MID2 (NM_052817) Human Untagged Clone

CAT#: SC110211

MID2 (untagged)-Human midline 2 (MID2), transcript variant 2



  "NM_052817" in other vectors (6)

CNY 13,590.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


MID2 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms FXY2; MRX101; RNF60; TRIM1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_052817, the custom clone sequence may differ by one or more nucleotides


ATGGGTGAAAGCCCAGCCTCCGTGGTTCTTAATGCCTCAGGAGGACTATTTTCACTAAAGATGGAAACAC
TGGAGTCTGAATTGACCTGTCCAATCTGCCTAGAGTTGTTTGAAGACCCCCTTCTGCTCCCTTGTGCTCA
CAGCCTCTGCTTCAGCTGTGCCCATCGCATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCC
ATTACTGCTTTCCAGTGTCCTACCTGCAGGTATGTTATCTCGCTGAACCACCGGGGCCTGGATGGCCTCA
AGAGGAATGTGACTCTGCAGAACATTATTGATCGCTTCCAGAAGGCTTCAGTCAGTGGGCCCAATTCCCC
TAGTGAGAGCCGCCGGGAAAGGACTTACAGGCCCACCACTGCCATGTCTAGCGAGCGAATTGCTTGCCAA
TTCTGTGAGCAGGACCCGCCAAGGGATGCAGTAAAAACATGCATCACCTGTGAGGTCTCCTACTGTGACC
GTTGCCTGCGGGCCACGCACCCCAACAAGAAACCTTTCACCAGCCACCGCCTGGTGGAACCAGTGCCAGA
CACACATCTTCGAGGGATCACCTGCCTGGACCATGAGAATGAGAAAGTGAACATGTACTGTGTATCTGAT
GACCAATTGATCTGTGCCTTATGCAAACTGGTGGGTCGTCACCGAGACCATCAGGTCGCATCCCTGAATG
ATCGATTTGAGAAACTCAAGCAAACTCTGGAGATGAACCTCACCAACCTGGTTAAGCGCAACAGCGAACT
AGAAAATCAAATGGCCAAACTAATACAGATCTGCCAGCAGGTTGAGGTGAATACTGCTATGCATGAGGCA
AAACTTATGGAAGAATGTGACGAGTTGGTAGAGATCATCCAGCAGAGGAAGCAAATGATCGCTGTCAAAA
TCAAAGAGACAAAGGTTATGAAACTGAGAAAGTTGGCACAGCAGGTTGCTAATTGCCGCCAGTGTCTTGA
ACGGTCAACAGTCCTCATCAACCAAGCTGAGCATATCCTGAAAGAAAATGACCAGGCACGGTTTCTACAG
TCTGCAAAAAATATTGCTGAGAGGGTCGCTATGGCAACTGCATCTTCTCAAGTTCTGATTCCAGACATCA
ATTTTAATGATGCCTTTGAAAACTTTGCTTTAGATTTTTCCAGAGAAAAGAAACTGCTAGAGGGGTTAGA
TTATTTAACAGCCCCAAACCCACCATCTATCCGAGAAGAACTCTGTACTGCCTCCCATGACACCATTACA
GTCCACTGGATCTCGGATGATGAGTTCAGCATCAGCTCCTATGAGCTTCAGTACACCATATTCACTGGCC
AGGCTAACTTCATCAGCCTGTATAATTCAGTAGACAGCTGGATGATTGTTCCCAACATTAAACAGAACCA
TTACACAGTGCATGGACTCCAGAGCGGGACTCGCTACATCTTCATCGTTAAAGCCATAAACCAAGCCGGC
AGCCGGAACAGTGAACCTACCCGACTAAAAACAAACAGCCAACCCTTTAAATTGGATCCCAAAATGACTC
ACAAGAAGTTGAAGATCTCCAATGATGGATTGCAGATGGAGAAGGATGAAAGCTCTCTAAAGAAGAGCCA
CACCCCAGAGAGGTTTAGTGGCACAGGGTGCTATGGGGCAGCAGGAAATATATTCATTGACAGTGGCTGC
CACTATTGGGAGGTGGTCATGGGTTCCTCAACATGGTATGCAATTGGCATTGCCTACAAATCAGCTCCAA
AGAATGAATGGATTGGCAAGAATGCCTCCTCATGGGTCTTCTCTCGCTGCAATAGTAACTTCGTGGTGAG
ACACAACAACAAGGAAATGCTGGTGGATGTGCCCCCACACCTGAAGCGTCTGGGTGTCCTCCTGGATTAT
GACAACAATATGCTGTCTTTCTATGACCCAGCTAACTCTCTCCATCTTCATACTTTTGATGTGACCTTCA
TTCTTCCAGTTTGTCCAACATTTACAATCTGGAACAAATCCCTAATGATCCTGTCTGGCTTGCCTGCCCC
AGATTTTATTGATTACCCTGAGCGGCAGGAATGCAACTGCAGGCCTCAAGAATCCCCTTATGTTTCTGGG
ATGAAAACCTGTCATTAA


