RNF12 (RLIM) (NM_016120) Human Untagged Clone

Name: RNF12 (RLIM) (NM_016120) Human Untagged Clone
SKU: SC110429
Price: 4656.00

CAT＃: SC110429

RLIM (untagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1

Datasheet

SDS

"NM_016120" in other vectors (6)

CNY 4,656.00

货期*

现货

规格

Cited in 2 publications.

Product images

经常一起买 (4)

TA811692

RLIM mouse monoclonal antibody,clone OTI2D7

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MRX61; NY-REN-43; RNF12; TOKAS
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_016120 edited GAATTCGGCACGAGGCCTGAGCAACGTCTCCGAGCAGGCGCTGGGCTAGAGGCGGGTCTC AACCAGCTACTCATTGGAGGCGGGCTTGAGAGCGGCGGCCAGGGAGGTGCGGAGCAGCCT CGGCGGCGGCGGCCGAACCAACCGAGTCGGATCCTGACCCTAAAACCTAGTATTTTCCAC TTGTTCATCAATATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCA GCACAGCGCAGAAGTCAGATGGACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTA AATAACCTGAGTGAAGAAGATTATAGGCTTATGAGAGATAACAATTTGCTAGGCACCCCA GGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAAATTAAAGAAGGCCCACCA CCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTGTCTAATGGTGAC TCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGGCAA AGAGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGA TTCAGTTTAGAGATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAAT GAGCCATCTGCAAGACGTTCTAGTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTG GAAAACCCACGATCTGAATCAACATCTGCAAGGCCATCTAGATCAGAACGAAATTCAACT GAAGCGTTAACAGAGGTCCCACCTACCAGAGGTCAGAGGAGGGCAAGAAGCAGGAGCCCA GACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCACCTCTGCATCCAATGAGT GAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACATCCTTTGGTA AATGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCT GGGCCTGAGTTGCTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAA GGAGCAGGTTCTTCAGACACAGCTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCT CCAACCATAGTCCTTGATCTTCAAGTAAGAAGAGTTCGTCCTGGAGAATATCGGCAGAGA GATAGCATAGCCAGCAGAACTCGGTCTAGGTCTCAGACACCAAACAACACTGTCACCTAT GAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCACGTTCTGAGCGGGCAGGTGTGAGA ACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAATACTGGTTTAAGTGAG ACTACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGTGAGTTA AGCTATTTTATGTACAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAAT ATGGAAAGGGCAGAGTCACGGAGTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGT TCCAGTTCGAGTTCCAGTTCCAGTTCGAGTTCCAGTTCCAGTTCAAGTTCCAGTTCCAGT CCTAGTTCCAGTTCCGGTGGTGAAAGTTCAGAAACTAGCTCAGATTTATTTGAAGGCAGT AATGAAGGAAGCTCATCATCAGGCTCATCAGGTGCCAGGCGAGAGGGTCGACATAGGGCC CCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTTAGCCTGGCTCAGTTTTTCCTC TTAAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAGATTGACAACTTG GCAATGAGAAGTTTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACAGAA TATACAGAAGGCAACAAACTTCGTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGC