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EDC3 (NM_025083) Human Untagged Clone
CAT＃: SC110646
EDC3 (untagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3
CNY 3,792.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hYjeF_N2-15q23; LSM16; MRT50; YJDC; YJEFN2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110646 sequence for NM_025083 edited (data generated by NextGen Sequencing)
ATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTC TATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCT TTCCATAATGGAGTGAAGTGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACG GAGTTAAAAATTCTGGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCAT CAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAATGGCACA GGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATATCCCTAAGAGG ACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGC TATGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAAC TCCTGGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAG AATGGCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCA GACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAAGGCAGCTGTGTTTGAG GAGATTGATACCTATGAAAGGAGAAGTGGTACCCGTTCCCGGGGCATCCCAAATGAAAGG CCCACTCGGTACCGCCATGATGAGAACATCTTGGAGTCCGAGCCCATTGTCTATCGACGG ATCATAGTGCCCCACAACGTGAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCA AGTATTTCCTATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTGACC CTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACTGACCCTCCTC GGAGGACCTAACAGGTTGAATCCCAAAAATGTTCACCAGAGGCCTACAGTGGCTCTACTG TGTGGACCTCATGTGAAGGGGGCTCAGGGTATCAGCTGTGGAAGGCACCTAGCCAACCAT GATGTCCAGGTCATCCTTTTCCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAAT GAGCTGTCGCTCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGATCTGCCC ACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGAGAACGTCTTCCTGCGC GATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACCAGAACCGGGCACCAGTACTC AGCATAGACCCTCCTGTGCATGAAGTCGAACAGGGCATTGATGCCAAATGGTCACTGGCA CTGGGCCTGCCTCTGCCACTGGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGC ATTCCCCAGCAGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGCTGCAAG TTTGTTATCCCACTGCACTCTGCTTAG Clone variation with respect to NM_025083.3 >OriGene 5' read for NM_025083 unedited
NGTTCNCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGCCGATGCT GTGGGGGTGGGCGTGGAGAGAATTCTTCTGTGGGTCCTCTGGTGTTGAGTGGTCGGCTTG GTGTGGTGTGCGGAGGAGCTCCAGGCCCGTCGGCGCGGAGTGGTCTCACGTGTGAAACAT GGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTA TCAGGGAAGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTT CCATAATGGAGTGAAGTGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGA GTTAAAAATTCTGGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCA AACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAATGGCACAGG CAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATATCCCTAAGAGGAC AGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGCAGCAACAGTGCTCAAAGAGCTA TGTCGACAGGCACATGGAATCCTTGAGTCAGTCCAAAAGTTTCCGTCGTCGGCACAACTC CTGGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAGTGGTTTANAGAAT GGCCAGATGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATGGAGGAGATCCCAGAC CCAGATTTTGATTTTGAAGGGAACCTGGCCTCTTTTTGACAGGCAGCTGTGTTTTGAGGA GATTGATCCCTATGAAAGGAGAAGTGGGTACCCGTTCCCGGGGCATTCCCAATGAAAGGC CCACTCTGTAC >OriGene 3' read for NM_025083 unedited
GGCTTTCAGCTTTCTTTNCAGTGGCTGGTTGTTTGGTTGCCCTAAGAGCTGCATGAGTGC TAGGCTTCACCACGTGACTGGGGGCCCCTTGGGAACTGGGTACTATGGGCAGGATGCCCC TGAAAAGAACTGAAGACAGAGGAATCATACTTCTCTTTAATACCTCTGGGGAAGGCCCAG GCTAAGGATGAGGGCAGGGACCAGTCCCAGTGCCCCCTGGGGAGAGAAGAGGGAGAAGCT TGGGCACAAACTCCCAGTGGCCCTGCAAGGCTATCATCCCTGGATCTTGCTGGAGTGGAC AGTCTTCTGGGCCAGGGTTGTTCTAGCTTGTTTCTCACTGTACCTAATCAGGGGGCCTAA CTGCATTAACAGGCAGCCAGAACCTACCTACCAAAAAGCCTGTAACCTAGACTGCTCCTC AGATTTTGGCCAAGAGAGGGCCCCGGCTTGGGAGCTGCTTGGCCCTGAGAGTGAGCAGAG GCTCAGAGAATACACAGCACAAGGGTTACAGTCCCTGGCCTCTTCCCATCGCTGGAGACA ATTTAAGACTGAAGGGAAGATGAGAGGAGGGGCCAGCCCTCTACCCTGGCTCATCTGCTT CTGGCTATGTTCCTACAAGGGAAGTGATAACAGCCTGAAACCCGTGGTACCAGGGCCTAC CCTATGTCCACAATCCTTGGTGAGCCCTTCTGTCCACCCATTCACCCGCTCAGCCAGCCT GGGCAGACACAGAGCAGAAGCAGCCTGAAGCCCTGAAGCAGGCTTAAACACAAGTCCTTG ACAGGATCCCTGCCAGGATTCAGGGTAGATCCTGTTCTCCAGCCTCCAGGCCTANAGCTG GCACCTTAGATTGAGCTCANTGGGTCCTATGGTGGACCCCACAGCTACTCTCCAGACTAG CCTATTTGCCTCTTGAACAGGGGTGGCCCAGCTCCATTAACTGGCAATGGAAGTTCAACC ACAGAGCANACTGGCGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_025083 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025083.2, NP_079359.2 |
| RefSeq Size | 3755 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 80153 |
| UniProt ID | Q96F86 |
| Domains | YjeF_N |
| Protein Pathways | RNA degradation |
| Gene Summary | This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability. [provided by RefSeq, May 2017] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202321 | EDC3 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3 |
CNY 3,784.00 |
|
| RC202321L1 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3, Myc-DDK-tagged |
CNY 6,184.00 |
|
| RC202321L2 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC202321L3 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202321L4 | Lenti ORF clone of Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG202321 | EDC3 (tGFP-tagged) - Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3 |
CNY 5,384.00 |
|
| SC322393 | EDC3 (untagged)-Human enhancer of mRNA decapping 3 homolog (S. cerevisiae) (EDC3), transcript variant 3 |
CNY 3,792.00 |
