGACCAGGAGAAGACAAGGAGTAGCTCGG AGAGGTAGAAGGAGAAGTCCCTGGATGCTGCGCGGTGCCTGCCGGAGCAGGAGACGGAGG TGTTCTATGAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTGAGCTCCCATCTGGACC AGGTCTCCATCATGCGACTGGCAATCAGTTTCCTGGGACACACAGCTCCTCTCCTCAGAT TGCTCTGAAAACGAGTCCGAAGCCCCAGCTGACCACCAGAGGGATAACTTGTACCTGAAG CCTTTGAAGGTT >OriGene 3' read for NM_001430 unedited
CACTGGGGGCAGGGGTACACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGA CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGATCAAGATACTTTAA TAAGATACCAGTGTCAAAATACATTTCCTTATAAAGTTAAGCTCCCATACAGTTATAATG TTGTCTGTTGGAATTCGACAATATAATAACGTTCATGAAATCGTTACGTTGACAGGTAGG GTTAATATGAAGCTTGGAATATTTTTCAGTGTTTTAGTAAAACTGCAAGGGTAAAATGCC CTTAATGCCAGGGCAACACACACAGGAAATCAAATACCAGCATTTACACGTCAGTAACCC TTCAAGTTCTGCCACCCTGTGTGGGGTAATGCCGTGCAGCTAAAATATGATTTACGCAAC ACCATGACTAAGGAATTTCTCATAGAACTTAAATTTCTTTTGAAAGCTATTTTTTGTTTT TGGCAATAAGTCTATCCGGGCTTACTAAATAGTTGGCCCAATGTGCTTTGTGTGTGTTTT TAGAAACTTCTTCATTGTACCCATTACAGAAAAGTTCCATGTTATTGGTATTGAAAAACC AGAGGTGTCGTCCCTCTTACCCAGGGAGTTGCAAAGTGCTCTGGGAACACTATTTTCAGT GCTTCCTACCTACATGTCACTGACCGACCCATAGACCTCAGCCAGGGCCTGTGGCTAGGG GCCATCCCTGGAAGCCTTGGGGGCCATCTGCCTGCCGGCTGAATGGGGGCTGCAGGGCTG GTCTTGGACAGCTGCTCTCCCGTTGTGCAGCCTTCTTCTAAGTCCACCTCCAGGGGCTCC AAGGTCTGAACTTGAAAGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001430 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001430.3, NP_001421.2 |
| RefSeq Size | 5186 bp |
| RefSeq ORF | 2613 bp |
| Locus ID | 2034 |
| UniProt ID | Q99814 |
| Domains | PAS, HLH, PAC |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma |
| Gene Summary | This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Direct eNAMPT Involvement in Pulmonary Hypertension and Vascular Remodeling: Transcriptional Regulation by SOX and HIF2α
,Sun, X;Sun, BL;Babicheva, A;Vanderpool, R;Oita, RC;Casanova, N;Tang, H;Gupta, A;Lynn, H;Gupta, G;Rischard, F;Sammani, S;Kempf, CL;Moreno-Vinasco, L;Ahmed, M;Camp, SM;Wang, J;Desai, AA;Yuan, JX;Garcia, JGN;,
Am. J. Respir. Cell Mol. Biol.
,PubMed ID 32142369
[EPAS1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208604 | EPAS1 (Myc-DDK-tagged)-Human endothelial PAS domain protein 1 (EPAS1) |
CNY 9,560.00 |
|
| RC208604L1 | Lenti ORF clone of Human endothelial PAS domain protein 1 (EPAS1), Myc-DDK-tagged |
CNY 11,960.00 |
|
| RC208604L2 | Lenti ORF clone of Human endothelial PAS domain protein 1 (EPAS1), mGFP tagged |
CNY 5,890.00 |
|
| RC208604L3 | Lenti ORF clone of Human endothelial PAS domain protein 1 (EPAS1), Myc-DDK-tagged |
CNY 11,960.00 |
|
| RC208604L4 | Lenti ORF clone of Human endothelial PAS domain protein 1 (EPAS1), mGFP tagged |
CNY 11,960.00 |
|
| RG208604 | EPAS1 (tGFP-tagged) - Human endothelial PAS domain protein 1 (EPAS1) |
CNY 11,160.00 |
|
| SC320566 | EPAS1 (untagged)-Human endothelial PAS domain protein 1 (EPAS1) |
CNY 9,568.00 |
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