ATCGATCGCTGGTTATCTGAGAATTCTACCTGTCCTATTTGTCGCAGAGCAGTCTTAGCT TCTGGTAACAGAGAAAGTGTTGTGTAATTAAGATCTGAACTCTCAGCTATGTAGCTGATA TAGTGATGGGCAAACAGGAATCACTTGCTTTTATGTCCACTTTTTGAGTGGTACTTAAAT GTAAAGTAACAACCTGAATTGAGTCATTGCTTTCTGAAGGAATCATTGTCCTTTCTCCAG TTTTTGTTCCAGAATAAAAGGAAATATTTTAAAAGCCACGTTATAGGACCTAAAAATCTG ATTTCCGTACCAGTTAAATTGGTAGCATCACTGTTACTGATAATTTATCATATACACTTG TCAATTTTTCCTTTGTTATCTTAAAAGATCTGCCTTAGCAATGGCTCCTTTTTCATGTTG ATCTTTAAATTTTCCCACACCATAAGAGAGGAGCAAAGGAAATTACCAGTTTAGACTAAG TTACAGTATGTGTTTCATAAGTGATTAAAGCTATATCATTCCCAGTTATTAGTTAACACA AATTCAGCCACATTCTGAGTATTGTTTGTTCACCTTTCAGACTTGGTGATACTGGACATG TCAGTGTAAATAACACTAAGGTTAGGATCTTCTAAGTGTATAACTGTCTCCTAAGCCCAT CACTGTGGCACACTGTAGAGTGAGCTTATAGTTTAATTGAGATATTTATCTTGTGGAAAT ATTAAAAAGCCTATGCTTGTGTAAGTGAAAAAAAATCACATTCATTTGTTTAAAATGTAA AGCTATTTTGTAGAGGCTCAGTACTTTTCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XATGGAGGTGGGGATTGAGAAAAGTGTTCACATTAGTGTTGGAATGTAGGTAATTGTACA GTTTATAAGACCTTTAGCACCAGGAAATAAAATATGTGTGTGTACACACACAGTTTTTAA AAACGATTGGAATTCATCCAAACCTTAAGATTAGGAAACTGATTGGCTGTAAGTGTTGCC TCAAGTAATGTGTCCCCCGCCCCATAACATGTCTTTTCTGTGCCAAGTATCCTCTCCTAG TGTCAGTGTCTCAAATTATATTTATGAAGGGTTATCACATAATATGCTTTGTATTTAACT ACCTGCTTCCCAGGCGTCCATGACATGAAATGGGTGAAGCATCAGCCATGACTTTCGGTC AGTTCCCAAAATACCCAAATGATAGGGAGGTAAAGATTGCAAAATTCCTCTTAAAAGGCC ATTTTTACAGCTTGCTAAAATACAGTGGCACCTTATGGTTGTTAATATTTTTTCTTTTAA AGATGGAGAATGTTTTTATTCTTCCCCTTTTCTCCTTTGTTCTTTTGCGAACAAAAAAAA TTATAATCTGCCTTATTTGATTTTTTTTTTAAGTTTTACCTTACAGGCTTCCCTGTCACA TGTTTTACCATATTAAGTACATAGAAGCCATGTTTGCAGGGCACTTTTATTCTCCTAGTC TTATAAGACTATGCTAATTGTTACTTTCTGAAGTATTCTGTACCTTAGTATGCAAGATTG TTAAGGCACATGTTAAAGTGTCAGGTGTGTTTTTTTCCCTTTAATTCTTCAGTATGTTTA TATTCAATAACAGTATTGTAATTCATTGAGTTAACTAGTAAATTGTGTGCTTTTGAAAAA ACATGAAAAATGTAAATTGACAGCATACTTCATTTGGAAAAAAAAAAAAAAAAAACTCGA C 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_016120 unedited CTATAGGCGGCCGCGNAATTCGCACGAGGCCTGAGCAACGTCTCCGAGCAGGCGCTGGGC TAGAGGCGGGTCTCAACCAGCTACTCATTGGAGGCGGGCTTGAGAGCGGCGGCCAGGGAG GTGCGGAGCAGCCTCGGCGGCGGCGGCCGAACCAACCGAGTCGGATCCTGACCCTAAAAC CTAGTATTTTCCACTTGTTCATCAATATGGAAAACTCAGATTCCAATGACAAAGGAAGTG GTGATCAGTCTGCAGCACAGCGCAGAAGTCAGATGGACCGATTGGATCGAGAAGAAGCTT TCTATCAATTTGTAAATAACCTGAGTGAAGAAGATTATAGGCTTATGAGAGATAACAATT TGCTAGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAAATTA AAGAAGGCCCACCACCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATG TGTCTAATGGTGACTCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAA CAAGAAGTGGGCAAAGAGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACA GTGGTGATTTCAGATTCAGTTTAGAGATAAATGTTAACCGTAATAATGGGAGCCAAAATT CAGAGAATGAAAATGAGCCATCTGCAAGACGTTCTAGTGGAGAAAATGTGGAANACAACA GCCAAAGGCAAGTGGAAAACCCACGATCTGAATCAACATCTGCAAGGCCATCTAGATCAG AACGAAATTCAACTGAAGCGTTAAACAGAGGTCCCACCTACCAGAGGGTCAGAGAGGGGC AGAAGCAAGAGCCCAGACCATCGGAGGACCANGAGCAGAGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_016120 