>OriGene 5' read for NM_052817 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTCTCCGGTCACT
CCTGGCCAGGGGAGAAGACATGTAAACGTGCCTCCAGAGGAAGGGCTGGGGGAGCTGGGA
GTGGCGGGGGCGGCGGAAGGTGAGCCTGGGAAGGTGGCCGAGCACCCCTTCTTCTTGGGC
CCTTCCCAGTGCTCCCTTGGGGTGTCAGCCCCAGCCCCGTTTGCCCCCACTCCGCCTCCC
TTTTGGAAGGGATTGCCTTTTTTTTCCTCTGCGGCGGCGGAAATGACAGTGTGGTGCTGC
CGTGGTGTCATGGTGCTGCCCCTGGACTGAGGGGCGAAAACTCTTAAGTTTAGCTCGGGA
GGCCCAGCTGCGGTAGCATCGCGGCCGCCGTGCTGTCCCCTGCGGGGCGCGCGGGCTGGC
GGATCCCGGCTGCTGCGCGGCGTCGGCGACGGTAGCGGCAGCGGCGGCGAAGGCGGGCGG
CGGCCTACAGTGGTAGCGGCGGCGGCGGCGACCGGGGCCCGGGAGCTCGCGCCGGAGCCC
GAGCCAACCCGCTGCGGAGGCAGACGAGAGCCCAGCGCCCTCGAGCGAGCGGAGGAGATG
GCTGGCACCTGGGAACGCTATGGGTGAAAGCCCACCTCCGTGGTTCTTAATGCCTCAGGA
GGACTATTTTCACTAAAGATGGAAACACTGGAGTCTGAATTGACCTGTCCAATCTGGCCT
AGAGTTGTTTGAAGACCCCTTCTGCTCCCTTGTGCTCACAGCCTCTGCTTCAGCTGTGCC
ATCGNNATTTTGGTATCAAGCTGCAGCTCTGGTGAATCCATTGAACCCATTACTGCTTTC
CAGTGTCTACTGGGGAGAGTATGTATCTCGCTGACCACCGGNNCCCTGNATGGCCTCAAA
GAGAATGTGACTCTGCAGACNATTATTGATCGCTTCCAGAGGCTCN
>OriGene 3' read for NM_052817 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGATGCTTCAAACAAACT
TTATTTTGTGTCTCGTATATTGAACGCTAACTTAGGTGTAGGAGGGGAAGAACTGCTGAA
CTAGAGAGCCAGTGAATTATATACTCTCCTGAAACTTAATGACAGGTTTTCATCCCAGAA
ACATAAGGGGATTCTTGAGGCCTGCAGTTGCATTCCTGCCGCTCAGGGTAATCAATAAAA
TCTGGGGCAGGCAAGCCAGACAGGATCATTAGGGATTTGTTCCAGATTGTAAATGTTGGA
CAAACTGGAAGAATGAAGGTCACATCAAAAGTATGAAGATGGAGAGAGTTAGCTGGGTCA
TAGAAAGACAGCATATTGTTGTCATAATCCAGGAGGACACCCAGACGCTTCAGGTGTGGG
GGCACATCCACCAGCATTTCCTTGTTGTTGTGTCTCACCACGAAGTTACTATTGCAGCGA
GAGAAGACCCATGAGGAGGCATTCTTGCCAATCCATTCATTCTTTGGAGCTGATTTGTAG
GCAATGCCAATTGCATACCATGTTGAGGAACCCATGACCACCTCCCAATAGTGGCAGCCA
CTGTCAATGAATATATTTCCTGCTGCCCCATAGCACCCTGTGCCACTAAACCTCTCTGGG
GTGTGGCTCTTCTTTAGAGAGCTTTCATCCTTCTCCATCTGCAATCCATCATTGGAGATC
TTCAACTTCTTGTGAGTCATTCTGGGATCCAATTTAAAGGGTCTGCTGTTTGTTTTTAGT
CCGGTAGGCTCACTGTTCCCGCTGCCCGCTTGGTTNATGGCCTTTACGATGAAGATGTAC
CGAGTCCCCCTCTGGAGCCATGCCCTGNGAATGGNTCTGNNTAATGTTGGAACATCATCC
CACTGTCTACTGGATATACAGCTGATGAAGTACCCTGCCA
Restriction Sites NotI-NotI     
ACCN NM_052817
Insert Size 4150 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_052817.1, NP_438112.1
RefSeq Size 7255 bp
RefSeq ORF 2058 bp
Locus ID 11043
UniProt ID Q9UJV3
Domains zf-B_box, RING, BBC, SPRY, FN3
Protein Families Druggable Genome
Gene Summary The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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