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PUF60 (NM_078480) Human Untagged Clone
CAT＃: SC110270
PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1
CNY 4,168.00
CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FIR; RoBPI; SIAHBP1; VRJS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_078480, the custom clone sequence may differ by one or more nucleotides
ATGGCGACGGCGACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGG CGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGG GCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTCAGAAGGCC AAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGC AGCTCACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGAT GGCGGCTCAGCGGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTG GGGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACT CCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGCTGGCCTT GGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAAGGTGGGCAGACCCAGCAACATAGGGCAG GCCCAGCCCATCATAGACCAGTTGGCTGAGGAGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGC ACCAGGACCTCTCAGACGATGACATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACT GGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCC CAAGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAAGGCTGTCA CACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGCCGCTGCTGTGGCAGCTGC TGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGGAGCAGCGGTGCTGGGTACCCTGGGCACA CCTGGACTGGTGTCCCCAGCACTGACCCTGGCCCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTG CCCAGGCACCTGGAGTCATCACAGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGT GGGAGTGGTGAACCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAA GAAGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAGCATCTCGG GCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTCTACAGTGATGGTTCTGCG CAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGGGGAGGTGACAGAGGAGTGTGGCAAGTTC GGGGCCGTGAACCGCGTCATCATCTACCAAGAGAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCA AGATCTTTGTGGAGTTTTCCATAGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTT TGCTGGCCGCAAGGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGA >OriGene 5' read for NM_078480 unedited
TATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGACGGACGCA AGATGGCGACGGCGACCATAGCTCTCGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGG CGGCGGCGGCGGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACT CCATCAAGATGGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGC CCGAGCAGCAGGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGA GTGTGCTGGTGAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGG CAGCAGTGACAATGGGCTTTGGAGATCCTCTCTCACCTTTGCAATCGATGGCGGCTCAGC GGCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGG GGGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGT CCTGGGACTCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCG AAGCTGCACAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCA AGGTGGGCAGACCCAGCAACATANGGCANGCCCAGCCCATCATAGACCAGTTGGCTGAGG AGGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGCCCAGGACCTCTCAGACGATGA CATCAAGAGCGTGTTTGTAGCCTTTGGCAAGACAAGTTCTGACACTGGCCCGGGACCCAC ACTGGCAGCACAGGGCTACGCTTCATTGATACGAGAAGCCAGTCGTCCCAGAGCTTGGTC TCCATGACCTCTTGACTGGTGCCAGTACTGCGGTGGGCAGGTGTACACCCCCATGCCT >OriGene 3' read for NM_078480 unedited
TTGGACGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTGCCAGCAGCATCCG CACCTTTATCCGCACTGTAGGCTGGGCTGGGCAGAGCGCGCCTGGCCCCGGGGACACCAC TGTATCACTATAAAACCCAGAGGAAACAAGGAACAAGTGCAAGTCCGGGGAGAGGGACCA CTGTCACGCAGAGAGGTCACTGTTATCAAAACGCTCCTGGTCGTACACTTCAGCCACCAC CTTGCGGCCAGCAAACCAGCGGCCATTGAGGGCCTGGATGGCCTTATGAGTCTCAGAGGC TATGGAAAACTCCACAAAGATCTTGACAATGATTTCTGCATCCTCCTCCTCGCCTTGTTT CTCTTGGTAGATGATGACGCGGTTCACGGCCCCGAACTTGCCACACTCCTCTGTCACCTC CCCTTCCAGGTCATCATCGATGTCCTTGGGGTCCACCATGTTGCGCAGAACCATCACTGT AGACTCCTGCTTGCGGAGCAGCTTCTGCATCACCATGTGTCGGGCGCTACTGCCCGAGAT GCTCATGTGCTCCTGCTCGCTCAGCATCTCTGGCCGCTCTGACTCGGGAAACAGCTCCTC TTCTTCCTTCTCCTTCTTGGGCTCCAGGAGACCCAGCGTTGGAGGGCTGGCCAGGATGGG GTTCACCACTCCCACCGAGGGGATGGTGACCGGGATAGGAGGACGGGCTGGGGTCACACC TGTGATGACTCCANGTGCCTGGGCAGCCATGACAGCCTGGGGCANAGTGCCCCAGGGCTG GGCCAGGGTCAGTGCTGGGGACACCAGTCCAAGTGGCCCAGGGTACCCAGCACCGCTGCT TCGGCCACTGCTTTCTGAGCTGTGATCTTGGCAATGGCTGCANCANCTGCCACAACAACG GCAGGTGGGAAGGCCCTCCAGCGTGCTGGTGTGAATAAGGGCATGGNGCGGGTGAANCCT TTGCCAACCGCAGTACTGGGCCACCAGTTCAAAAAGTTCATGGAAAAACCACATCTTGGG ACAACGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_078480 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_078480.1, NP_510965.1 |
| RefSeq Size | 1946 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 22827 |
| UniProt ID | Q9UHX1 |
| Domains | RRM, RRM_1 |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (1) encodes the isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223492 | PUF60 (Myc-DDK-tagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 |
CNY 4,152.00 |
|
| RC223492L3 | Lenti ORF clone of Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC223492L4 | Lenti ORF clone of Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG223492 | PUF60 (tGFP-tagged) - Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 1 |
CNY 5,040.00 |