unedited TAGCTTTGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAAATG AAGTATGCTGTCAATTTACATTTTTCATGTTTTTTCAAAAGCACACAATTTACTAGTTAA CTCAATGAATTACAATACTGTTATTGAATATAAACATACTGAAGAATTAAAGGGAAAAAA ACACACCTGACACTTTAACATGTGCCTTAACAATCTTGCATACTAAGGTACAGAATACTT CAGAAAGTAACAATTAGCATAGTCTTATAAGACTAGGAGAATAAAAGTGCCCTGCAAACA TGGCTTCTATGTACTTAATATGGTAAAACATGTGACAGGGAAGCCTGTAAGGTAAAACTT AAAAAAAAAATCAAATAAGGCAGATTATAATTTTTTTTGTTCGCAAAAGAACAAAGGAGA AAAGGGGAAGAATAAAAACATTCTCCATCTTTAAAAGAAAAAATATTAACAACCATAAGG TGCCACTGTATTTTAGCAAGCTGTAAAAATGGCCTTTTAAGAGGAATTTTGCAATCTTTA CCTCCCTATCATTTGGGTATTTTGGGAACTGACCGAAAGTCATGGCTGATGCTTCACCCA TTTCATGTCATGGACGCCTGGGAAGCAGGTAGTTAAATACAAAGCATATTATGTGATAAC CCTTCATAAATATAATTTGAGACACTGACACTAGGAGAGGATACTTGGCACAGAAAAGAA TGTTATGGGGGCGGGGGACACATTACTTGAGGCAACACTTACAGCCAATCAGTTTCCTAA TCTTAAGGTTTGGATGAAATCCAATCGTTTTAAAAACTGTGTGTGTACACACCATATTTT ATTTCCTGGTGCTAAAGGGCTTATAACTGGACAATACCTACATTTCCCACTTATGTGAAA CTTTTCTCAATCCCACTCCATTACCCCATAAGGGGAACTAACATGTCTTTTCAAGCAAAT TCAATAAAAAGGGGCTATTTCTT
Restriction Sites	NotI-NotI
ACCN	NM_016120
Insert Size	6510 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016120.2, NP_057204.2
RefSeq Size	3315 bp
RefSeq ORF	1875 bp
Locus ID	51132
UniProt ID	Q9NVW2
Domains	RING
Protein Families	Druggable Genome, Transcription Factors
Gene Summary	The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (1) lacks an alternate exon in the 5' UTR, compared to variant 2, and may represent the more abundant form. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
Regulation of Estrogen-Dependent Transcription by the LIM Cofactors CLIM and RLIM in Breast Cancer ,Steven A. Johnsen, Cenap Güngör, Tanja Prenzel, Sabine Riethdorf, Lutz Riethdorf, Naoko Taniguchi-Ishigaki, Thomas Rau, Baris Tursun, J. David Furlow, Guido Sauter, Martin Scheffner, Klaus Pantel, Frank Gannon, and Ingolf Bach, Cancer Res., Jan 2009; 69: 128 - 136. [RLIM]
Single-stranded DNA-binding proteins regulate the abundance of LIM domain and LIM domain-binding proteins ,Z Xu, X Meng, Y Cai, H Liang, L Nagarajan, and SJ Brandt, Genes Dev. 2007 Apr 15;21(8):942-55. [RLIM]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC211910	RLIM (Myc-DDK-tagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,648.00
RC211910L1	Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,048.00
RC211910L2	Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC211910L3	Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC211910L4	Lenti ORF clone of Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RG211910	RLIM (tGFP-tagged) - Human ring finger protein, LIM domain interacting (RLIM), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,040.00

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Expression Plasmids

Lentiviral Particles

CRISPR

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Proteins

Antibodies